摘要/Abstract
氟硼荧（BODIPY）类亲水性生物荧光探针近年来在结构优化和功能修饰方面取得了长足进步，因其水溶性修饰方法的不断优化，在活体生物荧光成像领域的应用研究日趋活跃，逐步成为新一代高性能生物成像材料.以水溶性修饰方法为主线，按照离子型、中性、两亲性BODIPY亲水性生物荧光探针分类，系统综述了近年来，重点是2006年以来BODIPY类亲水性荧光染料在生物体内各类必需及致病化学成分检测、癌细胞早期发现和干预、药物递送示踪、特定细胞器标记、胞内各类生物化学反应和性质变化实时监测、光动力治疗等多项生物学和医学领域的应用及研究进展，归纳了各类水溶性修饰方法的优劣及现阶段研究中存在的主要问题，并提出了未来发展方向.
关键词: 氟硼荧(BODIPY), 生物荧光探针, 水溶性, 生物成像
Recently, the fluorescent bio-probe based on water-soluble boron-dipyrromethene (BODIPY) obtains enormous progress on structural optimization and functional modification, and becomes novel high-performance biological imaging materials owing to the development of hydrophilic modification. Focusing on the methods for improving the hydrophilicity of BODIPY, the water-soluble BODIPYs have been classified as ionic probe, electrically-neutral probe and amphiphilic probe. The research progress on the application of water-soluble BODIPY dyes in field of biology and medicine since 2006 has been summarized in this review, such as detection of necessary and pathogenicbiological chemical components, early detection and intervention of cancer, labelling drug delivery, marking specific organelles, real-time monitoring of biochemical and property changes with cells, andphotodynamic therapy. The merits of methods for improving the hydrophilicity and main issues of current studies have been summed up. The hypothesis for future directions has also been put forward.
Key words: boron-dipyrromethene (BODIPY), fluorescent bio-probe, water-soluble, biological imaging
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