摘要/Abstract

以罗丹明为基础合成、表征了一种"turn-on"型荧光探针.该探针对Fe³⁺表现出很高的选择性和灵敏性.将Fe³⁺加入到探针中，溶液的紫外吸收值和荧光强度都有很明显的变化，同时伴随着肉眼可见的溶液颜色改变.研究发现，探针与Fe³⁺按照1：1进行络合，引起了探针内酰胺环的打开，进而吸收值和荧光强度值发生变化.研究表明，在有其他共存离子存在下，该探针仍然可以有效检测Fe³⁺.细胞实验发现，探针可以穿过细胞膜，在细胞内与Fe³⁺作用，同时也可检测小鼠体内Fe³⁺，呈现出很好的荧光成像效果.
关键词: 荧光探针, Fe³⁺, 光谱性质, 机理, 细胞和小鼠
A turn-on probe, which exhibited highly selective and sensitive signaling behaviors toward Fe³⁺ over other common metal ions, has been synthesized and confirmed by ¹H NMR, ¹³C NMR, IR and MS. The addition of Fe³⁺ to the solution of probe induced a remarkable UV-Vis and fluorescence enhancement along with obvious color change detected by the naked eye. The reaction mechanism was also investigated and proposed as that the probe complexed Fe³⁺ via a 1:1 binding mode, and the subsequent complexation caused the ring-opening of the rhodamine spirolactam and thereby the changes of absorbance and fluorescence. These remarkable properties may allow Fe³⁺ to be detected directly in the presence of the other examined competing metal ions. Then, cells experiments showed that the probe could permeate through cell membranes and react to Fe³⁺ within living cells. Additionally, the probe was further used for detecting Fe³⁺ ions in living mouse which exhibited excellent fluorescence imaging.
Key words: fluorescent probe, Fe³⁺, spectral properties, mechanism, cells and mouse


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