Refereed conference paper presented and published in conference proceedings
香港中文大学研究人员 ( 现职)
| 崔耀隆教授 (化学病理学系) |
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Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/0WOS source URL
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摘要BRE (Brain and Reproductive Expressed) is a highly conserved, putative stress-modulating gene. It has been shown able to bind the cytoplasmic region of p55 tumor necrosis factor receptor 1 (TNFR-1), whose activation through binding with its ligand, TNF-alpha, triggers both the apoptosis and pro-survival pathways, the latter of which is mediated by activation of NF-kappaB. Overexpression of BRE has also been shown inhibitory to the activation of NF-kappaB by TNF-alpha. This, we surmised, would skew the effect of TNF-alpha towards apoptosis. In this study, we investigated the functional consequence of BRE overexpression on TNF-induced apoptosis, and sought to confirm the above prediction. Our data, however, demonstrated the opposite result. Using both the human and mouse cell lines, HeLa and D122, respectively, we found that overexpression of BRE in these cells through stable transfection with the BRE expression construct, pcDNA3-BRE, resulted in resistance to TNF-alpha-induced apoptosis, in the presence of cycloheximide. Thus, we have shown that despite the reported ability of BRE in down-regulating the survival-promoting NF-kappaB activity induced by TNF-alpha, BRE can inhibit the apoptosis induced by the same cytokine.
着者Li Q, Ching AKK, Chan JYH, Miao J, Lim PL, Chui YL
会议名称15th IFCC/FESCC European Congress of Clinical Chemistry and Laboratory Medicine
会议开始日http://aims.cuhk.edu.hk/converis/portal/Publication/01.http://aims.cuhk.edu.hk/converis/portal/Publication/06.2http://aims.cuhk.edu.hk/converis/portal/Publication/0http://aims.cuhk.edu.hk/converis/portal/Publication/03
会议完结日http://aims.cuhk.edu.hk/converis/portal/Publication/05.http://aims.cuhk.edu.hk/converis/portal/Publication/06.2http://aims.cuhk.edu.hk/converis/portal/Publication/0http://aims.cuhk.edu.hk/converis/portal/Publication/03
会议地点BARCELONA
会议国家西班牙
出版年份2http://aims.cuhk.edu.hk/converis/portal/Publication/0http://aims.cuhk.edu.hk/converis/portal/Publication/03
月份1
日期1
出版社MEDIMOND S R L
页次629 - 632
国际标準书号88-323-3128-4
语言英式英语
Web of Science 学科类别Biochemistry & Molecular Biology; Medical Laboratory Technology
