Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 黄振国教授 (化学病理学系) |
| 林伟基教授 (化学病理学系) |
| 许树昌教授 (内科及药物治疗学系) |
| 王紫欣小姐 (化学病理学系) |
| 伦妙芬博士 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1080/08820130903062756 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/20WOS source URL
其它资讯
摘要A recently identified interleukin (IL)-17-producing T-helper (Th) lymphocyte subset, which comprises Th17 cells producing hallmark cytokines IL-17A, IL-17F and IL-22, is involved in chronic inflammatory diseases. Elevated gene and protein expressions of IL-17 are manifested in allergic asthma. We further characterized the activation of Th17 cells in asthmatic patients. Peripheral blood mononuclear cells (PBMC) were purified from 31 asthmatic patients and http://aims.cuhk.edu.hk/converis/portal/Publication/20 sex-and age-matched control subjects. The number of IL-17A secreting cells in peripheral blood was enumerated by enzyme-linked immunosorbent spot assay. Cell surface expression of Th17-related chemokine receptor CCR6, and plasma level of IL-17A, IL-17F and IL-22, and ex vivo production of IL-17A and IL-22 were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. The number of peripheral Th17 lymphocytes, expression of CCR6 on Th cells, and ex vivo IL-23, anti-CD3 and anti-CD28 induced production of IL-22 by PBMC were significantly elevated in asthmatic patients compared with control subjects (all p < 0.01). This clinical study further confirmed increased number of peripheral Th17 lymphocytes and cell surface expression of CCR6 receptors on Th cells in asthmatic patients. Pro-inflammatory cytokine IL-23 can exacerbate disease severity by activating pathogenic Th17 lymphocytes to release downstream inflammatory cytokine IL-22 in asthma.
着者Wong CK, Lun SWM, Ko FWS, Wong PTY, Hu SQ, Chan IHS, Hui DSC, Lam CWK
期刊名称IMMUNOLOGICAL INVESTIGATIONS
出版年份http://aims.cuhk.edu.hk/converis/portal/Publication/2009
月份1
日期1
卷号38
期次7
出版社TAYLOR & FRANCIS INC
页次652 - 664
国际标準期刊号0882-0139
语言英式英语
关键词Asthma; CCR6; IL-22; Th17 cells
Web of Science 学科类别Immunology; IMMUNOLOGY
