骆观正

Submitted on 周一, 10/30/2017 - 20:32


性别
男

个人主页
http://epigenome.sysu.edu.cn/

电子邮件
luogzh5@mail.sysu.edu.cn

职称
教授

导师类型
博士生导师

学历
博士

学科方向
0710生物学-10生物化学与分子生物学
0710生物学-Z1生物信息学


研究方向
1. 高通量技术与表观遗传组
2. 生物信息与基因表达调控
3. 合成生物学与生命起源
生命科学经过前期的发展，理论框架已基本完善。研究方法的不断创新和数据的积累让研究者摆脱了“集邮式”的研究范式，如今生命科学已经具备了理解世界乃至改变世界的能力。而与化学、物理、信息学等成熟定量学科的深度交叉融合为生命科学在21世纪的大爆发做好了准备。我们正在见证，生命科学在健康、农业、生态、能源等方方面面改变了我们的生活，成为决定国计民生的支柱性学科。
我们实验室将致力于继续发展新的高通量工具和分析方法，以表观遗传作为切入点，为最终全面理解遗传物质 - 我们的基因组，做出应有的贡献。另一方面，我们也将把解码的成果尝试应用到人工合成上，探索研究成果的实际应用潜力，并尝试理解生命从无到有，从简单到复杂背后的驱动力量。
招聘信息：实验室研究兴趣广泛，鼓励创新，欢迎对探索生命本质有着强烈兴趣的同学加入。对新入学研究生无背景要求，鼓励培养多学科交叉能力。同时招聘博士后等青年科研人员，希望具有分子生物学/合成生物学或生物信息学/系统生物学背景，有开辟新方向的志向和能力。因课题需要，特别欢迎具备大规模基因组操控或定向进化经验的有志青年加入。
欢迎扫描以下二维码，关注公众号，了解实验室最新动态。（如不能正常显示图片可搜索公众号关注：luolab2017 ）
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个人经历
2020-至今，有害生物控制与资源利用 国家重点实验室，PI
2017-至今，基因功能与调控 教育部重点实验室，PI
2017-至今，中山大学生命科学学院，教授
2013-2017，芝加哥大学，生物分子动态研究所，博士后（合作导师：何川）
2010-2013，中科院遗传发育所，分子系统生物学中心，助理研究员
2005-2010，中科院遗传发育所，生物信息学，理学博士 （导师：王秀杰）
2001-2005，东南大学，生物医学工程，工程学士
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讲授课程
分子生物学（主讲，2019-）
现代生命科学进展（参与，2019-）
专业科技文献阅读和理解（参与，2018-）
基因组时代的进化与计算生物学（参与，2018）
医疗新时代：精准个性化医疗（主讲，2017-2018）

学术成就
随着人类基因组计划的完成和测序技术的发展，我们获得生物数据的能力得到革命性的提升，而如何解析和利用这些数据，成为了当今生命科学领域的焦点。基因之间的互作及调控规律是最基础的问题之一，研究基因调控对我们理解生长发育、遗传变异、生理生态，乃至人类疾病，都有着极为重要的意义。基因调控大致可以分为转录前和转录后两个层面。转录前调控的决定因素之一是DNA修饰。之前的研究基本集中在5位甲基胞嘧啶上（5mC），对其它修饰类型的研究还非常稀缺。转录后调控则更加复杂，其中关于非编码RNA介导的基因沉默已得到深入的研究，而编码基因本身是否受到修饰的调控是目前研究的焦点。课题组在基因的调控规律方面有良好的积累，特别是围绕着新型DNA和RNA修饰介导的基因调控模式开展了持续性的研究。在转录前调控方面，开发出针对DNA N6甲基腺嘌呤（6mA）修饰的高通量测序方法，并首次揭示了真核生物中6mA的全基因组分布图谱，发现6mA和染色质的互作规律及对基因转录的调控功能。在转录后调控方面，发现以m6A为代表的RNA修饰是调节基因表达的重要途径之一，并通过发展单碱基分辨率检测技术m6A-REF-seq，首次发现m6A的特殊分布模式对基因表达的正调控作用，而有别于之前报道的mRNA降解模型，对m6A机制的解析起到很好的补充。课题组成员为主要贡献作者的论文发表在Cell、Science Advances、Cell Research、Genome Biology、Nature Communications、Bioinformatics等杂志上。

承担课题
主持
国自然优秀青年科学基金项目（2020-2022）
国自然面上项目（2019-2022）
国自然重大研究计划培育项目（2018-2020）
国自然应急管理项目 （2018-2018）
广东省“青年珠江****”（2018-2021）
广东省自然科学****基金（2018-2022）
中山大学青年教师重点培育项目（2018-2019）
中山大学“****”启动项目（2017-2020）
参与
国家重点研发计划“发育编程及其代谢调节”重点专项（2020-2024）
中山大学重大项目培育和新兴学科交叉学科资助计划项目（2019-2020）
国家重点研发计划“干细胞及转化研究”重点专项（2018-2023）
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论文专著
（本实验室人员姓名加粗，第一作者标#，通讯作者标*）
实验室成员作为主要贡献作者发表论文：
Chen X#,?Zhao?Q#, Zhao YL#,?Chai GS, Cheng?W, Zhao Z, Wang?J*, Luo GZ*, Cao Nan*.?Targeted RNA N6‐Methyladenosine Demethylation Controls Cell Fate Transition in Human Pluripotent Stem Cells. Advanced Science. (in press)
Wang M#, Xiao Y#, Li Y#, Wang X, Qi S, Wang Y, Zhao L, Wang K, Peng W, Luo GZ*, Xue X*, Jia G*, Wu L*. RNA m6A? Modification? Functions in Larval Development and Caste Differentiation in Honeybee (Apis mellifera). Cell Reports.?34(1):108580 (2021)
Chen LQ#, Zhao WS, Luo GZ*.?Mapping and editing of nucleic acid modifications.?Computational and? Structural? Biotechnology?Journal. 18:661-667 (2020)
Zhang?Z#, Chen LQ,?Zhao YL,?Yang CG,? Roundtree I,?Zhang Z,?Ren J,?Xie W,?He C,?Luo GZ*.?Single-base mapping of m6A by an antibody-independent method. Science Advances. 5(7):eaax0250 (2019)
Lin X#, Chai G#, Wu Y#, Li J, Chen F, Liu J, Luo G, Tauler J, Du J, Lin S, He C, Wang H*.?RNA m6A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.?Nature Communications.?10: 2065 (2019)?
Liu J#, Luo G#, Sun J, Men L, Ye H, He C, Ren D*.?METTL14 is essential for β-cell survival and insulin secretion.?Biochim Biophys Acta Mol Basis Dis. 1865(9):2138-2148 (2019)
Wang X#, Li Y#, Chen W, Shi H, Eren AM, Morozov A, He C, Luo GZ*, Pan T*.?Transcriptome-wide reprogramming of N6-methyladenosine modification by the mouse microbiome.?Cell Research. 29(2):167-170 (2019)
Luo GZ*#,?Hao Z#,?Luo L,?Shen M,?Sparvoli D,?Zheng Y,?Zhang Z,?Weng X,?Chen K,?Cui Q, Turkewitz AP and?He C*.?N6-methyldeoxyadenosine directs nucleosome positioning in?Tetrahymena?DNA. Genome Biology.?19:200 (2018)
Luo GZ & He C. DNA N6-methyladenine in metazoans: functional epigenetic mark or bystander? Nature Structural & Molecular Biology. 24(6):503-506 (2017)
Liu J#, Zhu Y#, Luo GZ#, Wang X#, Yue Y, Wang X, Zong X, Chen K, Yin H, Fu Y, Han D, Wang Y, Chen D, He C. Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig. Nature Communications. 7:13052 (2016)
Luo GZ#, Wang F#, Weng X, Chen K, Hao Z, Yu M, Deng X, Liu J, He C. Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing. Nature Communications. 7:11301 (2016)
Luo GZ, Blanco MA, Greer EL, He C, Shi Y. DNA N6-methyladenine: a new epigenetic mark in eukaryotes? Nature Reviews Molecular Cell Biology. 16(12):705-10 (2015)
Fu Y#, Luo GZ#, Chen K, Deng X, Yu M, Han D, Hao Z, Liu J, Lu X, Doré LC, Weng X, Ji Q, Mets L, He C. N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas. Cell. 161(4):879-92 (2015)?
Luo GZ#, MacQueen A#, Zheng G#, Duan H, Dore LC, Lu Z, Liu J, Chen K, Jia G*, Bergelson J*, He C*. Unique features of the m6A methylome in Arabidopsis thaliana. Nature Communications. 5:5630 (2014)
Luo GZ, Yang W, Ma YK, Wang XJ. ISRNA: an integrative online toolkit for short reads from high-throughput sequencing data. Bioinformatics. 30(3):434-6 (2014)
Luo GZ#, Hafner M#, Shi Z, Brown M, Feng GH, Tuschl T, Wang XJ*, Li X*. Genome-wide annotation and analysis of zebra finch microRNA repertoire reveal sex-biased expression. BMC Genomics. 13:727 (2012)
Liu L#, Luo GZ#, Yang W#, Zhao X#, Zheng Q, Lv Z, Li W, Wu HJ, Wang L, Wang XJ, Zhou Q. Activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells. Journal of Biological Chemistry. 285(25):19483-90 (2010)?
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实验室成员作为合作作者参与发表论文：
Liu J, Gao M, He J, Wu K, Lin S, Jin L, Chen Y, Liu H, Shi J, Wang X, Chang L, Lin Y, Zhao YL, Zhang X, Zhang M, Luo GZ, Wu G, Pei D, Wang J, Bao X, Chen J.?The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity. Nature.?591(7849):322-326 (2021)
Liu X, Chen R, Sun Y, Chen R, Zhou J, Tian Q, Tao X, Zhang Z, Luo GZ, Xie W.?Crystal structure of the yeast heterodimeric ADAT2/3 deaminase. BMC Biology.?18(1):189 (2020)
Xie D, Tong M, Xia B, Feng G, Wang L, Li A, Luo G, Wan H, Zhang Z, Zhang H, Yang YG, Zhou Q, Wang M, Wang XJ.?Long noncoding RNA lnc-NAP sponges mmu-miR-139-5p to modulate Nanog functions in mouse ESCs and embryos.?RNA Biology. 23:1-13 (2020)
Du G, Xiong L, Li X, Zhuo Z, Zhuang X, Yu Z, Wu L, Xiao D, Liu Z, Jie M, Liu X, Luo G, Guo Z, Chen H.?Peroxisome Elevation Induces Stem Cell Differentiation and Intestinal Epithelial Repair.?Developmental?Cell. (20)30186-6 (2020)
Weng X, Gong J, Chen Y, Wu T, Wang F, Yang S, Yuan Y, Luo G, Chen K, Hu L, Ma H, Wang P, Zhang QC, Zhou X, He C. Keth-seq for transcriptome-wide RNA structure mapping.?Nature?Chemical Biology. doi: 10.1038/s41589-019-0459-3 (2020)
Zhang LS#, Liu C, Ma H, Dai Q, Sun HL, Luo G, Zhang Z, Zhang L, Hu L, Dong X, He C*.?Transcriptome-wide Mapping of Internal N7-Methylguanosine Methylome in Mammalian mRNA.?Molecular?Cell. 74(6):1304-1316 (2019)
Chen Z#, Qi M, Shen B, Luo G, Wu Y, Li J, Lu Z, Zheng Z, Dai Q, Wang H*.?Transfer RNA demethylase ALKBH3 promotes cancer progression via induction of tRNA-derived small RNAs.?Nucleic Acids Research. 47(5):2533-2545 (2019)
Yue Y, Liu J, Cui X, Cao J, Luo G, Zhang Z, Cheng T, Gao M, Shu X, Ma H, Wang F, Wang X, Shen B, Wang Y, Feng X, He C, Liu J. VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation.?Cell Discovery.? 4:10 (2018)
Zhu S, Beaulaurier J, Deikus G, Wu TP, Strahl M, Hao Z, Luo G, Gregory JA, Chess A, He C, Xiao A, Sebra R, Schadt EE, Fang G. Mapping and characterizing N6-methyladenine in eukaryotic genomes using single-molecule real-time sequencing. Genome Research. 28(7):1067-1078 (2018)
Roundtree IA, Luo GZ, Zhang Z, Wang X, Zhou T, Cui Y, Sha J, Huang X, Guerrero L, Xie P, He E, Shen B, He C. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife. pii: e31311 (2017)
Hsu PJ, Zhu Y, Ma H, Guo Y, Shi X, Liu Y, Qi M, Lu Z, Shi H, Wang J, Cheng Y, Luo G, Dai Q, Liu M, Guo X, Sha J, Shen B, He C. Ythdc2 is an N6-methyladenosine binding protein that regulates mammalian spermatogenesis. Cell Research. 27(9):1115-1127 (2017)
Liu F, Clark W, Luo G, Wang X, Fu Y, Wei J, Wang X, Hao Z, Dai Q, Zheng G, Ma H, Han D, Evans M, Klungland A, Pan T, He C. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell. 167(3): 816-828 (2016)
Feng G, Tong M, Xia B, Luo GZ, Wang M, Xie D, Wan H, Zhang Y, Zhou Q, Wang XJ. Ubiquitously expressed genes participate in cell-specific functions via alternative promoter usage. EMBO Reports. 17(9):1304-13 (2016)
Chen K, Luo GZ, He C. High-Resolution Mapping of N?-Methyladenosine in Transcriptome and Genome Using a Photo-Crosslinking-Assisted Strategy. Methods Enzymol. 560:161-85 (2015)
Shuai L, Wang Y, Dong M, Wang X, Sang L, Wang M, Wan H, Luo G, Gu T, Yuan Y, Feng C, Teng F, Li W, Liu X, Li T, Wang L, Wang XJ, Zhao XY, Zhou Q. Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro. J Mol Cell Biol. 7(4):326-37 (2015)
Deng X, Chen K, Luo GZ, Weng X, Ji Q, Zhou T, He C. Widespread occurrence of N6-methyladenosine in bacterial mRNA. Nucleic Acids Research. 43(13):6557-67 (2015)
Chen K, Lu Z, Wang X, Fu Y, Luo GZ, Liu N, Han D, Dominissini D, Dai Q, Pan T and He C. High-Resolution N6-Methyladenosine (m6A) Map Using Photo-Crosslinking-Assisted m6A Sequencing. Angew Chem Int Ed Engl. 54(5):1587-90 (2015)
Li W, Li X, Li T, Jiang MG, Wan H, Luo GZ, Feng C, Cui X, Teng F, Yuan Y, Zhou Q, Gu Q, Shuai L, Sha J, Xiao Y, Wang L, Liu Z, Wang XJ, Zhao XY, Zhou Q. Genetic modification and screening in rat using haploid embryonic stem cells. Cell Stem Cell. 14(3):404-14 (2014)
Fu L, Shi Z, Luo G, Tu W, Wang X, Fang Z, Li X. Multiple microRNAs regulate human FOXP2 gene expression by targeting sequences in its 3' untranslated region. Mol Brain. 7:71 (2014)
Shi Z, Luo G, Fu L, Fang Z, Wang X, Li X. miR-9 and miR-140-5p target FoxP2 and are regulated as a function of the social context of singing behavior in zebra finches. J Neurosci. 33(42):16510-21 (2013)
Wan H, He Z, Dong M, Gu T, Luo GZ, Teng F, Xia B, Li W, Feng C, Li X, Li T, Shuai L, Fu R, Wang L, Wang XJ, Zhao XY, Zhou Q. Parthenogenetic haploid embryonic stem cells produce fertile mice. Cell Research. 23(11):1330-3 (2013)
Zhang Y, Teng F, Luo GZ, Wang M, Tong M, Zhao X, Wang L, Wang XJ, Zhou Q. MicroRNA-323-3p regulates the activity of polycomb repressive complex 2 (PRC2) via targeting the mRNA of embryonic ectoderm development (Eed) gene in mouse embryonic stem cells. J Biol Chem. 288(33):23659-65 (2013)
Li RC, Tao J, Guo YB, Wu HD, Liu RF, Bai Y, Lv ZZ, Luo GZ, Li LL, Wang M, Yang HQ, Gao W, Han QD, Zhang YY, Wang XJ, Xu M, Wang SQ. In Vivo Suppression of MiR-24 Prevents the Transition toward Decompensated Hypertrophy in Aortic-constricted Mice. Circ Res. 112(4):601-5 (2013)
Han J, Chen L, Luo G, Dai B, Wang X, Dai J. Three-dimensional culture may promote cell reprogramming. Organogenesis. 9(2):118-20 (2013)
Liu X, Zhang Y, Tong M, Liu XY, Luo GZ, Xie DF, Ren SF, Bai DH, Wang L, Zhou Q, Wang XJ. Identification of a small molecule 1,4-bis-[4-(3-phenoxy-propoxy)-but-2-ynyl]-piperazine as a novel inhibitor of the transcription factor p53. Acta Pharmacol Sin. 34(6):805-10 (2013)
Li W, Shuai L, Wan H, Dong M, Wang M, Sang L, Feng C, Luo GZ, Li T, Li X, Wang L, Zheng QY, Sheng C, Wu HJ, Liu Z, Liu L, Wang L, Wang XJ, Zhao XY, Zhou Q. Androgenetic haploid embryonic stem cells produce live transgenic mice. Nature. 490(7420):407-11 (2012)
Xu M, Wu HD, Li RC, Zhang HB, Wang M, Tao J, Feng XH, Guo YB, Li SF, Lai ST, Zhou P, Li LL, Yang HQ, Luo GZ, Bai Y, Xi JJ, Gao W, Han QD, Zhang YY, Wang XJ, Meng X, Wang SQ. Mir-24 Regulates Junctophilin-2 Expression in Cardiomyocytes. Circ Res. 111(7):837-41 (2012)
Zhang C, Xu Y, Guo S, Zhu J, Huan Q, Liu H, Wang L, Luo G, Wang X, Chong K. Dynamics of brassinosteroid response modulated by negative regulator LIC in rice. PLoS Genet. 8(4):e** (2012)
Du Y, Gao C, Liu Z, Wang L, Liu B, He F, Zhang T, Wang Y, Wang X, Xu M, Luo GZ, Zhu Y, Xu Q, Wang X, Kong W. Upregulation of a disintegrin and metalloproteinase with thrombospondin motifs-7 by miR-29 repression mediates vascular smooth muscle calcification. Arterioscler Thromb Vasc Biol. 32(11):2580-8 (2012)
Zhou Y, Li S, Qian Q, Zeng D, Zhang M, Guo L, Liu X, Zhang B, Deng L, Liu X, Luo G, Wang X, Li J. BC10, a DUF266-containing and Golgi-located type II membrane protein, is required for cell-wall biosynthesis in rice (Oryza sativa L.). Plant J. 57(3):446-62 (2009)
Liu X, Luo G, Bai X, Wang XJ. Bioinformatic analysis of microRNA biogenesis and function related proteins in eleven animal genomes. J Genet Genomics. 36(10):591-601 (2009)