摘要/Abstract
综合运用硅胶柱色谱、反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱技术对药用植物巴戟天内生真菌Trichoderma spirale A725的次生代谢产物进行分离纯化，得到6个聚酮类化合物，采用多种现代波谱技术确定其结构，分别为：6-羟基-4-异丙基-1，8-二甲基螺环[4.5]癸-1，8-二烯-7-酮（1），2-羟基-2，5-二甲基-7-氧代-5，7-二氢-2H-呋喃[3，4-b]吡喃-4-羧酸（2），3-乙基-4-羟基-6-甲基-二氢-吡喃-2-酮（3），苯乙内酯A（4），3-羟基-5-（4-羟基苄基）二氢呋喃-2（3H）-酮（5），4-乙酰-3-羟基-6-甲基吡喃-2-酮（6）.其中化合物1和2为新化合物，化合物3为新天然产物.此外，利用四株肿瘤细胞株（HepG-2、MCF-7、SF-268及A549）对化合物1~6细胞毒活性进行评估，结果表明化合物1~6对上述肿瘤细胞均无明显的细胞毒活性.
关键词: 巴戟天, 内生真菌, 螺旋木霉, 聚酮
Six polyketides were isolated from the fermentation broth of an endophytic fungus Trichoderma spirale A725 of the medicinal plant Morinda officinalis by silica gel, reversed phase silica column chromatography, sephadex LH-20 and high performance preparative liquid chromatography. Their structures were elucidated to be 6-hydroxy-4-isopropyl-1,8-dimethyl-spiro[4.5]deca-1,8-dien-7-one (1), 2-hydroxy-2,5-dimethyl-7-oxo-5,7-dihydro-2H-furo[3,4-b]pyran-4-carboxylicacid (2), 3-ethyl-4-hydroxy-6-methyl-2H-pyran-2-one (3), harzialactone A (4), 3-hydroxy-5-(4-hydroxybenzyl)dihydrofuran-2(3H)-one (5), and 4-acetyl-3-hydroxy-6-methyl-pyran-2-one (6) on the basis of extensive spectral analysis. Among them, compounds 1 and 2 were new compounds, and compound 3 was a new natural product. Moreover, the cytotoxicities of compounds 1~6 were evaluated against four cancer cell lines (Hep G-2, MCF-7, SF-268 and A549). However, none of them exhibited cytotoxic activity against the tested tumor cells.
Key words: Morinda officinalis, endophyte fungus, Trichoderma spirale, polyketide
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