Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 谭兆祥教授 (生物医学学院) |
| 黎永祥教授 (病理解剖及细胞学系) |
| 邓亮生教授 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1177/019262330002800501 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/16WOS source URL
其它资讯
摘要This study focused on the subacute renal lesions resulting from the infusion of stroma free hemoglobin (SFH), which remains under evaluation as a potential blood substitute despite limited renal toxicity observed in acute infusion. Four groups of rats received different doses of SFH (0.03, 0.48, 0.96, and 1.46 g, respectively) and were monitored, on alternate days, for their glomerular filtration rate over the course of 10 days. Another group of 6 rats receiving 0.96 g SFH was sacrificed at day 10 for examination of renal morphology. The low dose (0.03 g) of SFH infusion did not alter the creatinine clearance (Clcr) over 10 days. The Clcr decreased in rats receiving 0.48 g SFH but fully recovered at day 10. A persistent decrease in Clcr was observed in the groups of rats receiving 0.96 and 1.68 g of SFH. Tubular necrosis was the most prominent renal lesion distributed in the proximal tubules, especially in the convoluted segment of the juxtamedullary nephrons. Pearls' stained cytoplasmic granules and electron-dense lysosomal granules were found in surviving proximal tubules. Necrosis was the predominant mechanism of cell death. This study revealed for the first time proliferation of smooth endoplasmic reticulum in the proximal tubules after SFH treatment, where it appeared as nodular aggregates of tubulovesicular structures. The effect of SFH on the proximal tubule appeared to be a direct toxicity, and this toxicity was shown to be dose dependent. The presence of reversible toxicity indicated that a safety limit dosage for SFH infusion exists and that tolerance dose of SFH can be determined for clinical applications.
着者Chan WL, Tang NLS, Yim CCW, Lai FM, Tam MSC
期刊名称Toxicologic Pathology
出版年份2000
月份9
日期1
卷号28
期次5
出版社SOC TOXICOLOGIC PATHOLOGISTS
页次635 - 642
国际标準期刊号0192-6233
语言英式英语
关键词acute tubular necrosis; blood substitutes; glomerular filtration rate; proximal renal tubule; smooth endoplasmic reticulum
Web of Science 学科类别Pathology; PATHOLOGY; Toxicology; TOXICOLOGY
