中文关键词砂滤池微生物群落微量污染物宏基因组降解途径 英文关键词sand filtersmicrobial communitymicropollutantsmetagenomicbiodegradation pathway

作者	单位	E-mail
周洁	中国科学院生态环境研究中心饮用水科学与技术重点实验室, 北京 100085 中国科学院大学资源与环境学院, 北京 100049	jiezhou_st@rcees.ac.cn
王东麟	中国科学院生态环境研究中心饮用水科学与技术重点实验室, 北京 100085 中国科学院大学资源与环境学院, 北京 100049
林慧	中国科学院生态环境研究中心饮用水科学与技术重点实验室, 北京 100085 中国科学院大学资源与环境学院, 北京 100049
柏耀辉	中国科学院大学资源与环境学院, 北京 100049	yhbai@rcees.ac.cn

中文摘要 砂滤池已广泛应用于饮用水处理中，已有研究证明砂滤池能有效降解部分微量污染物，但对其降解途径和降解微生物缺乏深入探讨.据此，本研究首先采集2个实际饮用水厂滤池中的石英砂和锰砂滤料，通过宏基因组分析了滤料对阿替洛尔、阿特拉津、磺胺嘧啶和卡马西平的降解基因和相关降解微生物.结果表明，锰砂滤池生丝微菌科和石英砂滤池中的假单胞菌属具有将阿特拉津转化为羟基阿特拉津的潜力；砂滤池含多种分泌阿替洛尔酰胺水解酶的微生物，具有将阿替洛尔转换为阿替洛尔酸的能力；在滤池中广泛存在单、双加氧酶和细胞色素P450，能将阿特拉津、磺胺嘧啶和卡马西平氧化.进一步通过培养实验验证了滤料对这4种微量污染物的降解途径.本研究最后对其它9个实际水厂砂滤池滤料的宏基因组分析发现所有砂滤池都含有丰富的酰胺水解酶、加氧酶和细胞色素P450，说明饮用水砂滤池都具有降解多种微量污染物的潜力. 英文摘要 Sand filters have been widely used in drinking water treatments. Previous studies have demonstrated that sand filters can degrade some micropollutants. However, the degradation pathway and associated microorganisms have not been clearly investigated. Accordingly, in this study, the quartz sands and manganese sands from two drinking water treatment plants were sampled, and their biodegradation genes for four micropollutants (atrazine, atenolol, sulfadiazine, and carbamazepine) and associated microbial strains were analyzed. Metagenomic results showed that the bacterial strains affiliated with Hyphomicrobiaceae in the manganese sand filter and Pseudomonas strains in the quartz sand filter can transform the atrazine into atrazine-2-hydroxy; atenolamide hydrolase of some microorganisms in the two sand filters can transform atenolol into atenolol acid; and monooxygenase, dioxygenase and cytochrome P450, which can oxidize atrazine, sulfadiazine, and carbamazepine, were widely present in the two sand filters. The study further confirmed the biodegradation pathways of these four micropollutants according to the measurements of transformation intermediates in the cultivation experiment. Finally, the study analyzed the biodegradation genes in the other nine sand filters of drinking water treatment plants and found that abundant amidohydrolase, oxygenase, and cytochrome P450 were present in all sand filters, indicating that sand filters have the potential to biodegrade a number of micropollutants.

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