摘要/Abstract

利用硅胶柱层析和半制备高效液相色谱（HPLC）等色谱分离技术对拟热带灵芝的化学成分进行分离纯化，从中分离得到了3个新的酚酸杂萜类化合物（ganoduriporols C~E）.运用NMR和HRESIMS等多种波谱技术鉴定了它们的结构.对所分离的化合物进行体外PTP1B抑制活性测试，结果显示所得化合物具有明显的蛋白酪氨酸磷酸酶1B（PTP1B）抑制活性，其IC₅₀值分别为19.1，17.8和29.6 μmol·L^-1.
关键词: 拟热带灵芝, 杂萜成分, PTP1B抑制活性
Three new meroterpenoids of ganoduriporols C~E were isolated from the fruiting bodies of Ganoderma ahmadii Steyaret by silica gel column, high performance liquid chromatography (HPLC) and other chromatography methods. Their structures were determined by spectroscopic techniques including NMR and HR-ESI-MS. Their inhibitory activities for protein tyrosine phosphatase 1B (PTP1B) in vitro were tested. The results showed that the three compounds had obvious PTP1B inhibitory activitiy with the IC₅₀ values of 19.1, 17.81, and 29.6 μmol·L^-1, respectively.
Key words: Ganoderma ahmadii Steyaret, meroterpenoid compound, PTP1B inhibitory effect


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