摘要/Abstract

设计合成了一个新的罗丹明席夫碱类荧光探针1，其结构经¹H NMR、¹³C NMR、MS和X射线单晶衍射的验证.通过紫外-可见光谱法和荧光光谱法研究了探针1在乙醇-水（V/V=7/3，HEPES 10 mmol/L，pH=7.0）中对Hg²⁺的识别性能.探针1通过显著的荧光增强来识别Hg²⁺，并具有良好的选择性和抗干扰能力.通过Job's plot和MS证明了探针1和Hg²⁺形成1：1的配合物.探针1的荧光强度与Hg²⁺浓度（0~50 μmol/L）呈良好的线性关系，可以定量地检测该范围内的Hg²⁺.在MGC-803活细胞中的荧光显微成像表明，探针1可检测生物体内的Hg²⁺.
关键词: 罗丹明B, 合成, 荧光探针, Hg²⁺, 生物成像
A novel rhodamine B Schiff-base fluorescence probe 1 was synthesized and characterized by ¹H NMR, ¹³C NMR, MS and single-crystal X-ray analysis. The recognition properties of the probe 1 with Hg²⁺ ions were investigated in ethanol-water (V/V=7/3, HEPES 10 mmol/L, pH=7.0) by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The probe 1 showed remarkable "turn-on" fluorescent responses to Hg²⁺ with good selectivity over the other metal ions examined. The 1:1 binding model of probe 1 with Hg²⁺ was established by Job's plot and MS spectra. The probe can be applied to the quantitative analysis of Hg²⁺ with a linear range covering from 0 to 50 μmol/L. The fluorescence imaging experiments in MGC-803 living cells demonstrated that 1 could be used in biological systems to monitor Hg²⁺.
Key words: rhodamine B, synthesis, fluorescent probe, Hg²⁺, bioimaging


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