马清佳^1,2,,
田哲^2,3,
员建¹,
宋建阳²,
杨敏^2,3,
张昱^2,3
1.天津城建大学环境与市政工程学院，天津 300384
2.中国科学院生态环境研究中心环境水质学国家重点实验室，北京 100085
3.中国科学院大学，北京 100049
基金项目: 国家自然科学基金资助项目（21437005，51608513）

Acute inhibition of nine antibiotics on sludge thermophilic anaerobic digestion

MA Qingjia^1,2,,
TIAN Zhe^2,3,
YUAN Jian¹,
SONG Jianyang²,
YANG Min^2,3,
ZHANG Yu^2,3
1.School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China
2.State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences Chinese Academy of Science, Beijing 100085,China
3.University of Chinese Academy of Sciences, Beijing 100049, China

-->

摘要
HTML全文
图(0)表(0)
参考文献(40)
相关文章
施引文献
资源附件(0)
访问统计

摘要:通过急性抑制实验方法评价了9种抗生素（2种氨基糖苷类抗生素核糖霉素、链霉素，2种大环内酯类抗生素红霉素、螺旋霉素，2种四环素类抗生素四环素、土霉素，以及林可霉素、氯霉素和头孢唑啉）在2种浓度(25 mg·L-1和50 mg·L-1)水平下对污泥高温（55 ℃）厌氧消化的急性抑制效果。结果表明，土霉素与链霉素在2种浓度水平下均未对高温厌氧消化产生急性抑制，而四环素与核糖霉素仅在50 mg·L-1水平下导致甲烷累计产量分别下降了36.13%和17.50%。头孢唑啉、林可霉素、红霉素、螺旋霉素和氯霉素在25 mg·L-1浓度水平下即表现出较强的急性抑制效果，其中25 mg·L-1的林可霉素和氯霉素暴露系统中甲烷的累计产量分别下降了29.39%和19.88%，50 mg·L-1浓度水平下进一步降低到51.27%和49.46%。而头孢唑啉、红霉素和螺旋霉素在25 mg·L-1和50 mg·L-1浓度水平下表现出相似的急性抑制效果，累计甲烷产量分别降低了43.03%～47.49%、40.60%～44.91%和54.61%～55.69%。高温厌氧消化前后挥发性有机酸的变化表明，林可霉素主要通过抑制丁酸互营氧化菌活性降低乙酸产量从而降低甲烷产量，50 mg·L-1的核糖霉素主要抑制乙酸型产甲烷古菌活性，而红霉素、螺旋霉素、氯霉素、头孢唑啉和四环素可同时抑制产甲烷古菌和丁酸互营氧化菌活性。
关键词: 高温厌氧消化/
抗生素/
头孢唑啉/
氯霉素/
急性抑制

Abstract:This study evaluated the short-term acute inhibition impacts of nine antibiotics (ribostamycin, streptomycin, erythromycin, spiramycin, tetracycline, oxytetracycline, lincomycin, chloramphenicol, and cefazolin) at two concentration levels (25 mg·L-1 and 50 mg·L-1) on thermophilic anaerobic digestion (AD). Results showed that oxytetracycline and streptomycin at the concentration of 25 mg·L-1and 50 mg·L-1 didn’t inhibited methane production, while tetracycline and ribostamycin at the concentration of 50 mg·L-1 reduced methane production by 36.13% and 17.50%, respectively, after thermophilic AD for seven days. Cefazolin, lincomycin, erythromycin, spiramycin, and chloramphenicol showed a strong acute inhibitory effect at the concentration of 25 mg·L-1. The cumulative methane production in lincomycin and chloramphenicol exposing systems decreased by 29.39% and 19.88%, respectively, at the concentration of 25 mg·L-1, and further decreased to 51.27% and 49.46%, respectively, at the concentration of 50 mg·L-1. Cefazolin, erythromycin and spiramycin showed similar acute inhibitory effects at concentrations of 25 mg·L-1 and 50 mg·L-1, with their cumulative methane productions decreasing by 43.03% to 47.49%, 40.60% to 44.91% and 54.61% to 55.69%, respectively. The change of volatile organic acids after thermophilic AD indicated that lincomycin could reduce methane production by inhibiting syntrophic butyric-acid-oxidizing bacteria, and ribothromycin mainly impacted the activity of aceticlastic methanogen. At the same time, erythromycin, spiramycin, chloramphenicol, cefazolin, and tetracycline at the tested concentrations might inhibit both the activity of methanogen and syntrophic bacteria.
Key words:thermophilic anaerobic digestion/
antibiotic/
cefazolin/
chloramphenicol/
acute inhibition.

[1]	胡献刚，罗义，周启星，等. 固相萃取-高效液相色谱法测定畜牧粪便中13种抗生素药物残留[J]. 分析化学,2008,36(9):1162-1166
[2]	HO Y B, ZAKARIA M P, LATIF P A, et al.Occurrence of veterinary antibiotics and progesterone in broiler manure and agricultural soil in Malaysia[J].Science of the Total Environment,2014,488–489:261-267 10.1016/j.scitotenv.2014.04.109
[3]	SOLLIEC M, MASSE D, SAUVE S.Analysis of trimethoprim, lincomycin, sulfadoxin and tylosin in swine manure using laser diode thermal desorption-atmospheric pressure chemical ionization-tandem massspectrometry[J].Talanta,2014,128:23-30 10.1016/j.talanta.2014.04.023
[4]	HU X G, LUO Y, ZHOU Q X, et al.Determination of thirteen antibiotics residues in manure by solid phase extraction and high performance liquid chromatography[J].Chinese Journal of Analytical Chemistry,2008,36(9):1162-1166 10.1016/S1872-2040(08)60063-8
[5]	LI D, YANG M, HU J, et al.Determination and fate of oxytetracycline and related compounds in oxytetracycline production wastewater and the receiving river[J].Environmental Toxicology and Chemistry,2008,27(1):80-86 10.1897/07-080.1
[6]	LIU M, ZHANG Y, YANG M, et al.Abundance and distribution of tetracycline resistance genes and mobile elements in an oxytetracycline production wastewater treatment system[J].Environmental Science & Technology,2012,46(14):7551-7557 10.1021/es301145m
[7]	LI B, ZHANG T.Biodegradation and adsorption of antibiotics in the activated sludge process[J].Environmental Science & Technology,2010,44(9):3468-3473 10.1021/es903490h
[8]	TANG M, DOU X M, WANG C Y, et al.Abundance and distribution of antibiotic resistance genes in a full-scale anaerobic–aerobic system alternately treating ribostamycin, spiramycin and paromomycin production wastewater[J].Environmental Geochemistry and Health,2017,39:1595-1605 10.1007/s10653-017-9987-5
[9]	PALA-OZKOK I, ORHON D.Chronic effect of erythromycin on substrate biodegradation kinetics of activated sludge[J].Biochemical Engineering Journal,2013,81(4):29-39 10.1016/j.bej.2013.10.002
[10]	CETECIOGLU Z, INCE B, ORHON D, et al.Acute inhibitory impact of antimicrobials on acetoclastic methanogenic activity[J].Bioresource Technology,2012,114:109-116 10.1016/j.biortech.2012.03.020
[11]	何品晶，管冬兴，吴铎，等. 氨氮和林可霉素对有机物厌氧消化的抑制效应[J]. 化工学报,2011,62(5):1389-1394
[12]	余博，田哲，池勇志，等. 土霉素对剩余污泥中温厌氧消化的短期和长期影响[J]. 环境工程学报,2016,10(4):2009-2015 10.12030/j.cjee.20160469
[13]	LIN H, ZHANG J, CHEN H, et al.Effect of temperature on sulfonamide antibiotics degradation, and on antibiotic resistance determinants and hosts in animal manures[J].Science of the Total Environment,2017,607:725-732 10.1016/j.scitotenv.2017.07.057
[14]	LOFTIN K A, ADAMS C D, MEYER M T, et al.Effects of ionic strength, temperature, and pH on degradation of selected antibiotics[J].Journal of Environmental Quality,2008,37(2):378-386 10.2134/jeq2007.0230
[15]	ALMEIDA S D.Comparison of the anaerobic digestion at the mesophilic and thermophilic temperature regime of organic wastes from the agribusiness[J].Bioresource Technology,2017,241:985-992 10.1016/j.biortech.2017.06.006.
[16]	JANG H M, SHIN J, CHOI S, et al.Fate of antibiotic resistance genes in mesophilic and thermophilic anaerobic digestion of chemically enhanced primary treatment (CEPT) sludge[J].Bioresource Technology,2017,244(Part 1):433-444 10.1016/j.biortech.2017.07.153
[17]	WITHEY J M, MUGO S M, ZHOU T, et al.Depletion of hormones and antimicrobials in cattle manure using thermophilic anaerobic digestion[J].Journal of Chemical Technology and Biotechnology,2016,91(9):2404-2411 10.1002/jctb.4823
[18]	王翀. 提高香蕉（Musa sapientumLinn）秸秆厌氧消化性能的预处理方法及工艺研究[D]. 长沙:湖南农业大学,2015
[19]	贺延龄. 废水的厌氧生物处理[M]. 北京:中国轻工业出版社,1998.
[20]	陈峻峰，颜智勇，邵继海，等. 金霉素对畜禽养殖废水厌氧生物处理及细菌多样性的影响[J]. 净水技术,2014,33(2):76-80
[21]	崔凤杰，李向菲，周宇光，等.NaOH预处理对玉米秸秆固态厌氧消化的影响[J]. 环境工程学报,2013,7(5):1919-1924
[22]	INCE O, KOLUKIRIK M, CETECIOGLU Z, et al.Toluene inhibition on an anaerobic reactor sludge in terms of potential activity and composition of acetoclastic methanogens[J].Environmental Letters,2009,44(14):1551-1556 10.1080/10934520903263470
[23]	INCE O, KOLUKIRIK M, CETECIOGLU Z, et al.Methanogenic and sulphate reducing bacterial population levels in a full-scale anaerobic reactor treating pulp and paper industry wastewater using fluorescence in situ hybridisation[J].Water Science and Technology,2007,55(10):183-191 10.2166/wst.2007.321
[24]	GRIFFIN M E, MCMAHON K D, MACKIE R I, et al.Methanogenic population dynamics during start-up of anaerobic digesters treating municipal solid waste and biosolids[J].Biotechnology and Bioengineering,1998,57(3):342-355 10.1002/(SICI)1097-0290(19980205)57:33.0.CO;2-I
[25]	TIAN Z, ZHANG Y, LI Y Y, et al.Rapid establishment of thermophilic anaerobic microbial community during the one-step startup of thermophilic anaerobic digestion from a mesophilic digester[J].Water Research,2015,69:9-19 10.1016/j.watres.2014.11.001
[26]	MCCARTY P L, MOSEY F E.Modeling of anaerobic-digestion processes (a discussion of concepts)[J].Water Science and Technology,1991,24(8):17-33
[27]	BOUSKOVA A, DOHANYOS M, SCHMIDT J E, et al.Strategies for changing temperature from mesophilic to thermophilic conditions in anaerobic CSTR reactors treating sewage sludge[J].Water Research,2005,39(8):1481-1488 10.1016/j.watres.2004.12.042
[28]	YI Q Z, GAO Y X, ZHANG H, et al.Establishment of a pretreatment method for tetracycline production wastewater using enhanced hydrolysis[J].Chemical Engineering Journal,2016,300:139-145 10.1016/j.cej.2016.04.120
[29]	AKYOL C, TURKER G, INCE O, et al.Performance and microbial community variations in thermophilic anaerobic digesters treating OTC medicated cow manure under different operational conditions[J].Bioresource Technology,2016,205:191-198 10.1016/j.biortech.2016.01.071
[30]	CHOPRA I, ROBERTS M.Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance[J].Microbiology and Molecular Biology Reviews,2001,65(2):232-260 10.1128/MMBR.65.2.232-260.2001
[31]	LAMBERTI A, MARTUCCI N M, RUGGIERO I, et al.Interaction between the antibiotic tetracycline and the elongation factor 1α from the archaeon Sulfolobus solfataricus[J].Chemical Biology & Drug Design,2011,78(2):260–268 10.1111/j.1747-0285.2011.01142.x
[32]	CAMMARANO P, TEICHNER A, LONDEI P, et al.Insensitivity of archaebacterial ribosomes to protein synthesis inhibitors.Evolutionary implications[J].EMBO Journal,1985,4(3):811-816
[33]	BENERAGAMA N, IWASAKI M, LATEEF S A, et al.The effect of cefazolin on biogas production from thermophilic and mesophilic anaerobic co-digestion of dairy manure and waste milk[J].Journal of National Science Foundation of Sri Lanka,2015,43(4):369-376 10.4038/jnsfsr.v43i4.7971
[34]	BENERAGAMA N, LATEEF S A, IWASAKI M, et al.The combined effect of cefazolin and oxytertracycline on biogas production from thermophilic anaerobic digestion of dairy manure[J].Bioresource Technology,2013,133(4):23-30 10.1016/j.biortech.2013.01.032
[35]	陈兆坤，胡昌勤. 头孢菌素类抗生素的降解机制[J]. 国外医药抗生素分册,2004,25(6):249-252
[36]	KHELAIFIA S, DRANCOURT M.Susceptibility of archaea to antimicrobial agents: applications to clinical microbiology[J].Clinical Microbiology and Infection,2012,18(9):841-848 10.1111/j.1469-0691.2012.03913.x
[37]	HILPERT R, WINTER J, HAMMES W, et al.The sensitivity of archaebacteria to antibiotics[J].Zentralblatt Für Bakteriologie Und Hygiene Reihe C,1981,2(1):11-20 10.1016/S0721-9571(81)80014-2
[38]	马文成. 水解酸化-两级厌氧工艺处理甲醇废水的试验研究[D]. 哈尔滨:哈尔滨工业大学,2008
[39]	祖波，祖建，周富春，等. 产甲烷菌的生理生化特性[J]. 环境科学与技术,2008,31(3):5-8
[40]	MCKELLAR R C, SPROTT G D.Solubilization and properties of a particulate hydrogenase from Methanobacterium strain G2R[J].Journal of Bacteriology,1979,139(1):231-238

PDF下载( 3268 KB)XML下载导出引用Turn off MathJax -->
点击查看大图

计量

文章访问数:802
HTML全文浏览数:561
PDF下载数:247
施引文献:0

出版历程

刊出日期:2018-07-26

---

-->

9种抗生素对污泥高温厌氧消化的急性抑制

马清佳^1,2,,
田哲^2,3,
员建¹,
宋建阳²,
杨敏^2,3,
张昱^2,3
1.天津城建大学环境与市政工程学院，天津 300384
2.中国科学院生态环境研究中心环境水质学国家重点实验室，北京 100085
3.中国科学院大学，北京 100049
基金项目: 国家自然科学基金资助项目（21437005，51608513）
关键词: 高温厌氧消化/
抗生素/
头孢唑啉/
氯霉素/
急性抑制
摘要:通过急性抑制实验方法评价了9种抗生素（2种氨基糖苷类抗生素核糖霉素、链霉素，2种大环内酯类抗生素红霉素、螺旋霉素，2种四环素类抗生素四环素、土霉素，以及林可霉素、氯霉素和头孢唑啉）在2种浓度(25 mg·L-1和50 mg·L-1)水平下对污泥高温（55 ℃）厌氧消化的急性抑制效果。结果表明，土霉素与链霉素在2种浓度水平下均未对高温厌氧消化产生急性抑制，而四环素与核糖霉素仅在50 mg·L-1水平下导致甲烷累计产量分别下降了36.13%和17.50%。头孢唑啉、林可霉素、红霉素、螺旋霉素和氯霉素在25 mg·L-1浓度水平下即表现出较强的急性抑制效果，其中25 mg·L-1的林可霉素和氯霉素暴露系统中甲烷的累计产量分别下降了29.39%和19.88%，50 mg·L-1浓度水平下进一步降低到51.27%和49.46%。而头孢唑啉、红霉素和螺旋霉素在25 mg·L-1和50 mg·L-1浓度水平下表现出相似的急性抑制效果，累计甲烷产量分别降低了43.03%～47.49%、40.60%～44.91%和54.61%～55.69%。高温厌氧消化前后挥发性有机酸的变化表明，林可霉素主要通过抑制丁酸互营氧化菌活性降低乙酸产量从而降低甲烷产量，50 mg·L-1的核糖霉素主要抑制乙酸型产甲烷古菌活性，而红霉素、螺旋霉素、氯霉素、头孢唑啉和四环素可同时抑制产甲烷古菌和丁酸互营氧化菌活性。

English Abstract

全文HTML

--> --> --> 参考文献 (40)