王晓君1,
吴俊斌1,
郭焱1,2,
张召基1,
陈少华1
1.中国科学院城市环境研究所，城市污染物转化重点实验室，厦门 361021
2.中国科学院大学，北京 100049
基金项目: 福建省自然科学基金资助项目（2015J05115）
中国科学院城市环境研究所青年前沿项目（IUEMS201404）
Rapid start-up strategy and microbial characteristics of anammox process
YANG Ruili1,2,WANG Xiaojun1,
WU Junbin1,
GUO Yan1,2,
ZHANG Zhaoji1,
CHEN Shaohua1
1.Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
-->
摘要
HTML全文
图
参考文献
相关文章
施引文献
资源附件
访问统计
摘要:为探讨种泥投加及氮负荷提升方式对厌氧氨氧化（anaerobic ammonia oxidation, anammox）工艺启动中微生物丰度及群落结构的影响，采取先普通活性污泥驯化后再接种anammox种泥的方式启动anammox工艺。结果表明在活性迟滞阶段投加anammox菌种可以快速启动anammox工艺。通过缩短水力停留时间的方式增加氮负荷并可以避免直接提高进水氮浓度导致的基质毒性抑制，有利于达到更高的总氮去除负荷。稳定运行时反应器的氮去除负荷达0.51 kg·(m3·d)-1，anammox菌基因丰度为4.92×109 copies·g-1 (以VSS计)，占细菌总数的2.70%。启动阶段，反应器内微生物多样性逐渐下降，检测到浮霉菌门中4个anammox菌属，以Candidatus Jettenia和Candidatus Kuenenia为主要anammox菌属。在接种污泥处于活性迟滞阶段时，结合提高进水氮浓度、缩短水力停留时间和投加anammox菌种的方式可以快速启动anammox工艺。
关键词: 厌氧氨氧化（anammox）/
生物脱氮/
微生物特性/
快速启动
Abstract:In order to investigate the effects of seed sludge inoculation and increase mode for the influent nitrogen load on the microbial abundance and community in anaerobic ammonia oxidation (anammox) reactor during a start-up period, the anammox process was initiated by inoculating acclimated activated sludge with anammox seed sludge. Results showed that adding anammox strain at a lag stage was beneficial to the rapid start-up of pilot-scale reactor. An improved nitrogen removal rate (NRR) was achieved through shortening hydraulic retention time as well as increasing the influent nitrogen load, which was an effective means to avoid the inhibition of matrix toxicity caused by increasing the influent TN concentration. In the steady running phase, the NRR of 0.51 kg·(m3·d)-1 was realized, and the gene abundance of anammox bacteria reached 4.92×109 copies·g-1 (calculated by VSS), accounting for 2.70% of the total bacteria. In the start-up phase, the microbial diversity in the reactor gradually decreased, four anammox bacteria genus belonging to Planctomycetes were identified, and the dominant genus of functional bacteria were Candidatus Jettenia and Candidatus Kuenenia. The anammox process was successfully start-up with short term by inoculating anammox seed sludge, raising influent nitrogen and shorting hydraulic retention time at the lag stage of inoculation sludge.
Key words:anaerobic ammonia oxidation (anammox)/
biological removal of nitrogen/
microorganism characteristics/
rapid start-up.
| [1] | WR V D S, ABMA W R, BLOMMERS D, et al.Startup of reactors for anoxic ammonium oxidation: experiences from the first full-scale anammox reactor in Rotterdam[J].Water Research,2007,41(18):4149-4163 10.1016/j.watres.2007.03.044 |
| [2] | TANG C, PING Z, YI Y U, et al.Monosodium glutamate wastewater treatment with a full-scale SHARON reactor[J].Acta Scientiae Circumstantiae,2008,28(11):2228-2235 |
| [3] | DENG L W, ZHENG P, CHEN Z A.Anaerobic digestion and post-treatment of swine wastewater using IC-SBR process with bypass of raw wastewater[J].Process Biochemistry,2006,41(4):965-969 10.1016/j.procbio.2005.10.022 |
| [4] | TANG C J, ZHENG P, CHEN T T, et al.Enhanced nitrogen removal from pharmaceutical wastewater using SBA-ANAMMOX process[J].Water Research,2011,45(1):201-210 10.1016/j.watres.2010.08.036 |
| [5] | LU Y F, MA L J, MA L, et al.Improvement of start-up and nitrogen removal of the anammox process in reactors inoculated with conventional activated sludge using biofilm carrier materials[J].Environmental Technology,2017,39(1):59-67 10.1080/09593330.2017.1294624 |
| [6] | IBRAHIM M, YUSOF N, MOHD Z M Y, et al.Enrichment of anaerobic ammonium oxidation (anammox) bacteria for short start-up of the anammox process: A review[J].Desalination & Water Treatment,2016,57(30):13958-13978 10.1080/19443994.2015.1063009 |
| [7] | CHAMCHOI N, NITISORAVUT S.Anammox enrichment from different conventional sludges[J].Chemosphere,2007,66(11):2225-2232 10.1016/j.chemosphere.2006.09.036 |
| [8] | TAO Y, GAO D W, FU Y, et al.Impact of reactor configuration on anammox process start-up: MBR versus SBR[J].Bioresource Technology,2012,104(1):73-80 10.1016/j.biortech.2011.10.052 |
| [9] | YIN X, QIAO S, ZHOU J, et al.Fast start-up of the anammox process with addition of reduced graphene oxides[J].Chemical Engineering Journal,2016,283:160-166 10.1016/j.cej.2015.07.059 |
| [10] | 唐崇俭, 郑平, 陈建伟,等. 中试厌氧氨氧化反应器的启动与调控[J]. 生物工程学报,2009,25(3):406-412 |
| [11] | WANG G, XU X, ZHOU L, et al.A pilot-scale study on the start-up of partial nitrification-anammox process for anaerobic sludge digester liquor treatment[J].Bioresource Technology,2017,241:181-189 10.1016/j.biortech.2017.02.125 |
| [12] | YE L, LI D, ZHANG J, et al.Fast start-up of anammox process with mixed activated sludge and settling option[J].Environmental Technology,2017,19:1-8 10.1080/09593330.2017.1375016 |
| [13] | WANG T, ZHANG H, YANG F, et al.Start-up and long-term operation of the anammox process in a fixed bed reactor (FBR) filled with novel non-woven ring carriers[J].Chemosphere, 2013,91(5):669-675 10.1016/j.chemosphere.2013.01.026 |
| [14] | WETT B.Solved upscaling problems for implementing deammonification of rejection water[J].Water Science & Technology,2006,53(12):121-128 10.2166/wst.2006.413 |
| [15] | WANG Y, MA X, ZHOU S, et al.Expression of the nirS, hzsA, and hdh genes in response to nitrite shock and recovery in candidatus kuenenia stuttgartiensis[J].Environmental Science & Technology,2016,50(13):6940-6947 10.1021/acs.est.6b00546 |
| [16] | CAO S, DU R, LI B, et al.High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater[J].Applied Microbiology & Biotechnology,2016,100(14):6457-6467 10.1007/s00253-016-7427-6 |
| [17] | 曹雁, 王桐屿, 秦玉洁,等. 厌氧氨氧化反应器脱氮性能及细菌群落多样性分析[J]. 环境科学,2017,38(4):1544-1550 10.13227/j.hjkx.201610081 |
| [18] | HENDRICKX T L, KAMPMAN C, ZEEMAN G, et al.High specific activity for anammox bacteria enriched from activated sludge at 10°C[J].Bioresource Technology,2014,163:214-221 10.1016/j.biortech.2014.04.025 |
| [19] | 国家环境保护总局. 水和废水监测分析方法[M].4版. 北京: 中国环境科学出版社,2002:211-213 |
| [20] | TANG C J, ZHENG P, CHAI L Y, et al.Characterization and quantification of anammox start-up in UASB reactors seeded with conventional activated sludge[J].International Biodeterioration & Biodegradation,2013,82(8):141-148 10.1016/j.ibiod.2013.02.014 |
| [21] | HOU L, ZHENG Y, LIU M, et al.Anaerobic ammonium oxidation (anammox) bacterial diversity, abundance, and activity in marsh sediments of the Yangtze Estuary[J].Journal of Geophysical Research Biogeosciences,2014,118(3):1237-1246 10.1002/jgrg.20108 |
| [22] | LI M, HONG Y, KLOTZ M G, et al.A comparison of primer sets for detecting 16S rRNA and hydrazine oxidoreductase genes of anaerobic ammonium-oxidizing bacteria in marine sediments[J].Applied Microbiology & Biotechnology,2010,86(2):781-790 10.1007/s00253-009-2361-5 |
| [23] | BAGCHI S, BISWAS R, ROYCHOUDHURY K, et al.Stable partial nitrification in an up-flow fixed-bed bioreactor under an oxygen-limiting environment[J].Environmental Engineering Science,2009,26(8):1309-1318 10.1089/ees.2008.0389 |
| [24] | GUO J, WANG S, LIAN J, et al.Rapid start-up of the anammox process: Effects of five different sludge extracellular polymeric substances on the activity of anammox bacteria[J].Bioresource Technology,2016,220:641-646 10.1016/j.biortech.2016.08.084 |
| [25] | BI Z, QIAO S, ZHOU J, et al.Fast start-up of anammox process with appropriate ferrous iron concentration[J].Bioresource Technology,2014,170(5):506-512 10.1016/j.biortech.2014.07.106 |
| [26] | JIN R C, ZHENG P, HU A H, et al.Performance comparison of two anammox reactors: SBR and UBF[J].Chemical Engineering Journal,2008,138(1):224-230 10.1016/j.cej.2007.06.038 |
| [27] | STROUS M, HEIJNEN J J, KUENEN J G, et al.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J].Applied Microbiology & Biotechnology,1998,50(5):589-596 10.1007/s002530051340 |
| [28] | WANG S, GUO J, LIAN J, et al.Rapid start-up of the anammox process by denitrifying granular sludge and the mechanism of the anammox electron transport chain[J].Biochemical Engineering Journal,2016,115:101-107 10.1016/j.bej.2016.09.001 |
| [29] | CHEN H, HU H Y, CHEN Q Q, et al.Successful start-up of the anammox process: Influence of the seeding strategy on performance and granule properties[J].Bioresource Technology,2016,211:594-602 10.1016/j.biortech.2016.03.139 |
| [30] | 陈光辉, 李军, 邓海亮,等. 包埋菌启动厌氧氨氧化反应器及其动力学性能[J]. 化工学报,?‰???????匩伺丱?刵???吴漶眶愠爱搰?愱渰‰攷振潳氱漰朵椲挹愭氰?挳氭愱猳猹椷昭楸挼慢瑲椾漠湛″漱晝?獐潕楙汏?戠慄挬琠敃牁楒慖孁?嵁??捒潒汏潙杏礠?㈠き????????????????????ぴ????ぎ?ぴ???????扯牴??孲??崠??佴乲乯?乳?剡??????剳吠?偨????????????敩瑴?慲氠??愠瑴捨桥?敡湮牡業捭桯浸攠湰瑲?潣晥?慳渠慡浴洠潣硯?扭慯据琠数版椠慣?慮湤摩?獩瑯畮摳礮?潊晝?瑂桩敯?略湣摨敮牯汬祯楧湹朠?浥楴捴牥潲扳椬愲氰?挴漬洳洶用渳椩琺礵?搷礭渵愵洱椼换獲嬾?嵛??桝攠浉楓捁態汁??測朠楓湕敍敉牎楏渠杔??潔畓牕湎慅汄?㈠こ????????????????づ??ぶ???橰?捲敦橯??ち??????ㄠ???扥牲??孥??崠???丠???????呴??奥唠創??卬??佩婮????乡??敯瑢?慣氠??捭潯灮桩祵獭椠潯汸潩杤楡捴慩汯?爠潲汥敡?慴湩摯?晳畛湊捝琮楊潯湵?潮晡?甠湯捦甠求瑩畯牳散摩??档汥漠爦漠時汩敯硥楮?楩湮?慥湲?慮湧愬洲洰漰砷?爱攰愳挨琵漩爺嬴?崶?圴愹琰攠爱‰匮挱椲收渳振敪???吱攰挳栮渴漸氶漼杢祲?㈠せ?社????????木????㈠??ㄠ??き??????睆猠瑌??ぶ???????扮爠??嬠??嵴????????佤?????卢??湩慯浮洠潥硦?扥慣獴敳搠?瑮攠捡桮湡潭汭潯杸椠数獲?晣潥牳?渠楢瑹爠潢条整湣?爠整浥潳癴慳氠???摥癤愠湯据攠獴?楥渠?灩牴潲捯敧獥獮?獧瑡慳爠瑰?畯灤?慣湴摩?牮敛浊慝椮湅楮湺杹?楥猠猦甠敍獩季?嵯??桡敬洠潔獥灣桨敮牯敬??べ???????????????????????樱?挱栶支浪漮獥灮桺敭物散??っ??????????戱爸??孲??嵛″?啝?乪?个??????測慉洮涌漧砨?或愯推瓇攋狊榮感??昽狓澄浹?摛楊獝挮漠皯斃狑祦?琲漰?愷瀬瀳永椨挱愲琩椺漵渱嬸?崭?丱愹琱甼牢敲￣删敛瘳椵敝眠獙??椮挠犌漧戨椧漖泌澄柌秆??ナす????????金???呼???》??????渱爴洼楢捲爾漠?????拦爸??嬠??嵣?圠刘‐噯???十??????????????国?乡??伧且??乊?售????敦琎?愯氬?吰栱收?洳改洨戲爩愺渱攲′戭椱漲爷攼慢捲琾漠牛????湍潏癌敉汎?瑅潖潏氠?琬漠?杁牒潃睉?愠湍愬洠测潁硒?手慁捓瑈故牖椠慄?愠獥?映牡敬攮?据敡汭汭獯學?嵦??椠潡瑭敭捯桮湩潡氠潲来祭????椠潦敲湯杭椠湰敩敧爠業湡杮??づ????ぬ?????呼???????ㄠは?ㄠはひ??扮楩瑣???????戠牣??孴??嵴??卮???????即唠坰??奦?????啣剥??奝??敩瑯?慥汳??湲慣敥爠潔扥楣捨?慯浬浯潧湹椬甲洰‰漹砬椱搰愰琨椷漩渺′?愷渱愭洲洱漷砵??椰爮爱攰瘱收爯獪椮扢汩祯?楴湥档楨戮椲琰攰搸?戱礰?洰攳琸格慢湲漾氠孛?崸??灈灕氠楍攬搠??楎捇爠潘戬椠潗汅潎朠祘????椠潡瑬攮捍桩湣潲汯潢杩祡?㈠っは?????????????????び??の???獦てづ?????ぷ???????づ?戠牴?eatment plants as revealed by 454-pyrosequencing analysis[J].Bioresource Technology,2012,117(10):72-79 10.1016/j.biortech.2012.04.061 |
| [31] | SCHLOSS P D, GEVERS D, WESTCOTT S L.Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies[J].Plos One,2011,6(12):e27310 10.1371/journal.pone.0027310 |
| [32] | WANG Y, BU C N, KANG Q, et al.Autoclaved sludge as the ideal seed to culture anammox bacteria: Reactor performance and microbial community diversity[J].Bioresource Technology,2017,244:391-399 10.1016/j.biortech.2017.07.161 |
| [33] | RIVIéRE D, DESVIGNES V, PELLETIER E, et al.Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge[J].ISME Journal,2009,3(6):700-714 10.1038/ismej.2009.2 |
| [34] | FIERER N, BRADFORD |
Turn off MathJax -->
点击查看大图计量
文章访问数:927
HTML全文浏览数:702
PDF下载数:138
施引文献:0
出版历程
刊出日期:2018-11-29
-->
厌氧氨氧化工艺快速启动策略及其微生物特性
杨瑞丽1,2,王晓君1,
吴俊斌1,
郭焱1,2,
张召基1,
陈少华1
1.中国科学院城市环境研究所，城市污染物转化重点实验室，厦门 361021
2.中国科学院大学，北京 100049
基金项目: 福建省自然科学基金资助项目（2015J05115） 中国科学院城市环境研究所青年前沿项目（IUEMS201404）
关键词: 厌氧氨氧化（anammox）/
生物脱氮/
微生物特性/
快速启动
摘要:为探讨种泥投加及氮负荷提升方式对厌氧氨氧化（anaerobic ammonia oxidation, anammox）工艺启动中微生物丰度及群落结构的影响，采取先普通活性污泥驯化后再接种anammox种泥的方式启动anammox工艺。结果表明在活性迟滞阶段投加anammox菌种可以快速启动anammox工艺。通过缩短水力停留时间的方式增加氮负荷并可以避免直接提高进水氮浓度导致的基质毒性抑制，有利于达到更高的总氮去除负荷。稳定运行时反应器的氮去除负荷达0.51 kg·(m3·d)-1，anammox菌基因丰度为4.92×109 copies·g-1 (以VSS计)，占细菌总数的2.70%。启动阶段，反应器内微生物多样性逐渐下降，检测到浮霉菌门中4个anammox菌属，以Candidatus Jettenia和Candidatus Kuenenia为主要anammox菌属。在接种污泥处于活性迟滞阶段时，结合提高进水氮浓度、缩短水力停留时间和投加anammox菌种的方式可以快速启动anammox工艺。
English Abstract
Rapid start-up strategy and microbial characteristics of anammox process
YANG Ruili1,2,WANG Xiaojun1,
WU Junbin1,
GUO Yan1,2,
ZHANG Zhaoji1,
CHEN Shaohua1
1.Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
Keywords: anaerobic ammonia oxidation (anammox)/
biological removal of nitrogen/
microorganism characteristics/
rapid start-up
Abstract:In order to investigate the effects of seed sludge inoculation and increase mode for the influent nitrogen load on the microbial abundance and community in anaerobic ammonia oxidation (anammox) reactor during a start-up period, the anammox process was initiated by inoculating acclimated activated sludge with anammox seed sludge. Results showed that adding anammox strain at a lag stage was beneficial to the rapid start-up of pilot-scale reactor. An improved nitrogen removal rate (NRR) was achieved through shortening hydraulic retention time as well as increasing the influent nitrogen load, which was an effective means to avoid the inhibition of matrix toxicity caused by increasing the influent TN concentration. In the steady running phase, the NRR of 0.51 kg·(m3·d)-1 was realized, and the gene abundance of anammox bacteria reached 4.92×109 copies·g-1 (calculated by VSS), accounting for 2.70% of the total bacteria. In the start-up phase, the microbial diversity in the reactor gradually decreased, four anammox bacteria genus belonging to Planctomycetes were identified, and the dominant genus of functional bacteria were Candidatus Jettenia and Candidatus Kuenenia. The anammox process was successfully start-up with short term by inoculating anammox seed sludge, raising influent nitrogen and shorting hydraulic retention time at the lag stage of inoculation sludge.
