卢山 教授 210023





办公电话：
邮政编码： 210023

联系地址： 生科院A210
电子邮件： shanlu@nju.edu.cn








个人简介
研究方向
科研成果





个人简介
工作经历：

2009.05- 南京大学生命科学学院，博士研究生导师

2007.12-南京大学生命科学学院，教授
2006.09-2007.12南京大学生命科学学院，副教授
2002.12-2006.04美国康奈尔大学，博士后(导师：Dr. Li Li)
2001.09-2002.11德国马普化学生态所，博士后(导师：Dr. Jonathan Gershenzon)
1998.12-2001.09中科院上海植物生理生态研究所，博士后(导师：陈晓亚研究员)
1998.02-1998.10美国康涅狄克大学，访问****(导师：Dr. Charles Yarish)
1997.02-1998.12中科院海洋所，博士后(导师：曾呈奎院士、费修绠研究员)

学习经历：
1994.09-1997.01南京大学生物学系，植物学专业，博士研究生，导师：刘志礼教授
1991.07-1994.09南京大学生物学系，植物学专业，硕士研究生，导师：陈晓亚副教授
1987.09-1991.07南京大学生物学系，植物学专业，本科生

学术兼职

学会任职：
中国海洋湖沼学会藻类学分会: 副理事长 (2007-)
中国植物生理与植物分子生物学学会：理事(2014-)
江苏省植物生理学会：副理事长(2015-)
American Association for the Advancement of Science: 会员 (2003-)
American Society of Plant Biologists: 会员 (2003-)

期刊编委：
Journal of Integrative Plant Biology
植物生理学报

审稿人：
Nature Plants, Nature Communications, Plant Cell, Molecular Plant, Plant Physiology, Plant Journal, Plant Biotechnology Journal, Metabolic Engineering, New Phytologist, Trends in Plant Science, Plant Cell Physiology, Plant Cell & Environment, Journal of Integrative Plant Biology等





研究方向
本实验室利用分子生物学手段研究与植物叶绿体发育相关的代谢过程及其调控机制。

牻牛儿基牻牛儿基二磷酸(GGPP)是植物萜类代谢途径中的一个关键分支点。植物以GGPP为底物，合成大约2万种天然产物，其中包括激素(赤霉素、脱落酸、独脚金内酯)、色素(类胡萝卜素和叶绿素的植基侧链)、维生素(来自胡萝卜素的维生素A和维生素E的植基侧链)，以及参与植物对病原微生物防御机制的二萜植保素。这些代谢分支共同竞争GGPP，构成了牵一发而动全身的复杂网络。GGPP的合成及其在下游分支间的分配机制影响了植物的生长发育和逆境适应能力，也影响着农产品的营养品质和风味特色，以及园艺植物的观赏价值。

本实验室近年来围绕这一核心问题开展了一系列研究工作。主要进展包括：

1. 解析了水稻中GGPP保障叶绿素代谢的调控机制(Zhou et al., 2017, PNAS)；

[类囊体膜上LIL3作为锚蛋白结合GGPPS及其招募蛋白GRP，将代谢流引入叶绿素合成途径(经叶绿素合酶CHLG催化)。我们发现并解析了GGPPS/GRP异源二聚体和GGPPS同源二聚体，并鉴定了其酶促动力学特性。]

2. 解析了锌指蛋白ORANGE在子叶中通过转录因子TCP14对叶绿素合成和叶绿体发育的抑制作用(Sun et al., 2019, Plant Cell)；
[TCP14在细胞核内诱导ELIPs基因从而促进叶绿素合成与叶绿体发育。在黑暗中OR蛋白位于核里并抑制TCP14。种子破土而出后，核里的OR蛋白逐渐减少，从而推动叶绿素合成与叶绿体发育。而转入叶绿体的OR则促进类胡萝卜素合成来形成对叶绿体新的保护]

3. 解析了辣椒果实中类胡萝卜素合成的部分代谢流调控机制(Wang et al., 2018, 219, J Agric Food Chem)；

4. 以紫菜为主要材料，系统研究了红藻的类胡萝卜素代谢途径，初步揭示了这一重要代谢途径在植物中的早期演化(Yang et al., 2014, J Intergr Plant Biol; 2015, J Appl Phycol; Cao et al., 2017, Mar Drugs; Deng et al., 2020, J Agric Food Chem)；并建立了基于蓝细菌的酶活鉴定系统和合成生物学光驱动底盘(Cao et al., 2020, Plant Biotechnol J)。














获奖情况


科研成果

部分研究论文(*为通讯作者)：


Kong M-J, Huang N, Chen S-M, Liang H-Y, Liu X-Y, Zhuang Z, Lu S*. 2020. The nuclear localization of the DnaJ-like zinc finger domain-containing protein EDA3 affects seed development in Arabidopsis thaliana. International Journal of Molecular Sciences21: 7979.
Huang X-Q, Wang L-J, Kong M-J, Huang N, Liu X-Y, Liang H-Y, Zhang J-X, Lu S*. At3g53630 encodes a GUN1-interacting protein under norflurazon treatment. Protoplasma(https://doi.org/10.1007/s00709-020-01578-x).
Deng Y-Y, Wang Q, Cao T-J, Zheng H, Ge Z-H, Yang L-E*, Lu S*. 2020. Cloning and functional characterization of the bona fidegeranylgeranyl diphosphate synthase from the red algal seaweed Bangia fuscopurpurea. Algal Research48: 101935.
Deng Y-Y, Cheng L, Wang Q, Ge Z-H, Zheng H, Cao T-J, Lu Q-Q, Yang L-E*, Lu S*. 2020. Functional characterization of lycopene cyclases illustrates the metabolic pathway toward lutein in red algal seaweeds. Journal of Agricultural and Food Chemistry68: 1354-1363
Cao T-J, Wang L-J, Huang X-Q, Deng Y-Y, Yang L-E*, Lu S*. 2020. Manipulation of Synechocystissp. PCC 6803 as a platform for functional identification of genes involved in carotenoid metabolism. Plant Biotechnology Journal18: 605-607
Sun T, Zhou F, Huang X-Q, Chen W-C, Kong M-J, Zhou C-F, Zhuang Z, Li L, Lu S*. 2019. ORANGE represses chloroplast biogenesis in etiolated Arabidopsis cotyledons via interaction with TCP14. Plant Cell31: 2996-3014 (文章链接)
Wang Q, Cao T-J, Zheng H, Zhou C-F, Wang Z, Wang R*, Lu S*. 2019. Manipulation of carotenoid metabolic flux by lycopene cyclization in ripening red pepper (Capsicum annuumvar. conoides) fruits. Journal of Agricultural and Food Chemistry67: 4300-4310.
Wang Q, Huang X-Q, Cao T-J. Zhuang Z, Wang R*, Lu S*. 2018. Heteromeric geranylgeranyl diphosphate synthase contrubutes to carotenoid biosynthesis in ripening fruits of red pepper (Capsicum annuumvar. conoides). Journal of Agricultural and Food Chemistry66: 11691-11700.
Huang X-Q, Zhao L, Rui J-D, Zhou C-F, Zhuang Z, Lu S*. 2017. At5g19540encodes a novel protein that affects pigment metabolism and chloroplast development in Arabidopsis thaliana. Frontiers in Plant Science8: 2140.
Zhou F, Wang C-Y, Gutensohn M, Jiang L, Zhang P, Zhang D, Dudareva N*, Lu S*. 2017. A recruiting protein of geranylgeranyl diphosphate synthase controls metabolic flux toward chlorophyll biosynthesis in rice. Proc. Natl. Acad. Sci. USA114: 6860-6865.(文章链接)
Cao T-J, Huang X-Q, Qu Y-Y, Zhuang Z, Deng Y-Y*, Lu S*. 2017. Cloning and functional characterization of a lycopene β-cyclase from macrophytic red alga Bangia fuscopurpurea. Marine Drugs15: 116.
Wang Y-W, Chen S-M, Wang W-J, Huang X-Q, Zhou C-F, Zhuang Z, Lu S*. 2016. The DnaJ-like zinc finger domain protein PSA2 affects light acclimation and chloroplast development in Arabidiopsis thaliana. Frontiers in Plant Science7: 360
Sun T-H, Zhou F, Liu C-J, Zhuang Z, Lu S*. 2016. The DnaJ-like zinc finger domain protein ORANGElocalizes to the nucleus in etiolated cotyledons of Arabidopsis thaliana. Protoplasma253: 1599-1604
Zhou F, Sun T-H, Zhao L, Pan X-W, Lu S*. 2015. The bZIP transcription factor HY5 interacts with the promoter of the monoterpene synthase gene QH6in modulating its rhythmicexpression. Frontiers in Plant Science6: 304
Yang L-E, Huang X-Q, Lu Q-Q, Zhu J-Y, Lu S*. 2015. Cloning and characterization of the geranylgeranyl diphosphate synthase (GGPS) responsible for carotenoid biosynthesis in Pyropia umbilicalis. Journal of Applied Phycology28: 671-678
Yang L-E, Huang X-Q, Hang Y, Deng Y-Y, Lu Q-Q, Lu S*. 2014. The P450-type carotene hydroxylase PuCHY1 from Porphyrasuggests the evolution of carotenoid metabolism in red algae. Journal of Integrative Plant Biology56: 902-915.
Yang L-E, Jin Q-P, Xiao Y, Xu P, Lu S*. 2013. Improved methods for basic molecular manipulation of the red alga Porphyra umbilicalis(Rhodophyta: Bangiales). Journal of Applied Phycology25: 245-252.
Lu S*, Yarish C. 2011. Interactionof photoperiod and temperature in the development of conchocelis of Porphyra purpurea(Rhodophyta: Bangiales). Journal of Applied Phycology23: 89-96.
Sun T-H, Liu C-Q, Hui Y-Y, Wu W-K, Zhou Z-G, Lu S*.2010. Coordinated regulationof gene expression for carotenoid metabolism in Chlamydomonas reinhardtii.Journal of Integrative Plant Biology52: 868-878.
Lu S, Van Eck J, Zhou XJ, Lopez AB, O'Halloran DM, Cosman KM, Conlin BJ, Paolillo DJ, Garvin DF, Vrebalov J, Kochian LV, Kuepper H, Earle ED, Cao J, Li L*. 2006. The cauliflower Orgene encodes a cysteine-rich zinc finger domain-containing protein that mediates high-levels of beta-carotene accumulation. Plant Cell18: 3594-3605.
Lu S, Xu R, Jia JW, Pang J, Matsuda SPT, Chen XY*. 2002. Cloning and functional characterization of a beta-pinene synthase from Artemisia annuathat shows a circadian pattern of expression. Plant Physiology130: 477-486
Lu S*, Zhang XN, Li PY, Liu ZL, Xia ZH. 1996. A salt-induced chloroplast protein from Dunaliella salina(Chlorophyta). Journal of Phycology32: 983-986


专著章节：


Lu S. 2015. Plant Metabolic Network. In: Plant Metabolomics (Ed: Qi X-Q, Wang Y-L, Chen, X-Y). Springer. pp. 195-212.
Gantt E, Berg GM, Bhattacharya D, Blouin NA, Brodie JA, Chan CX, Collen J, Cunningham FXJr, Gross J, Grossman AR, Karpowicz S, Kitade Y, Klein AS, Levine IA, Lin SJ, Lu S, Lynch M, Minocha SC, Muller K, Neefus CD, De Oliveira MC, Rymarquis L, Smith A, Stiller JW, Wu WK, Yarish C, Zhuang YY, Brawley SH. 2010. Porphyra: complex life history in a harsh environment. P. umbilicalis, an intertidal red alga for genomic analysis. In Cellular Origin, Life in Extreme Habitats and Astrobiology. Vol. 13. Red Algaein the Genomic Age. (Sechbach J, Chapman DJ eds.). Springer. pp. 130-148.
Van Eck J, Zhou XJ, Lu S, Li L. 2009. Modulation of carotenoid accumulation in transgenic potato by inducing chromoplast formation with enhanced sink strength. In: Metabolic Engineering of Plant Secondary Pathways (Ed.: Walker J). Humana Press. pp. 77-93.
卢山，陈晓亚. 2012. 植物次生代谢。植物生理与分子生物学(第四版)(陈晓亚、薛红卫主编)。高等教育出版社. pp. 369-389
卢山. 2011. 植物代谢网络。植物代谢组学(漆小泉、王玉兰、陈晓亚主编)。化学工业出版社. pp. 184-202
陈晓亚，卢山. 2007. 植物次生代谢。植物生理与分子生物学(第三版)(陈晓亚、汤章城主编)。高等教育出版社. pp. 329-356
Lu S, Chen XY. 2005. Terpenoid metabolism in cotton and qiaohao (Artemisia annua). In: Advances in Plant Physiology (Ed.: Hemantaranjan A), Vol. 8. Pawan Kumar Scientific Publishers (India). pp. 265-292.
费修绠, 王素娟, 卢山. 1998. 红藻类细胞工程育种和育苗. 海洋生物技术. 山东科技出版社. pp. 7-26.