王成燚1,2,
汪跃1,2,
陈琳1,2
1.河海大学环境学院，南京 210098
2.河海大学浅水湖泊综合治理与资源开发教育部重点实验室，南京 210098
基金项目: 国家自然科学基金资助项目（51508153）
江苏省自然科学基金资助项目（BK20150813）
Effect of ZnO nanoparticles on production and membrane fouling performance of biomass-associated products in anaerobic sludge
HU Qinzheng1,2,,WANG Chengyi1,2,
WANG Yue1,2,
CHEN Lin1,2
1.College of Enviroment, Hohai University, Nanjing 210098, China
2.Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China
-->
摘要
HTML全文
图
参考文献
相关文章
施引文献
资源附件
访问统计
摘要:从厌氧污泥的角度，探究ZnO-NPs对微生物内源代谢产物(BAP)产量和膜污染性能的影响。在实验室培养的厌氧污泥中投加不同浓度的ZnO-NP，通过序批式实验和过滤实验，分别考察ZnO-NP作用下BAP的产量和膜污染性能的变化规律。发现在ZnO-NPs的短期暴露下，BAP和胞外聚合物(EPS)的总量都随着ZnO-NPs浓度的增加而增加。当ZnO-NPs浓度为300 mg·L-1时，BAP和EPS的7 d总产量相较于空白对照组，增幅分别为303.4%和112%，远远高于其他实验组。过滤实验发现，随着ZnO-NPs的增加，BAP的膜污染性能不断增加。此外，通过扫描电镜(SEM)和三维荧光光谱(EEM)分别对污泥形态和BAP特性进行研究，发现在ZnO-NPs短期暴露下，ZnO-NPs的浓度越高，其对厌氧污泥的形态和BAP特性影响越大。
关键词: ZnO-NPs/
BAP/
EPS/
SEM/
EEM/
膜污染性能
Abstract:The purpose of this study was to research the influence of ZnO-NPs on the production of biomass-associated products (BAP) in anaerobic sludge and its membrane fouling potential. Different concentrations of ZnO-NP were added in the anaerobic sludge cultured in laboratory. The change of BAP production under ZnO-NP dosage was investigated by sequencing batch reactor, and the membrane fouling potential was evaluated through dead-end filtration expeirments. The content of BAP and extracellular polymeric substances (EPS) increased with the increasing dosage of ZnO-NPs during 7 days experiments. When the concentration of ZnO-NPs was 300 mg·L-1, the increments of BAP and EPS were up to 303.4% and 112%, respectively, which was much higher than that of other experimental groups. Filtration experiments showed that the water flux for each experimental group decreased with the increase of ZnO-NPs concentration. Additionally, the morphology of sludge and BAP characteristics were studied by scanning electron microscopy (SEM) and three-dimensional fluorescence spectroscopy (EEM), respectively. Greater impact on the morphology of anaerobic sludge and BAP characteristics was found under higher ZnO-NPs concentration.
Key words:ZnO-NPs/
BAP/
EPS/
SEM/
EEM/
membrane fouling.
| [1] | KOLODZIEJCZAK-RADZIMSKA A, JESIONOWSKI T.Zinc oxide-from synthesis to application: A review[J].Materials,2014,7(4):2833-2881 10.3390/ma7042833 |
| [2] | XIONG H M.ZnO nanoparticles applied to bioimaging and drug delivery[J].Advanced Materials,2013,25(37):5329-5335 |
| [3] | KHATAEE A, KARIMI A, AREFI-OSKOUI S, et al.Sonochemical synthesis of Pr-doped ZnO nanoparticles for sonocatalytic degradation of acid red 17[J].Ultrasonics Sonochemistry,2015,22:371-381 10.1016/j.ultsonch.2014.05.023 |
| [4] | MA H, WILLIAMS P L, DIAMOND S A.Ecotoxicity of manufactured ZnO nanoparticles: A review[J].Environmental Pollution,2013,172(1):76–85 10.1016/j.envpol.2012.08.011 |
| [5] | GOTTSCHALK F, SONDERER T, SCHOLZ R W, et al.Modeled environmental concentrations of engineered nanomaterials (TiO2, ZnO, Ag, CNT, Fullerenes) for different regions[J].Environmental Science & Technology,2009,43(24):9216-9222 |
| [6] | 马学文, 翁焕新, 章金骏. 中国城市污泥重金属和养分的区域特性及变化[J]. 中国环境科学,2011,31(8):1306-1313 |
| [7] | 杨军, 郭广慧, 陈同斌, 等. 中国城市污泥的重金属含量及其变化趋势[J]. 中国给水排水,2009,25(13):122-124 |
| [8] | PUAY N Q, QIU G, TING Y P.Effect of zinc oxide nanoparticles on biological wastewater treatment in a sequencing batch reactor[J].Journal of Cleaner Production,2015,88:139-145 10.1016/j.jclepro.2014.03.081 |
| [9] | HOU J, MIAO L, WANG C, et al.Inhibitory effects of ZnO nanoparticles on aerobic wastewater biofilms from oxygen concentration profiles determined by microelectrodes[J].Journal of Hazardous Materials,2014,276:164-170 10.1016/j.jhazmat.2014.04.048 |
| [10] | ZHANG D, ENG C Y, STUCKEY D, et al.Effects of ZnO nanoparticle exposure on wastewater treatment and soluble microbial products (SMPs) in an anoxic-aerobic membrane bioreactor[J].Chemosphere,2017,171:446-459 10.1016/j.chemosphere.2016.12.053 |
| [11] | LOMBI E, DONNER E, TAVAKKOLI E, et al.Fate of zinc oxide nanoparticles during anaerobic digestion of wastewater and post-treatment processing of sewage sludge[J].Environmental Science & Technology,2012,46(16):9089-9096 |
| [12] | ZHENG X, WU R, CHEN Y.Effects of ZnO nanoparticles on wastewater biological nitrogen and phosphorus removal[J].Environmental Science & Technology,2011,45(7):2826-2832 |
| [13] | WANG Z P, ZHANG T.Characterization of soluble microbial products (SMP) under stressful conditions[J].Water Research,2010,44(18):5499-5509 10.1016/j.watres.2010.06.067 |
| [14] | KUNACHEVA C, SOH Y N A, STUCKEY D C.Effect of feed pH on reactor performance and production of soluble microbial products (SMPs) in a submerged anaerobic membrane bioreactor[J].Chemical Engineering Journal,2017,320:135-143 10.1016/j.cej.2017.03.018 |
| [15] | AVELLA A C, DELGADO L F, GORNER T, et al.Effect of cytostatic drug presence on extracellular polymeric substances formation in municipal wastewater treated by membrane bioreactor[J].Bioresource Technology,2010,101(2):518-526 10.1016/j.biortech.2009.08.057 |
| [16] | HAN J C, LIU Y, LIU X, et al.The effect of continuous Zn (II) exposure on the organic degradation capability and soluble microbial products (SMP) of activated sludge[J].Journal of Hazardous Materials,2013,244-245(2):489-494 10.1016/j.jhazmat.2012.10.065 |
| [17] | LASPIDOU C S, RITTMANN B E.A unified theory for extracellular polymeric substances, soluble microbial products, and active and inert biomass[J].Water Research,2002,36(11):2711-2720 10.1016/S0043-1354(01)00413-4 |
| [18] | MEI X, WANG Z, XIANG Z, et al.Soluble microbial products in membrane bioreactors in the presence of ZnO nanoparticles[J].Journal of Membrane Science,2014,451(1):169-176 10.1016/j.memsci.2013.10.008 |
| [19] | NI B J, RITTMZNN B E, YU H Q.Soluble microbial products and their implications in mixed culture biotechnology[J].Trends in Biotechnology,2011,29(9):454-463 10.1016/j.tibtech.2011.04.006 |
| [20] | LIANG Z, LI W, YANG S, et al.Extraction and structural characteristics of extracellular polymeric substances (EPS), pellets in autotrophic nitrifying biofilm and activated sludge[J].Chemosphere,2010,81(5):626-632 10.1016/j.chemosphere.2010.03.043 |
| [21] | LOWRY O H, ROSEBROUGH N J, FARR A L, et al.Protein measurement with the folin phenol reagent[J].Journal of Biological Chemistry,1951,193(1):265-275 |
| [22] | DUBOIS M, GILLES K A, HAMILTON J K, et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry,1955,28(3):350-356 10.1021/ac60111a017 |
| [23] | 王子超. 盐度和重金属对序批式生物反应器性能及微生物群落结构影响的研究[D]. 青岛：中国海洋大学,2014 |
| [24] | 吴杨芳, 冯骞, 薛朝霞, 等. 纳米氧化铈作用下活性污泥胞外聚合物及溶解性微生物产物特性[J]. 微生物学通报,2016,43(2):262-269 |
| [25] | LIU Y, FANG H H P.Influences of extracellular polymeric substances (EPS) on flocculation, settling, and dewatering of activated sludge[J].Critical Reviews in Environmental Science and Technology,2003,33(3):237-273 10.1080/10643380390814479 |
| [26] | CHEN W, WESTERHOFF P, LEENHEER J A, et al.Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter[J].Environmental Science & Technology,2003,37(24):5701-5710 |
| [27] | CHEN J, GU B, LEBOEUF E J, et al.Spectroscopic characterization of the structural and functional properties of natural organic matter fractions[J].Chemosphere,2002,48(1):59-68 10.1016/S0045-6535(02)00041-3 |
| [28] | WEI L, YANG L, NOGUERA D R, et al.Adsorption of Cu2+, and Zn2+, by extracellular polymeric substances (EPS) in different sludges: Effect of EPS fractional polarity on binding mechanism[J].Journal of Hazardous Materials,2017,321:473-483 10.1016/j.jhazmat.2016.05.016 |
| [29] | 于晓霞. 膜生物反应器中胞外聚合物与膜污染关系的研究[D]. 北京：北京交通大学,2008 |
| [30] | HREMIA J.Constant pressure blocking laws application to power-law non-newtonian fluids[J].Transactions Institute of Chemical Engineers,1982,60:183-187 |
Turn off MathJax -->
点击查看大图计量
文章访问数:1427
HTML全文浏览数:976
PDF下载数:383
施引文献:0
出版历程
刊出日期:2018-06-18
-->
纳米氧化锌对厌氧污泥中微生物内源代谢产物和膜污染性能的影响
胡勤政1,2,,王成燚1,2,
汪跃1,2,
陈琳1,2
1.河海大学环境学院，南京 210098
2.河海大学浅水湖泊综合治理与资源开发教育部重点实验室，南京 210098
基金项目: 国家自然科学基金资助项目（51508153） 江苏省自然科学基金资助项目（BK20150813）
关键词: ZnO-NPs/
BAP/
EPS/
SEM/
EEM/
膜污染性能
摘要:从厌氧污泥的角度，探究ZnO-NPs对微生物内源代谢产物(BAP)产量和膜污染性能的影响。在实验室培养的厌氧污泥中投加不同浓度的ZnO-NP，通过序批式实验和过滤实验，分别考察ZnO-NP作用下BAP的产量和膜污染性能的变化规律。发现在ZnO-NPs的短期暴露下，BAP和胞外聚合物(EPS)的总量都随着ZnO-NPs浓度的增加而增加。当ZnO-NPs浓度为300 mg·L-1时，BAP和EPS的7 d总产量相较于空白对照组，增幅分别为303.4%和112%，远远高于其他实验组。过滤实验发现，随着ZnO-NPs的增加，BAP的膜污染性能不断增加。此外，通过扫描电镜(SEM)和三维荧光光谱(EEM)分别对污泥形态和BAP特性进行研究，发现在ZnO-NPs短期暴露下，ZnO-NPs的浓度越高，其对厌氧污泥的形态和BAP特性影响越大。
English Abstract
Effect of ZnO nanoparticles on production and membrane fouling performance of biomass-associated products in anaerobic sludge
HU Qinzheng1,2,,WANG Chengyi1,2,
WANG Yue1,2,
CHEN Lin1,2
1.College of Enviroment, Hohai University, Nanjing 210098, China
2.Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China
Keywords: ZnO-NPs/
BAP/
EPS/
SEM/
EEM/
membrane fouling
Abstract:The purpose of this study was to research the influence of ZnO-NPs on the production of biomass-associated products (BAP) in anaerobic sludge and its membrane fouling potential. Different concentrations of ZnO-NP were added in the anaerobic sludge cultured in laboratory. The change of BAP production under ZnO-NP dosage was investigated by sequencing batch reactor, and the membrane fouling potential was evaluated through dead-end filtration expeirments. The content of BAP and extracellular polymeric substances (EPS) increased with the increasing dosage of ZnO-NPs during 7 days experiments. When the concentration of ZnO-NPs was 300 mg·L-1, the increments of BAP and EPS were up to 303.4% and 112%, respectively, which was much higher than that of other experimental groups. Filtration experiments showed that the water flux for each experimental group decreased with the increase of ZnO-NPs concentration. Additionally, the morphology of sludge and BAP characteristics were studied by scanning electron microscopy (SEM) and three-dimensional fluorescence spectroscopy (EEM), respectively. Greater impact on the morphology of anaerobic sludge and BAP characteristics was found under higher ZnO-NPs concentration.
