兰文智 教授 博士生导师





办公电话：
邮政编码： 210093

联系地址：
电子邮件： lanw@nju.edu.cn








个人简介
研究方向
科研成果





个人简介
2003－2006年：加拿大皇后大学（Queen’s University），博士后。
2006－2011年：美国加州大学伯克利分校（University of California at Berkeley），博士后、Associate Specialist。

2011年10月－今：南京大学生命科学学院教授。

2012年－今：南京大学博士生导师。

学术兼职



研究方向
研究方向和兴趣：
营养分配和植物生长发育；植物营养稳态与逆境胁迫
研究内容：

生命活动依赖于物质的有效分配。植物利用各种膜（质膜和内膜）定位的转运体蛋白将营养物质(养分)运送到特定的细胞空间，优化有限养分的利用，保障正常/逆境条件下生长发育的需要。本课题组主要利用分子生物学、生物物理学、组织化学、蛋白荧光标记和分子遗传学等方法，探讨植物协调物质分配的分子机理；侧重于鉴定关键膜系统蛋白组分及其信号转导过程，为分子培育优质作物提供新基因和新理论。


1. 植物调节细胞内-外的离子分配。植物细胞利用质膜定位的转运体蛋白（Antiportors, Symporters, Uniporters, Channels）和质子泵（提供质子梯度作为推动力）与外界环境进行物质交换，并可根据环境的变化，调节转运活性（信号转导）以维持胞内物质的稳态。我们主要鉴定转运特定离子的转运体基因及其调控机制。


2. 胞质-细胞器的矿物质交换。矿质离子进入胞内后，除了少量以满足胞质活动，大部分进入细胞器，特别是液泡和叶绿体。由于液泡和叶绿体是植物特有的细胞器，我们侧重于研究这两种细胞器如何从胞质吸收矿质离子。

3. 转运体蛋白膜丰度的调控。根据生长阶段或环境的变化，植物有序地调节膜蛋白的胞内运输，包括上膜（如高尔基体-反面高尔基网络TGN-液泡膜/质膜）和下膜（质膜-液泡膜），改变转运体蛋白在膜系统的分布和含量（丰度）。


招生招聘：2015.09-2020.06期间，培养出9位博士生(张弛，尉静玲，张斌，王愿，刘金隆，栾明达，荆彦平，张丰，蔡国华）。热忱欢迎有志于科研的同学加入我们的课题组!




获奖情况
1. 第二届江苏省****。
2. 2017年度“南京大学优秀博士论文”和2017年度“江苏省优秀博士论文优秀博士论文”指导教师。
3. 湖北省2006年度科学技术三等奖(编号2006Z-033-3-015-012)。


科研成果
Selected：(*，第一作者；#，通讯作者）


Chu M*, Wang Y, Mu B, Ge H, Zhang C, Zhao F, Fu A, Luan S, Li L, Lan W#. An ICln Homolog Contributes to Osmotic and Low-Nitrate Tolerance by Enhancing Nitrate Accumulation in Arabidopsis. Plant Cell Environ.2021 Jan 25. doi: 10.1111/pce.14005.
全文链接：https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14005
PCE公众号报道：https://mp.weixin.qq.com/s/0jBsTAdygFasWlZ0moZjPA

Chu M*, Chen P, Meng S, Xu P,Lan W#.The Arabidopsis phosphatase PP2C49 negatively regulates salt tolerance through inhibition of AtHKT1;1.J Integr Plant Biol.2020 Sep 2. doi: 10.1111/jipb.13008. (第一作者：储谟立博士生)。
全 文 链接：https://onlinelibrary.wiley.com/doi/10.1111/jipb.13008
公众号解读：https://mp.weixin.qq.com/s/6rY95FTu0HcEceveL1LPeA


Zhi Zhang*, Bin Zhang*, Baicong Mu, Xiaojiang Zheng, Fugeng Zhao, Wenzhi Lan#, Aigen Fu#, Sheng Luan#. A Thylakoid Membrane Protein Functions Synergistically with GUN5 in Chlorophyll Biosynthesis. Plant Communications, Vol. 1, Issue 5, 100094, September 14, 2020.(第一作者：张弛博士/张斌博士）全文链接：https://www.cell.com/plant-communications/fulltext/S2590-3462(20)30118-8
新闻解读：https://mp.weixin.qq.com/s/Y-uDcrRK81zEp6WiyF8UCw

Jing Y*, Zheng X*, Zhang D, Shen N, Wang Y, Yang L, Fu A, Shi J, Zhao F, Lan W#, Luan S#. Danger-Associated Peptides Interact with PIN-Dependent Local Auxin Distribution to Inhibit Root Growth in Arabidopsis.Plant Cell. 2019 Aug;31(8):1767-1787. doi: 10.1105/tpc.18.00757.(第一作者：荆彦平博士生/郑小江博士后)。
全 文 链接：http://www.plantcell.org/content/31/8/1767.long
公众号解读：https://news.nju.edu.cn/xsdt/**/i94083.html

Luan M*, Lan W#. Escape routes for vacuolar phosphate. Nat Plants.2019 Jan;5(1):9-10. doi: 10.1038/s41477-018-0343-2. (Invited Comment)(第一作者：栾明达博士生)。
全文链接：https://www.nature.com/articles/s41477-018-0343-2


Zhang F*, Yan X, Han X, Tang R, Chu M, Yang Y, Yang YH, Zhao F, Fu A, Luan S#, Lan W#. A Defective Vacuolar Proton Pump Enhances Aluminum Tolerance by Reducing Vacuole Sequestration of Organic Acids. Plant Physiol. 2019 Oct;181(2):743-761. doi: 10.1104/pp.19.00626 (第一作者：张丰博士生)。
全文链接：https://doi.org/10.1104/pp.19.00626
Plant Physiol 评论：http://www.plantphysiol.org/content/181/2/381
公众号报道：https://mp.weixin.qq.com/s/v7c7NAcZ72OoWnVULK-_fQ

Luan M*, Zhao F, Han X, Sun G, Yang Y, Liu J, Shi J, Fu A, Lan W#, Luan S#. Vacuolar phosphate transporters contribute to systemic phosphate homeostasis vital for reproductive development in Arabidopsis.Plant Physiol.2019 Feb;179(2):640-655. doi: 10.1104/pp.18.01424.(第一作者：栾明达博士生)。
全文链接：http://www.plantphysiol.org/content/179/2/640.long
公众号报道：https://mp.weixin.qq.com/s/XPpg1OUtz9t3ENrXyMYCEA

Zhang B*, Zhang C*, Liu C, Jing Y, Wang Y, Jin L, Yang L, Fu A, Shi J, Zhao F, Lan W#, Luan S#. Inner Envelope CHLOROPLAST MANGANESE TRANSPORTER 1 Supports Manganese Homeostasis and Phototrophic Growth in Arabidopsis. Mol Plant.2018 Jul 2;11(7):943-954. doi: 10.1016/j.molp.2018.04.007. (第一作者：张斌博士生/张弛博士生）Mol Plant 封面文章。
全文链接：https://www.sciencedirect.com/science/article/pii/S**01576?via%3Dihub
Mol Plant评论：https://www.sciencedirect.com/science/article/pii/S**02405
公众号报道：https://mp.weixin.qq.com/s/eZ7-kiYe56MeVyfeqnjLqA

Zheng X*, Kang S*, Jing Y, Ren Z, Li L, Zhou JM, Berkowitz G, Shi J, Fu A, Lan W, Zhao F#, Luan S#. Danger-Associated Peptides Close Stomata by OST1-Independent Activation of Anion Channels in Guard Cells. Plant Cell. 2018 May;30(5):1132-1146. doi: 10.1105/tpc.17.00701. Epub 2018 Apr 30. (第一作者：郑小江博士后/韩国KAIST Kang博士后)。
全文链接：http://www.plantcell.org/content/30/5/1132.long

Luan M*, Liu J*, Liu Y, Han X, Sun G, Lan W#, Luan S#. Vacuolar Phosphate Transporter 1 (VPT1) Affects Arsenate Tolerance by Regulating Phosphate Homeostasis in Arabidopsis. Plant Cell Physiol.2018 Feb 6. doi: 10.1093/pcp/pcy025.(第一作者：栾明达博士生/刘金隆博士生)。

Wang Y*, Yang L*, Tang Y, Tang R, Jing Y, Zhang C, Zhang B, Li X, Cui Y, Zhang C, Shi J, Zhao F, Lan W#, Luan S#. Arabidopsis choline transporter-like 1 (CTL1) regulates secretory trafficking of auxin transporters to control seedling growth. PLoS Biol.2017 Dec 28;15(12):e**. (第一作者：王愿博士生/杨磊副研究员)。
全文链接：https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.**
PLoS Biol评论：https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.**

Zhang C*, Li H*, Wang J, Zhang B, Wang W, Lin H, Luan S, Gao J#, Lan W#. The Rice High-Affinity K+ Transporter OsHKT2;4 Mediates Mg2+ Homeostasis under High-Mg2+ Conditions in Transgenic Arabidopsis. Front Plant Sci. 2017, 24;8:1823. (第一作者：张弛博士生/李合娟硕士生）
全文链接：https://www.frontiersin.org/articles/10.3389/fpls.2017.01823/full

Luan M*, Tang RJ, Tang Y, Tian W, Hou C, Zhao F, Lan W#, Luan S#. Transport and homeostasis of potassium and phosphate: limiting factors for sustainable crop production. J Exp Bot.2017 Jun 1;68(12):3091-3105.(第一作者：栾明达博士生)。

Liu J*, Yang L, Luan M, Wang Y, Zhang C, Zhang B, Shi J, Zhao FG#, Lan W#, Luan S# (2015) A vacuolar phosphate transporter essential for phosphate homeostasis in Arabidopsis. Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):E6571-8.(第一作者：刘金隆博士生)。
全文链接：https://www.pnas.org/content/112/47/E6571.long

Chen J, Yu F#, Liu Y, Du C, Li X, Zhu S, Wang X, Lan W, Rodriguez PL, Liu X, Li D, Chen L#, Luan S#. FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis. Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):E5519-27. doi: 10.1073/pnas..

Zhou L*, Lan W*, Chen B, Fang W, Luan S#. A calcium sensor-regulated protein kinase, CALCINEURIN B-LIKE PROTEIN-INTERACTING PROTEIN KINASE19, is required for pollen tube growth and polarity. Plant Physiol., 2015 Apr;167(4):1351-60.

Zhou L*, Lan W*, Jiang Y, Fang W, Luan S#(2014) A calcium-dependent protein kinase interacts with and activates a calcium channel to regulate pollen tube growth. Mol Plant7(2):369-76.

Lee SC*, Lim CW*, Lan W*, He K, Luan S#. (2013). ABA Signaling in Guard Cells Entails a Dynamic Protein-Protein Interaction Relay from the PYL-RCAR Family Receptors to Ion Channels. Mol Plant, 6(2):528-538.

Lan WZ*, Lee SC*, She YF, Jiang YX, Luan S#. (2011) Mechanistic analysis of AKT1 regulation by the CBL-CIPK- PP2CA network. Mol Plant, 4:527-536.

Lan WZ*, Wang W, Wang SM, Li LG, Buchanan BB, Lin HX, Gao JP, Luan S# (2010) A rice high-affinity potassium transporter (HKT) conceals a calcium-permeable cation channel. Proc Natl Acad Sci U S A, 107:7089-7094.

Lee SC*, Lan WZ*, Buchanan BB#, Luan S#. (2009) A protein kinase- phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Proc Natl Acad Sci U S A, 106:21419-21424.

Lee SC*, Lan WZ*, Kim BG*, Li LG*, Cheong YH, Pandey GK, Lu G, Buchanan BB#, Luan S# (2007) A protein phosphorylation/dephosphorylation network regulates a plant potassium channel. Proc Natl Acad Sci U S A, 104:15959-15964.

Others：


Cai G*, Wang Y*, Tu G, Chen P, Luan S#, Lan W#. Type A2 BTB Members Decrease the ABA Response during Seed Germination by Affecting the Stability of SnRK2.3 in Arabidopsis. Int J Mol Sci. 2020 Apr 30;21(9):3153.(第一作者：蔡国华博士生/王愿博士）
全文链接：https://www.mdpi.com/1422-0067/21/9/3153

Shen N*, Jing Y*, Tu G, Fu A#, Lan W#. Danger-Associated Peptide Regulates Root Growth by Promoting Protons Extrusion in an AHA2-Dependent Manner in Arabidopsis. Int J Mol Sci.2020 Oct 27;21(21):E7963. doi: 10.3390/ijms**.(第一作者：沈诺博士生/荆彦平博士）
全文链接：https://www.mdpi.com/1422-0067/21/21/7963
Jing Y*, Shen N*, Zheng X, Fu A, Zhao F, Lan W#, Luan S#. Danger-Associated Peptide Regulates Root Immune Responses and Root Growth by Affecting ROS Formation in Arabidopsis. Int J Mol Sci. 2020 Jun 28;21(13):4590. doi: 10.3390/ijms**. (第一作者：荆彦平博士/沈诺博士生）

Yang Y*, Tang RJ, Mu B, Ferjani A, Shi J, Zhang H, Zhao F, Lan WZ#, Luan S#. Vacuolar Proton Pyrophosphatase Is Required for High Magnesium Tolerance in Arabidopsis.Int J Mol Sci. 2018 Nov 16;19(11). pii: E3617. doi: 10.3390/ijms**. (第一作者：杨阳博士后）

Yu J*, Ge H*, Wang X, Tang R, Wang Y, Zhao F, Lan W#, Luan S#, Yang L#. Overexpression of Pyrabactin Resistance-Like Abscisic Acid Receptors Enhances Drought, Osmotic, and Cold Tolerance in Transgenic Poplars. Front Plant Sci. 2017 Oct 13;8:1752. (第一作者：尉静玲博士生/戈海曼博士生）

Ji R*, Zhou L*, Liu J*, Wang Y, Yang L, Zheng Q#, Zhang C, Zhang B, Ge H, Yang Y, Zhao F, Luan S, Lan W#. Calcium-dependent protein kinase CPK31 interacts with arsenic transporter AtNIP1;1 and regulates arsenite uptake in Arabidopsis thaliana. PLoS One. 2017 Mar 15;12(3):e**.(第一作者：纪瑞杰硕士生）

Yu J*, Li H, Peng Y, Yang L, Zhao F, Luan S#, Lan W#. A survey of the pyrabactin resistance-like abscisic acid receptor gene family in poplar. Plant Signal Behav. 2017 Aug 3;12(8):e**. (第一作者：尉静玲博士生）

Liu J*, Fu S, Yang L, Luan M, Zhao F, Luan S#, Lan W#. Vacuolar SPX-MFS transporters are essential for phosphate adaptation in plants. Plant Signal Behav. 2016;11(8):e**.(第一作者：刘金隆博士生）

Yu J*, Yang L*, Liu X, Tang R, Wang Y, Ge H, Wu M, Zhang J, Zhao F, Luan S#, Lan W#. Overexpression of Poplar Pyrabactin Resistance-Like Abscisic Acid Receptors Promotes Abscisic Acid Sensitivity and Drought Resistance in Transgenic Arabidopsis. PLoS One. 2016;11(12):e**.(第一作者：尉静玲博士生/杨磊副研究员）

Luan S*#, Lan WZ, Lee SC. (2009) Potassium nutrition, sodium toxicity, and calcium signaling: connections through the CBL-CIPK network. Current Opinion in Plant Biology, 12:339-346.

Lan WZ*, Wang PYT, Hill CE. (2006) Modulation of hepatocellular swelling-activated K+ currents by phosphoinositide pathway-dependent protein kinase C. American Journal of Physiology-Cell Physiology, 291:93-103.

Lan WZ*, Abbas H, Lam HD, Lemay AM, Hill CE. (2005) Contribution of a time-dependent and hyperpolarization-activated chloride conductance to currents of resting and hypotonically shocked rat hepatocytes. American Journal of Physiology-Gastrointestinal and Liver Physiology, 288:G221-G229.

Lan WZ*, Abbas H, Lemay AM, Briggs MM, Hill CE. (2005) Electrophysiological and molecular identification of hepatocellular volume-activated K+ channels. Biochimica Et Biophysica Acta-Biomembranes, 1668:223-233.

专著：
Wu W, Wang Y, Lee S, Lan WZ, Luan S. Regulation of Ion Channels by the Calcium Signaling Network in Plant Cells. Demidchik V and Maathuis F (eds.) Ion channels and plant stress responses. Signaling and communication in plants. 2010, Springer-Verlag Berlin Heidelberg.