Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 卢煜明教授 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1196/annals.1368.023 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/3WOS source URL
其它资讯
摘要The detection of cell-free RNA in plasma and serum of human subjects has found increasing applications in the field of medical diagnostics. However, many questions regarding the biology of circulating RNA remain to be addressed. One issue concerns the molecular nature of these circulating RNA species. We have recently developed a simple and quantitative method to investigate the integrity of plasma RNA. Our results have suggested that cell-free RNA in plasma is generally present as fragmented molecules instead of intact transcripts, with a predominance of 5' fragments. In this article, we summarize the basic principles in the experimental design for plasma RNA integrity analysis and highlight some of the important technical considerations for this type of investigation.
着者Wong BCK, Lo YMD
会议名称4th International Symposium on Circulating Nucleic Acids in Plasma/Serum
会议开始日04.09.2005
会议完结日06.09.2005
会议地点London
期刊名称Annals of the New York Academy of Sciences
出版年份2006
月份1
日期1
卷号1075
出版社BLACKWELL PUBLISHING
页次174 - 178
国际标準书号1-57http://aims.cuhk.edu.hk/converis/portal/Publication/3http://aims.cuhk.edu.hk/converis/portal/Publication/31-627-X
国际标準期刊号0077-892http://aims.cuhk.edu.hk/converis/portal/Publication/3
电子国际标準期刊号1749-66http://aims.cuhk.edu.hk/converis/portal/Publication/32
语言英式英语
关键词integrity; plasma RNA; quantitative analysis
Web of Science 学科类别Biochemistry & Molecular Biology; Medical Laboratory Technology; Multidisciplinary Sciences; Science & Technology - Other Topics
