摘要/Abstract

以6-氟-7-氨基香豆素为荧光基团，氟代叠氮和7-硝基苯并氟咱（NBD）-哌嗪为H₂S反应基团和荧光淬灭基团，合成一种双反应H₂S荧光探针.探针对H₂S的识别性质研究表明，探针的两个反应基团与H₂S响应速率匹配，探针对H₂S具有高选择性和灵敏性，其荧光增强约3600倍，检测极限为4.0×10^-8 mol/L.酶活性测试表明，探针可用于胱硫醚β合成酶（CBS）酶活性检测和抑制剂筛选.细胞成像实验表明，探针可用于细胞内H₂S的成像研究.
关键词: 硫化氢, 响应速率, 双反应, 荧光探针
A dual-reactable H₂S fluorescent probe was designed and synthesized by employing ortho-fluoro-substituted coumarin azide and 7-nitrobenzofurazan-piperazine as the H₂S reactive groups and the fluorescence quenching groups. The recognition behaviors of the probe to H₂S were investigated and the results showed that the probe exhibited high selectivity and sensitivity. The fluorescence off-on enhancement was ca. 3600-fold, and the detection limit was 4.0×10^-8 mol/L. The results of enzyme activity test indicated that the probe could be used for cystathionine β-synthase (CBS) activity detection and inhibitor screening. Furthermore, the probe was successfully applied for the imaging of H₂S in living cells.
Key words: hydrogen sulfide, response rate, dual-reactable, fluorescent probe


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