Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 江培勇教授 (化学病理学系) |
| 卢煜明教授 (化学病理学系) |
| 赵慧君教授 (化学病理学系) |
| 赖宝山教授 (外科学系) |
| 陈力元教授 (内科及药物治疗学系) |
| 莫树锦教授 (肿瘤学系) |
| 陈林教授 (肿瘤学系) |
| 郑淑?博士 (病理解剖及细胞学系) |
| 陈君赐教授 (化学病理学系) |
| 陈渭雯博士 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1073/pnas.1500076112 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/46WOS source URL
其它资讯
摘要The analysis of tumor-derived circulating cell-free DNA opens up new possibilities for performing liquid biopsies for the assessment of solid tumors. Although its clinical potential has been increasingly recognized, many aspects of the biological characteristics of tumor-derived cell-free DNA remain unclear. With respect to the size profile of such plasma DNA molecules, a number of studies reported the finding of increased integrity of tumor-derived plasma DNA, whereas others found evidence to suggest that plasma DNA molecules released by tumors might be shorter. Here, we performed a detailed analysis of the size profiles of plasma DNA in 90 patients with hepatocellular carcinoma, 67 with chronic hepatitis B, 36 with hepatitis B-associated cirrhosis, and 32 healthy controls. We used massively parallel sequencing to achieve plasma DNA size measurement at single-base resolution and in a genome-wide manner. Tumor-derived plasma DNA molecules were further identified with the use of chromosome arm-level z-score analysis (CAZA), which facilitated the studying of their specific size profiles. We showed that populations of aberrantly short and long DNA molecules existed in the plasma of patients with hepatocellular carcinoma. The short ones preferentially carried the tumor-associated copy number aberrations. We further showed that there were elevated amounts of plasma mitochondrial DNA in the plasma of hepatocellular carcinoma patients. Such molecules were much shorter than the nuclear DNA in plasma. These results have improved our understanding of the size profile of tumor-derived circulating cell-free DNA and might further enhance our ability to use plasma DNA as a molecular diagnostic tool.
着者Jiang PY, Chan CWM, Chan KCA, Cheng SH, Wong J, Wong VWS, Wong GLH, Chan SL, Mok TSK, Chan HLY, Lai PBS, Chiu RWK, Lo YMD
期刊名称PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版年份2015
月份3
日期17
卷号112
期次11
出版社NATL ACAD SCIENCES
页次E1317 - E1325
国际标準期刊号0027-8424
语言英式英语
关键词circulating tumor DNA; liquid biopsy; massively parallel sequencing; mitochondrial DNA; tumor markers
Web of Science 学科类别Multidisciplinary Sciences; Science & Technology - Other Topics
