摘要/Abstract

设计并合成了一种基于8-羟基喹啉（8-HQ）的连续检测Cd²⁺和焦磷酸阴离子（PPi）的荧光传感器L.在传感器L的DMSO/H₂O（V/V=1/1，0.01 mol/L，Hepes-HCl buffer，pH=7.20）溶液中加入Cd²⁺后导致荧光发射峰（Em=537 nm）猝灭，检测限低至5.87×10^-8 mol/L.通过Job's曲线图和质谱验证传感器L和Cd²⁺离子之间以1：1化学计量比结合，结合常数为4.38×10⁴ L/mol.复合传感器L-Cd²⁺体系具有通过配体置换法对PPi高度选择性检测性能.L可以作为一种逻辑门荧光传感器检测Cd²⁺和PPi.传感器L可以用于对活细胞中的Cd²⁺和PPi的荧光成像.
关键词: 8-羟基喹啉, 荧光传感器, 连续检测, 活细胞成像
A novel fluorescent sensor L based on 8-hydroxyquinoline (8-HQ) has been designed and synthesized. The sensor L displayed excellent sensitivity specific toward Cd²⁺ and pyrophosphate anion (PPi), respectively. The fluorescence emission peak at 537 nm was quenched when Cd²⁺ was added to the DMSO/H₂O (V/V=1/1, 0.01 mol/L, Hepes-HCl buffer, pH=7.20) solution of sensor L and the detection limit was as low as 5.87×10^-8 mol/L. The 1:1 stoichiometry was verified by Job's plot and mass spectrum, and the association constant was obtained to be 4.37×10⁴ L/mol. The further study found that the L-Cd²⁺ system has high selectivity for detection of PPi by ligand replacement method and L could be used as a logic gate fluorescence sensor. In addition, the sensor L was employed for imaging the Cd²⁺ and PPi in living cells.
Key words: 8-hydroxyquinoline, fluorescent sensor, sequential detection, cell imaging


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