郑向阳^1,,
罗晓²,
袁立霞¹,
张立国³,
赵丛丛¹
1.河北科技大学建筑工程学院， 石家庄 050018
2.河北科技大学环境科学与工程学院， 石家庄 050018
3.山西大学环境与资源学院， 太原 030006
基金项目: 国家自然科学基金资助项目 (51508316)

Performance and bacterial community analysis of AO process treating starch wastewater

ZHENG Xiangyang^1,,
LUO Xiao²,
YUAN Lixia¹,
ZHANG Liguo³,
ZHAO Congcong¹
1.School of Civil Engineering, Hebei University of Science & Technology, Shijiazhuang 050018, China
2.School of Environmental Science and Engineering, Hebei University of Science & Technology, Shijiazhuang 050018, China
3.College of Environment and Resource, Shanxi University, Taiyuan 030006, China

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摘要:对某淀粉污水厂与实验室的AO处理工艺进行比较研究，通过常规水质监测及Miseq测序技术解析水质处理效果、进水成分差异与AO工艺菌群结构的相关性。结果表明，当进水COD和NH3-N浓度分别为500和450.0 mg·L-1，污水厂稳定运行出水COD和NH3-N浓度分别为83和1.3 mg·L-1，其去除率分别达到96.52%和99.66%；实验室AO处理工艺调试45 d后出水COD和NH3-N浓度分别为78.8和49.9 mg·L-1，其去除率分别达到90.83%和88.50%。Miseq测序结果表明，Proteobacteria、Bacteroidetes、Chloroflexi、Firmicutes、Actinobacteria和Candidate division TM7在各样本中为主要菌门。污水厂与实验室AO处理工艺各样品菌属表现出相同的变化趋势，当AO系统运行良好时，其优势菌属为Anaerolineaceae、Saprospiraceae和Betaproteobacteria等，三者总相对丰度比例为30%。污水厂与实验室AO处理工艺菌群也存在一定差异，其中较显著的有Firmicutes、Saprospiraceae和Betaproteobacteria等。
关键词: AO工艺/
淀粉工业废水/
微生物群落结构/
高通量测序技术/
反应器

Abstract:The microbial community in AO treatment process were investigated and compared between starch wastewater treatment plant and lab scale equipment by Miseq technique. The effects of water quality and treatment factors on microbial community were also explored. The results showed that when the COD and NH3-N concentrations of the influent were 500 and 450.0 mg·L-1, respectively, the COD and NH3-N concentrations in effluent of the starch wastewater treatment plant were 83 and 1.3 mg·L-1, respectively, of which indicated that the COD and NH3-N removal rates were reaching to 96.52% and 99.66%, respectively. For the lab scale equipment, after 45 days of operation, the COD and NH3-N concentrations in effluent were 78.8 and 49.9 mg·L-1, respectively, of which indicated that the COD and NH3-N removal rates were reaching to 90.83% and 88.50%, respectively. Meanwhile, Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Actinobacteria and Candidate division TM7 were the major phylum in each sample, and the same trend was observed in wastewater treatment plant and of the lab scale equipment. Anaerolineaceae, Saprospiraceae and Betaproteobacteria were the dominant species, of which the total relative abundance amount was reaching to 30%. The microorganism community was different between wastewater treatment station and simulated reactor, in which the relative obvious species were Firmicutes, Saprospiraceae and Betaproteobacteria.
Key words:AO process/
starch industrial wastewater/
microbial community structure/
high-throughput sequencing technology/
reactor.

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AO工艺处理淀粉污水效能及微生物群落解析

郑向阳^1,,
罗晓²,
袁立霞¹,
张立国³,
赵丛丛¹
1.河北科技大学建筑工程学院， 石家庄 050018
2.河北科技大学环境科学与工程学院， 石家庄 050018
3.山西大学环境与资源学院， 太原 030006
基金项目: 国家自然科学基金资助项目 (51508316)
关键词: AO工艺/
淀粉工业废水/
微生物群落结构/
高通量测序技术/
反应器
摘要:对某淀粉污水厂与实验室的AO处理工艺进行比较研究，通过常规水质监测及Miseq测序技术解析水质处理效果、进水成分差异与AO工艺菌群结构的相关性。结果表明，当进水COD和NH3-N浓度分别为500和450.0 mg·L-1，污水厂稳定运行出水COD和NH3-N浓度分别为83和1.3 mg·L-1，其去除率分别达到96.52%和99.66%；实验室AO处理工艺调试45 d后出水COD和NH3-N浓度分别为78.8和49.9 mg·L-1，其去除率分别达到90.83%和88.50%。Miseq测序结果表明，Proteobacteria、Bacteroidetes、Chloroflexi、Firmicutes、Actinobacteria和Candidate division TM7在各样本中为主要菌门。污水厂与实验室AO处理工艺各样品菌属表现出相同的变化趋势，当AO系统运行良好时，其优势菌属为Anaerolineaceae、Saprospiraceae和Betaproteobacteria等，三者总相对丰度比例为30%。污水厂与实验室AO处理工艺菌群也存在一定差异，其中较显著的有Firmicutes、Saprospiraceae和Betaproteobacteria等。

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