摘要/Abstract

水合肼（N₂H₄）是一种高毒性水溶生化试剂，具有致癌、致畸、致突变的能力.为了准确检测环境及生命体内N₂H₄浓度，基于荧光共振能量转移（FRET）原理，设计合成了两个双发射比例计量型探针（FRET-1/2），探针结构均经核磁共振氢谱、碳谱和高分辨质谱确证.研究结果表明，两个探针对N₂H₄均表现出较高的选择性和良好的灵敏性，可用于检测水样中N₂H₄浓度.
关键词: 水合肼, 荧光共振能量转移(FRET), 比例计量, 荧光探针
Hydrazine (N₂H₄) is a highly toxic biochemical reagent with the capability of mutagenic, teratogenic and carcinogenic. For accurately monitoring the concentration of N₂H₄ in the environment and life, two FRET (fluorescence resonance energy transfer)-based dual-emissive ratiometric fluorescent probes (FRET-1/2) were designed and synthesized. The structures of both probes were charactered by ¹H NMR, ¹³C NMR and HRMS. The results prove that both probes exhibit good selectivity and sensitivity to N₂H₄ and can be used for detecting N₂H₄ in water samples.
Key words: hydrazine, fluorescence resonance energy transfer (FRET), ratiometric, fluorescent probe


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