姓名：谢幼华 博士
职称：研究员
E-MAIL: yhxie@fudan.edu.cn


研究方向
1.乙肝病毒的感染和致病机制及抗病毒药物研究
2.肝癌发生发展的机理和治疗方法的研究
重要学术贡献
1.建立乙肝病毒载体系统，成为示踪乙肝病毒感染细胞的技术平台，同时也具有开发为肝脏靶向基因治疗载体的潜能（专利ZL5.0）；
2.设计和发展了一种基于短肽的抗HBV感染的进入抑制剂（专利ZL7.4）；
3.揭示细胞因子IL-21和IL-33在乙肝病毒慢性感染清除中的关键作用，以及其在慢性乙肝治疗中的潜在用途；
4.揭示了以PROX1为核心的肝癌转移新机制，藉此提出了治疗肝癌转移的新策略。
荣誉与获奖
2014 教育部自然科学一等奖（排名第4）
2012 上海市公共卫生优秀学科带头人
国内外学术团体任职
2017-今 中华医学会微生物学与免疫学分会委员
2014-今 中华医学会上海市医学会病毒专业委员会副主任委员
受邀审稿学术刊物
2010-今 为Hepatology、Frontiers in Immunology、PLoS ONE、Scientific Reports、Virol J、J Med Virol、Cancer Letters等刊物审稿。
校内学术机构任职
基础医学院教授委员会/委员
受邀学术报告
1.2017.3.16-19 乙肝病毒进入抑制剂的研究，第二届全国病毒进入（入侵）及进入抑制剂研讨会，云南大理
2.2017.10.28-29 Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as potent regulators of viral clearance with therapeutic potential，京沪病毒学论坛，北京
3.2015.11 New cell and animal model for HBV，中华医学会2015年医学病毒学大会，上海
发表论文
1.Shen ZL, Yang HJ, Yang SS, Wang W, Cui XX, Zhou X, Liu W, Pan SK, Liu YF, Zhang JQ, Zhang JM, Xie YH*, Liu J*. Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance. Nature Communications, 2017 Dec 14;8(1):2119.
2.Shen YX, Zhang JQ*, Hao WJ, Wang T, Liu J, Xie YH*, Xu SH*, Liu HL. Copolymer micelles function as pH-responsive nanocarriers to enhance the cytotoxicity of a HER2 aptamer in HER2-positive breast cancer cells. International Journal of Nanomedicine, accepted
3.Cui XX, Yang X, Wang HJ, Rong XY, Jing S, Xie YH*, Huang DF*, Zhao C*. Luteolin-7-O-glucoside present in lettuce extracts inhibits hepatitis B surface antigen production and viral replication by human hepatoma cells in vitro. Frontiers in Microbiology, accepted
4.Wang S, Tang L, Lin J, Shen Z, Yao Y, Wang W, Tao S, Gu C, Ma J*, Xie Y*, Liu Y*. ABCB5 promotes melanoma metastasis through enhancing NF-κB p65 protein stability. Biochem Biophys Res Commun. 2017 Oct 7;492(1):18-26.
5.Liu YF, Zhang YL, Wang SH, Dong QZ, Shen ZL, Wang W, Tao S, Gu CJ, Liu J*, Xie YH*, Qin LX*. Prospero-related homeobox 1 drives angiogenesis of hepatocellular carcinoma through selectively activating IL-8 expression. Hepatology. 2017 Dec; 66(6):1894-1909
6.Lin JY, Gu CJ, Shen ZL, Liu YF, Wang W, Tao S, Cui XX, Liu J*, Xie YH*. Hepatocyte Nuclear Factor 1α Downregulates HBV Gene Expression and Replication by Activating the NF-?B Signaling Pathway. PLOS ONE. 2017 Mar 20;12(3):e**.
7.Ma JX, Wu KF, Bai WY, Cui XX, Chen Y, Xie YH*, Xie YH*. Synergistic Cytotoxicity of Lenalidomide and Dexamethasone in Mantle Cell Lymphoma via Cereblon-dependent Targeting of the IL-6/STAT3/PI3K Axis. EBioMedicine. 2017 Jun;20:70-78.
8.Zhang SJ, Yu N, Wang LF, Liu YF, Kong YY, Liu J, Xie YH*. Prox1 represses IL-2 gene expression by interacting with NFAT2. Oncotarget. 2017 Apr 20. doi: 10.18632/oncotarget.17278.
9.Han Y, Gu L, Liu J, Li X, Wang M, Gong Q, Yu D, Yang Z, Zhang D, Yang H, Shen Z, Zhu H, Xie Y*, Zhang X*. Association of mutations in TLR2 signaling genes with fulminant form of hepatitis B related acute liver failure. J Infect Dis. 2017 Apr 15;215(8):1221-1230.
10.Chen R, Zhao X, Wang Y, Xie Y*, Liu J*. Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G. Sci Rep. 2017 Jan 18;7:40783. doi: 10.1038/srep40783.
11.Shen Z, Rao Y, Tao S, Luo M, Ming L, Liu J, Pan S, Xie Y*. Unimpaired immunogenicity of yeast-expressed hepatitis B surface antigen stored at elevated temperatures. Acta Biochim Biophys Sin. 2016 Dec;48(12):1094-1100.
12.Shen ZL, Liu YF, Luo MJ, Wang W, Liu J, Liu W, Pan SK, Xie YH*. Nuclear factor Y regulates ancient budgerigar hepadnavirus core promoter activity. Biochem Biophys Res Commun. 2016 Sep 16;478(2):825-30.
13.Ye XL, Zhou M, He YG, Wan YM, Bai WY, Tao S, Ren YP, Zhang XX, Xu JQ, Liu J, Zhang JQ*, Hu KH*, Xie YH*. Efficient Inhibition of Hepatitis B Virus Infection by a preS1-binding Peptide. Sci Rep. 2016 Jul 7;6:29391.
14.Bai WY, Cui XX, Chen RD, Tao S, Hong R, Zhang JM, Zhang JQ, Wang YX, Xie YH*, Liu J*. Re-Designed Recombinant Hepatitis B Virus Vectors Enable Efficient Delivery of Versatile Cargo Genes to Hepatocytes with Improved Safety. Viruses. 2016 May 10;8(5). E129.
15.Bai WY, Cui XX, Xie YH*, Liu J*. Engineering Hepadnaviruses as Reporter-Expressing Vectors: Recent Progress and Future Perspectives. Viruses. 2016 May 10;8(5). E125. Review.
16.Yan L, Qu S, Liu G, Liu L, Yu Y, Ding G, Zhao Y, Li Y, Xie Y*, Zhang J*, Qu D*. Comparative Transcriptomic Analysis of Primary Duck Hepatocytes Provides Insight into Differential Susceptibility to DHBV Infection. PLoS One. 2016 Feb 22;11(2):e**.
17.Liu Y, Ye X, Zhang JB, Ouyang H, Shen Z, Wu Y, Wang W, Wu J, Tao S, Yang X, Qiao K, Zhang J, Liu J*, Fu Q*, Xie Y*. PROX1 promotes hepatocellular carcinoma proliferation and sorafenib resistance by enhancing β-catenin expression and nuclear translocation. Oncogene. 2015 Oct 29;34(44):5524-35.
18.He Y, Ye X, Tiollais P, Zhang J, Zhang J, Liu J, Xie Y*. Selection of HBV preS1-binding penta-peptides by phage display. Acta Biochim Biophys Sin. 2014 Aug;46(8):691-8.
19.Zhao X, Wu YX, Duan JL, Ma YC, Shen ZL, Wei LL, Cui XX, Zhang JQ, Xie YH*, Liu J*. Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS. J Proteome Res. 2014, Dec 5; 13:5391-5402
20.Wei LL, Shen ZL, Zhao X, Wu YX, Liu W, Zhang JQ, Xie YH*, Liu J*. A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein. Arch Virol. 2014, 159:2731-5
21.Lv¬¬¬ T, Liu YF, Zhang JP, Xu L, Zhu Y, Yin HK, An HM, Chen L, Xie YH*, Lin ZM*. Impact of an altered PROX1 expression on clinicopathology, prognosis and progression in renal cell carcinoma. PLOS One. 2014 May 5;9(5):e95996.
22.Ouyang HF, Qin Y, Liu YF, Xie YH*, Liu J*. Prox1 directly interacts with LSD1 and recruits the LSD1/NuRD complex to epigenetically co-repress CYP7A1 transcription. PLOS One. 2013, 8(4):e62192
23.Hong R, Bai WY, Zhai JW, Liu W, Li XY, Zhang JM, Cui XX, Zhao X, Ye XL, Deng Q, Tiollais P, Wen YM, Liu J*, Xie YH*. Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes. J Virol. 2013, 87(12):6615-24
24.Liu YF, Zhang JB, Qin Y, Wang W, Wei LL, Teng YG, Guo L, Zhang B, Lin ZH, Liu J, Ren ZG*, Ye QH*, Xie YH*. PROX1 promotes hepatocellular carcinoma metastasis via up-regulating hypoxia-inducible factor 1a expression and protein stability. Hepatology. 2013; 58(2):692-705.
25.Qin Y, Ouyang HF, Liu J, Xie YH*. Proteome identification of proteins interacting with histone methyltransferase SET8. Acta Biochim Biophys Sin. 2013, 45(4):303-8
26.Liu W, Pan SK, Yang HJ, Bai WY, Shen ZL, Liu J*, Xie YH*. The First Full-length Endogenous Hepadnaviruses: Identification and Analysis. J Virol. 2012, 86(17):9510-3
27.Liu W, Liu YF, Liu J, Zhai JW, Xie YH*. Evidence for inter- and intra-clade recombinations in rabies virus. Infect Genets Evol 2011, 11:1906-1912
28.Xie YH*, Hong R, Liu W, Liu J, Zhai JW. Development of novel therapeutics for chronic hepatitis B. Virol Sin. 2010, 25(4):294-300
29.Xie YH*, Zhai JW, Deng Q, Tiollais P, Wang Y, Zhao MJ. Entry of hepatitis B virus: mechanism and new therapeutic target. Pathol Biol. 2010, 58(4):301-307
30.Tian X, Zhao C, Zhu HG, She WM, Zhang JM, Liu J, Li LJ, Zheng SS, Wen YM, Xie YH*. Hepatitis B virus (HBV) surface antigen interacts with and promotes cyclophilin a secretion: possible link to pathogenesis of HBV infection. J Virol. 2010, 84(7):3373-3381
31.Liu W, Zhai JW, Liu J, Xie YH*. Identification of natural recombination in Duck Hepatitis B Virus. Virus Res. 2010, 149:245-251
32.Liu W, Zhai JW, Liu J, Xie YH*. Identification of recombination between subgenotypes IA and IB of hepatitis A virus. Virus Genes 2010, 40(2):222-224
33.Qin J, Zhai JW, Hong R, Shan SF, Kong YY, Wen YM, Wang Y, Liu J, Xie YH*. Prospero-related homeobox protein (Prox1) inhibits hepatitis B virus replication through repressing multiple cis regulatory elements. J Gen Virol. 2009, 90(Pt 5):1246-1255
34.Zhai JW, Gao DM, Liu W, Hong R, Qin Y, Ouyang HF, Kong YY, Wang Y, Xie YH*, Liu J*. Identification and characterization of a novel isoform of murine interferon regulatory factor 3. Biochem Biophys Res Commun. 2008, 377(2):384-388
35.Shan SF, Wang LF, Zhai JW, Qin Y, Ouyang HF, Kong YY, Liu J, Wang Y, Xie YH*. Modulation of transcriptional corepressor activity of Prospero-related homeobox protein (Prox1) by SUMO modification. FEBS lett. 2008, 582(27):3723-3728
36.Wang LF, Zhu JM, Shan SF, Qin Yi, Kong YY, Liu J, Wang Y, Xie YH*. Repression of interferon gamma (IFN-g) expression in T cells by Prospero-related Homeobox (Prox1) protein. Cell Res. 2008, 18(9):911-920
37.Deng Q, Zhai JW, Michel ML, Zhang J, Kong YY, Qin J, Zhang XX, Tiollais P, Wang Y*, Xie YH*. Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site. J Virol. 2007, 81(8):4244-4254
38.Tian XC, Zhao C, Ren J, Ma ZM, Xie YH*, Wen YM*. Gene expression profiles of a hepatitis B small surface antigen secreting cell line revealed up-regulation of lymphoid enhancer-binding factor 1. J Gen Virol. 2007, 88(Pt 11):2966-2976
39.Gao DM, Wang LF, Liu J, Kong YY, Wang Y, Xie YH*. Expression of mouse liver receptor homologue 1 in embryonic stem cells is directed by a novel promoter. FEBS lett. 2006, 580(7):1702-1708
40.Deng Q, Kong YY, Xie YH*, Wang Y*. Screening for PreS specific binding ligands with a phage displayed peptides library. World J Gastroenterol. 2005, 11(26):4018-4023
41.Deng Q, Kong YY, Xie YH*, Wang Y*. Expression and purification of the complete PreS region of hepatitis B Virus. World J Gastroenterol. 2005, 11(20):3060-3064
42.Qin J, Gao DM, Jiang QF, Zhou Q, Kong YY, Wang Y*, Xie YH*. Prospero-related homeobox (Prox1) is a corepressor of human liver receptor homolog-1 and suppresses the transcription of the cholesterol 7-alpha-hydroxylase gene. Mol Endocrinol. 2004, 18(10):2424-2439
43.Xu PL, Liu YQ, Shan SF, Kong YY, Zhou Q, Li M, Ding JP, Xie YH*, Wang Y*. Molecular mechanism for the potentiation of the transcriptional activity of human liver receptor homologue 1 by steroid receptor coactivator-1. Mol Endocrinol. 2004, 18(8):1887-1905
44.Cai YN, Zhou Q, Kong YY, Li M, Viollet B, Xie YH*, Wang Y*. LRH-1/hB1F and HNF1 synergistically up-regulate hepatitis B virus gene transcription and DNA replication. Cell Res. 2003, 13(6):451-458
45.Xu PL, Shan SF, Kong YY, Xie YH*, Wang Y*. Characterization of a strong repression domain in the hinge region of orphan nuclear receptor LRH-1/hB1F. Acta Biochimica et Biophysica Sinica. 2003, 35(10):909-916
46.Xu PL, Kong YY, Xie YH*, Wang Y*. Corepressor SMRT specifically represses the transcriptional activity of orphan nuclear receptor LRH-1/hB1F. Acta Biochimica et Biophysica Sinica. 2003, 35(10):897-903
学术专著
1.复旦大学出版社，《医学微生物学》，2016，副主编
2.大百科全书第三版，现代医学分册，《病原生物学》，副主编
申请或授权专利
授权专利
1.谢幼华、何永刚、汪垣、邓强、刘晶、周佳海、董琦蕾。一种抑制人乙型肝炎病毒感染的短肽及其应用。申请日：2011.5.12；授权公告日：2014.9.3；ZL7.4
2.谢幼华、洪冉、翟建伟、刘晶、张继明、李新艳、刘伟。一种高效复制的人乙型肝炎病毒重组载体及其应用；申请日：2011.2.16；授权公告日：2014.4.2；ZL5.0
申请专利
1.谢幼华、刘晶、沈忠良、潘少坤。白介素33（IL-33）在制备抗乙型肝炎病毒药物制剂中的用途。申请日：2017年1月18日；7.X
2.谢幼华、刘晶、沈忠良、潘少坤。白介素21（IL-21）在制备抗乙型肝炎病毒药物制剂中的用途。申请日：2017年1月18日；8.4
3.谢幼华、谷陈建、刘晶、陶帅、潘少坤。一种评价人乙型肝炎病毒X蛋白结合分子的方法。申请日：2016年12月27日；8.5
4.谢幼华、刘晶、沈忠良、杨慧娟、潘少坤。乙型肝炎病毒（HBV）慢性化小鼠模型的建立方法及用途。申请日：2016年7月31日；3.1
5.刘晶、谢幼华、柏伟娅、崔晓娴。一种人乙型肝炎病毒重组载体及其应用。申请日：2015年3月25日；8.4
6.谢幼华、刘晶、魏丽丽、沈忠良、潘少坤。一种人乙型肝炎病毒X蛋白的单克隆抗体及用途。申请日：2015年3月25日；6.7