摘要/Abstract

设计合成了一种基于异长叶烷酮的新型pH荧光探针7-（4'-二甲氨基苯亚甲基）异长叶烷酮（DB），并用氢谱、碳谱、质谱等方法对其结构进行表征.采用紫外吸收光谱（UV）和荧光发射光谱（FL）光谱研究了该探针对H⁺的识别性能.结果表明，DB是对H⁺具有高度选择性和可逆性的荧光探针，其荧光强度可以在一个相对较低的酸性pH范围内线性地降低.此外，DB对H⁺的专一识别能力不会受到金属离子的干扰.DB的荧光强度在酸性pH值1.0～3.5的区间内呈现出良好的线性关系，其线性回归方程为：I=174.134pH－47.836，R²=0.9936.DB在固体状态时依旧可以快速地探知环境中的三氟乙酸（TFA）气体.而且，该pH探针还成功地用于HeLa活细胞内的荧光显微成像.
关键词: 异长叶烷酮, 极端pH荧光探针, 荧光成像
A novel pH probe 7-(4'-(dimethylamino)benzylidene)-isolongifolanone (DB) was designed and synthesized from isolongifolanone and characterized by ¹H NMR, ¹³C NMR, and MS. The recognition properties of the probe towards H⁺ were investigated by UV-Vis and fluorescence spectroscopy. The results showed that DB exhibited highly selective and sensitive fluorescence response towards H⁺ ion, and the fluorescence intensity decreased linearly in a relatively low range of acidic pH. In addition, the recognition ability of DB towards H⁺ was not affected by various metal ions. The fluorescence intensity of DB had a linear relationship in the acidic pH range of 1.0～3.5, I=174.134pH－47.836, R=0.9936. DB was also sensitive to change in surrounding trifluoroacetic acid (TFA) vapor. Moreover, the pH probe was successfully applied to imaging extreme acidity in HeLa cells.
Key words: isolongifolanone, fluorescent probe for hash pH, fluorescent imaging References


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