Alexandre Grassart 研究员 | 博士生导师

学科

课题组 生物工程与微生物研究组




电话 +86
邮箱
地址 上海市岳阳路320号生命科学实验楼B802

研究方向
Unit of Bioengineering and Microbiology





个人简介 2020-Present: Professor and Principal investigator
Institut Pasteur Shanghai - Chinese Academy of Sciences
Center for Microbes, Development and Health
Shanghai, China
2014-2019: Senior Post-doctoral Fellow
Institut Pasteur Paris - INSERM
Unit of Molecular Microbial Pathogenesis
Paris, France
2010-2014 Post-doctoral Fellow
University of California Berkeley
David Drubin Lab
Berkeley, USA
2006-2010 Ph.D
Institut Pasteur Paris - University Paris-Saclay (Paris 11)
Unit of Biology of Cell interactions
Paris, France
2006 M.S
University Paris Descartes (Paris 5)
Paris, France


学术兼职

发表论文和专利 1 Valérie Malardé, Grassart Aand Sauvonnet N^#. Submitted (Nature communication)Bioengineered human organ-on-Chip reveals intestinal microenvironment and mechanical forces impacting Shigellainfection.2.Grassart A^#, Valérie Malardé, Gobaa S, Anna Sartori-Rupp, Jordan Kerns, Katia Karalis, Benoit Marteyn, Sansonetti P and Sauvonnet N^#. Cell Host & Microbe(2019) Sep 11;26(3):435-444. Bioengineered human organ-on-Chip reveals intestinal microenvironment and mechanical forces impacting Shigellainfection.
# Corresponding authors
2. Ferrari M, Malardé V, Grassart A, Salavessa L, Nigro G, Decorps-Leclere S, Masson V, Arras G, Loew D, Rhode J, Sansonetti Pj, Sauvonnet N. Shigellaflexneri induces a global blockage in host cell intracellular transport leading to cell and tissue disorganization. PNAS.(2019) Jul 2; 116(27):13582-13591.
3. Bertot L*, Grassart A*, Lagache T*, Nardi G*, Basquin C, Olivo-Marin JC and Sauvonnet N. Cell reports(2018) Feb 6;(22):1574-1588. Quantitative imaging and statistical analysis of the dynamics of clathrin-dependent and -independent endocytosis reveal a differential role of endophilinA2 in dynamin2 recruitment.
*These authors contributed equally to this work

4. Lagache T, Grassart A, Faklaris O, Sauvonnet N, Danglot L, Olivo-Marin JC. SODA: A statistical framework to analyze the spatial organization of molecules in multi-color super-resolution microscopy. Nature Communications(2018) Feb 15;9(1):698.

5. Grassart A*, Cheng AT*, Sun Hae Hong, Fan Zhang, Nathan Zenzer, Yongmei Feng, David M. Briner, Gregory D. Davis, Dmitry Malkov, Drubin DG. Actin and dynamin2 dynamics and interplay during clathrin mediated endocytosis. J Cell Biol.(2014) Jun 205 (5):721-735.
*These authors contributed equally to this work


科研进展和成果 We established our lab at IPS in Oct 2020. By pioneering the use of intestine on chip technology for studying host-pathogen interactions, we recently demonstrated a key role of mechanical forces in enteroinvasive pathogen infection, in particular Shigella. We are now investigating the molecular machinery involved in the mechano-sensing and mechano-transduction mechanisms allowing pathogen cell invasion and propagation.

奖励荣誉 Shanghai thousand talents program

实验室介绍 Our laboratory is exploring the interactions happening at epithelial interfaces between microbes, host cells and microenvironment. In particular, we seek to elucidate how this network is dynamically integrated at the gut interface to function as a defensive barrier against pathogen infections.
A first aspect of our research aims at understanding the spatio-temporal organization of gut microbial communities to gain new insights into the protective role of gut microbiota against pathogens, such as Shigella flexneri. A second aspect of our research aim at understanding how tissue microarchitecture and mechanical forces play a role in the maintenance of the intestinal microbial ecosystem and how these physical cues impact host-pathogen interactions.
To pursue these goals, we are applying cutting edge strategies in stem cell bioengineering, genome-engineering, microfluidics and microfabrication to build ex vivohuman mimetic organs, called organ on chip. The high physiological relevance of these microfluidic models allow us to better model infectious processes and open new avenues for personalized medicine and drug discoveries.

实验室成员(Lab member)
Lab manager: Chen xiuman
Research assistant: Wang Rui

招生信息(admission information)
欢迎具有生物学、微生物学等学科背景的优秀学生加入本团队攻读硕士和博士研究生。