摘要/Abstract

一氧化碳（CO）是一种重要的内源性气体递质分子，参与调节生命体的多种生理和病理过程.因此，选择性识别和高灵敏检测生物体内源CO具有十分重要的生物学和医学意义.荧光探针法具有选择性好、灵敏度高、适于高通量筛选，尤其是对生物样品无侵入性损伤，以及可实现实时原位检测等优势，因此，利用荧光探针技术检测细胞、组织和活体内CO浓度的变化是近年来研究热点之一.综述了近十年来CO荧光探针的研究进展，概述了相关荧光探针的设计理念、检测机理及生物应用，探讨了探针的结构和性能之间的关系，展望了CO荧光探针的发展趋势和应用前景.
关键词: 一氧化碳, 气体递质, 荧光探针, 荧光成像
As an important endogenous gas transmitter, carbon monoxide (CO) has been found taking part in multiple physiological and pathological processes for life. Therefore, the selective recognition and sensitive detection of CO are of great biological and medical significance. Fluorescent probe method is highlighted by its good selectivity, high sensitivity, suitable for high-throughput screening, especially noninvasive detection, and real-time monitoring in situ. Therefore, the development of fluorescent probes for intracellular CO has been becoming one of the hot topics. Herein, the progress during the last decade of fluorescent molecular probes based on the small molecules for CO detection is reviewed. These fluorescent probes are classified and concluded according to the design concepts, detection mechanism, and biological applications. In addition, the relationship between molecular structures and properties is elucidated. Finally, the challenge and application prospects for the development of CO fluorescent probes are also discussed.
Key words: carbon monoxide, gasotransmitter, fluorescent probe, fluorescent imaging


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