Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 陈锦荣先生 (化学病理学系) |
| 潘力行教授 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/S0039-128X(02)00183-6 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/19WOS source URL
其它资讯
摘要The mouse Leydig tumor cells (MLTC-1) were derived from a transplantable Leydig cell tumor carried in C57BL/6 mice. The original cell line (M5480) produced testosterone and little progesterone. However, it was later shown that there were two subtypes of the cell line, one producing mainly progesterone and termed M5480P and the other which produced androgens and termed M5480A. MLTC-1 cells are reportedly derived from the former. We studied the production of testosterone by MLTC-1 cells using a specific and sensitive testosterone RlA, tandem mass spectrometry (TMS) and examined the expression of mRNA of some key enzymes involved in steroidogenesis. Although the molar yields were 1:20:60 for testosterone, androstenedione and progesterone, respectively, in response to human chorionic gonadotropin (hCG), testosterone measured by our RIA accounted for 94% of the testosterone immunoreactivity. Both MLTC-1 and Balb/c Leydig cells expressed Steroidogenic Acute Response (StAR) protein mRNA in response to hCG. Cytochrome P450 17alpha-hydroxylase/17,20-lyase mRNA was expressed constitutively in MLTC-1 and Balb/c Leydig cells. Whereas the latter expressed 17beta-hydroxydehydrogenase/17-ketoreductase isoform Type 3 mRNA in response to hCG, MLTC-1 cells expressed isoform Type 7 constitutively. The absence of isoform Type 3 in MLTC-1 cells thus may account for the low conversion of androstenedione to testosterone in this cell line. However, with a very specific and sensitive RIA even the low production of testosterone becomes meaningful. In conclusion MLTC-1 cells produce testosterone. (C) 2002 Elsevier Science Inc. All rights reserved.
着者Panesar NS, Chan KW, Ho CS
期刊名称STEROIDS
出版年份2003
月份3
日期1
卷号68
期次3
出版社ELSEVIER SCIENCE INC
页次245 - 251
国际标準期刊号0039-128X
电子国际标準期刊号1878-5867
语言英式英语
关键词17 beta-hydroxydehydrogenase/17-ketoreductase Leydig cells; cytochrome P450 17 alpha-hydroxylase/17,20-lyase; RT-PCR; Steroidogenic Acute Response protein; tandem mass spectroscopy
Web of Science 学科类别Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Endocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM
