Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 锺天恩博士 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1038/nmeth880 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/28WOS source URL
Scopushttp://aims.cuhk.edu.hk/converis/portal/Publication/31Scopus source URL
其它资讯
摘要Human cancers and some congenital traits are characterized by cytogenetic aberrations including translocations, amplifications, duplications or deletions that can involve gain or loss of genetic material. We have developed a simple method to precisely delineate such regions with known or cryptic genomic alterations. Molecular copy-number counting (MCC) uses PCR to interrogate miniscule amounts of genomic DNA and allows progressive delineation of DNA content to within a few hundred base pairs of a genomic alteration. As an example, we have located the junctions of a recurrent nonreciprocal translocation between chromosomes 3 and 5 in human renal cell carcinoma, facilitating cloning of the breakpoint without recourse to genomic libraries. The analysis also revealed additional cryptic chromosomal changes close to the translocation junction. MCC is a fast and flexible method for characterizing a wide range of chromosomal aberrations. ? 2006 Nature Publishing Group.
着者Daser A., Thangavelu M., Pannell R., Forster A., Sparrow L., Chung G., Dear P.H., Rabbitts T.H.
期刊名称Nature Methods
出版年份2006
月份6
日期1
卷号3
期次6
出版社Nature Publishing Group
出版地United Kingdom
页次447 - 453
国际标準期刊号1548-7091
语言英式英语
