郑晓英¹,
乔露露¹,
王慰²,
李楠¹,
李魁晓²
1.北京工业大学北京市水质科学与水环境恢复工程重点实验室，北京100124
2.北京城市排水集团有限责任公司科技研发中心，北京100022

Effects of carbon sources on operation and microbial population of denitrification biological filter

ZHENG Xiaoying¹,
QIAO Lulu¹,
WANG Wei²,
LI Nan¹,
LI Kuixiao²
1.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
2.Research and Development Center of Beijing Drainage Group Co.Ltd.,Beijing 100022, China

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摘要:以某城市污水厂二级出水为原水，以甲醇、乙酸钠为碳源，研究了不同碳源对反硝化生物滤池运行的影响，并借助16S rDNA测序技术对滤池生物膜的微生物群落组成和结构进行了解析。结果表明，采用逐渐增加滤速的方式进行挂膜，乙酸钠滤池在启动7 d后出水水质稳 定，NO3--N去除率在96%以上，NO2--N积累消失；甲醇滤池则需要9 d。稳定运行期，甲醇和乙酸钠滤池达到最大反硝化效率所需碳氮比均为4.5～5.5，出水TN＜1.0 mg·L-1。乙酸钠滤池沿过滤方向硝酸盐氮降解较快。与甲醇相比，乙酸钠微生物产量高、运行周期短、反冲洗时间长，且药剂投加量高。从滤池脱氮效率、运行稳定性和成本等方面综合考虑，甲醇可作为最佳碳源。微生物在属水平进行聚类分析结果表明，以甲醇、乙酸钠为碳源的反硝化生物滤池中的微生物种群存在差异。甲醇滤池中与反硝化有关的属占36.68%，其中优势菌属Methylophilus，属于嗜甲基型菌属。乙酸钠滤池中与反硝化有关的菌属占58.38%。其优势菌属为Arobacter，可利用有机酸还原硝酸盐。
关键词: 脱氮/
反硝化滤池/
甲醇/
乙酸钠/
16S rDNA/
微生物群落

Abstract:The effects of different additional carbon sources on the operation of the denitrification biofilter were studied, using the secondary effluent from a municipal wastewater treatment plant as raw water. Methanol and sodium acetate were selected as carbon sources. The composition and structure of microbial community of biofilm in the biofilter were analyzed by 16S rDNA. The results showed that when hanging biofilm by gradually increased filter speed, the water quality was stable with the removal efficiency for NO3--N being over 96%. The accumulation of NO2--N disappeared after 7 days of operation for the filter using sodium acetate as carbon source. However, 9 days were needed for disappeared accumulation of NO2--N in the methanol filter. During the stable operating period, the ratios of carbon to nitrogen required for maximum denitrification efficiency were from 4.5 to 5.5, and the effluent TN was less than 1.0 mg·L-1 in both filters. Compared with methanol filter, nitrate nitrogen degraded faster along the filter direction in sodium acetate filter, which had higher yield of microorganisms, shorter running period, longer backwash time and higher chemical reagent investment. With a comprehensive consideration of the filter denitrification efficiency, operating stability and cost considerations, methanol was better than sodium acetate as carbon source. Moreover, the results of microflora clustering analysis showed that there was a significant difference in microbial community between the above investigated biofilters. The denitrification-related genera in the methanol filter accounted for 36.68%, and the dominant bacterium was Methylophilus, which is methyl-bacterium. The denitrification-related genera in the sodium acetate filter accounted for 58.38%, and the dominant bacterium was Arobacter, which can reduce nitrate through using organic acids.
Key words:nitrogen removal/
denitrification biofilter/
methanol/
sodium acetate/
16S rDNA/
microbial community.

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刊出日期:2018-05-19

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碳源对反硝化生物滤池运行及微生物种群的影响

郑晓英¹,
乔露露¹,
王慰²,
李楠¹,
李魁晓²
1.北京工业大学北京市水质科学与水环境恢复工程重点实验室，北京100124
2.北京城市排水集团有限责任公司科技研发中心，北京100022
基金项目:
关键词: 脱氮/
反硝化滤池/
甲醇/
乙酸钠/
16S rDNA/
微生物群落
摘要:以某城市污水厂二级出水为原水，以甲醇、乙酸钠为碳源，研究了不同碳源对反硝化生物滤池运行的影响，并借助16S rDNA测序技术对滤池生物膜的微生物群落组成和结构进行了解析。结果表明，采用逐渐增加滤速的方式进行挂膜，乙酸钠滤池在启动7 d后出水水质稳 定，NO3--N去除率在96%以上，NO2--N积累消失；甲醇滤池则需要9 d。稳定运行期，甲醇和乙酸钠滤池达到最大反硝化效率所需碳氮比均为4.5～5.5，出水TN＜1.0 mg·L-1。乙酸钠滤池沿过滤方向硝酸盐氮降解较快。与甲醇相比，乙酸钠微生物产量高、运行周期短、反冲洗时间长，且药剂投加量高。从滤池脱氮效率、运行稳定性和成本等方面综合考虑，甲醇可作为最佳碳源。微生物在属水平进行聚类分析结果表明，以甲醇、乙酸钠为碳源的反硝化生物滤池中的微生物种群存在差异。甲醇滤池中与反硝化有关的属占36.68%，其中优势菌属Methylophilus，属于嗜甲基型菌属。乙酸钠滤池中与反硝化有关的菌属占58.38%。其优势菌属为Arobacter，可利用有机酸还原硝酸盐。

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