常叶倩¹,
顾翔¹,
乔宇¹,
陈鸿程¹,
李梅^1,2
1. 南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023;
2. 南京大学环境学院, 环境科学与工程国家级实验教学示范中心, 南京 210023

作者简介: 常叶倩(1995-),女,硕士研究生,研究方向为环境生物学,E-mail:814910081@qq.com.

基金项目: 江苏省科技支撑项目(BE2016736)；国家自然科学基金面上项目(41773115，41571468)；南京大学“十三五”实验教学改革研究重点课题资助项目(SY201911)


中图分类号: X171.5

Toxic Effects of Different Sized Particles on Model Organisms in Drinking Water

Chang Yeqian¹,
Gu Xiang¹,
Qiao Yu¹,
Chen Hongcheng¹,
Li Mei^1,2
1. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China;
2. State Experimental Teaching Demonstration Centre for Environmental Science and Engineering, School of the Environment, Nanjing University, Nanjing 210023, China

CLC number: X171.5

-->

摘要
HTML全文
图(0)表(0)
参考文献(36)
相关文章
施引文献
资源附件(0)
访问统计

摘要:悬浮颗粒物在自然水体中广泛存在，大量小颗粒物(粒径<1 μm)可吸附、聚集水中多种污染物，从而形成新的复合污染，影响水生生态系统。已有研究表明，颗粒物粒径越小，传统饮用水处理工艺越难以去除，因此，其表面吸附的污染物也会随之残留在水体中，对饮用水安全产生影响。本研究对南京某自来水厂进水和出水中的颗粒物进行了测定和对比，并选用3种不同种类的模式生物包括发光菌(Photobacterium phosphoreum)、纤细裸藻(Euglena gracilis)和蚕豆(Vicia faba L.)，对饮用水源水中不同粒径颗粒物的毒性效应进行了测试。结果表明，传统水处理工艺对小颗粒物(粒径<1 μm)的去除效果并不理想；经传统水处理工艺处理后，出水仍显著抑制发光菌发光强度以及纤细裸藻生长，对蚕豆的污染指数较高，可能存在一定健康隐患；进水中颗粒物粒径<1 μm组对发光菌发光强度和纤细裸藻生长的抑制效应显著大于粒径≥1 μm颗粒物组，蚕豆根尖的污染指数也更高，毒性更强。本研究结果可为饮用水的生物毒性检测和水处理工艺改良提供参考。
关键词: 颗粒物/
纤细裸藻/
蚕豆/
发光菌/
饮用水

Abstract:Substantial numbers of small particles (particle size <1 μm) are widely present in the fresh water environments and may adsorb and aggregate the micro-pollutants from the water to form new composite pollutants, thus affecting the biological, chemical and physical states of the aquatic ecosystem. Studies have shown that the smaller the particle size is, the more difficult it is to remove the particulate matter through the traditional water treatment process. Meanwhile, the pollutants adsorbed on the surface of particles will also remain in the water, which may affect the safety of drinking water. In this study, the particulate matter in the water at inlet and outlet of drinking water treatment plant in Nanjing was measured and compared. Moreover, three different types of model organisms (bacteria Photobacterium phosphoreum, protist Euglena gracilis and plant Vicia faba L.) were applied to test the toxicological effects of the particulate matter in drinking water. Results showed that the traditional water treatment process had no significant effect on the removal of small particles (particle size <1 μm). After traditional water treatment process, the outlet water still presents potential health risk, since it significantly inhibited the luminescence intensity of P. phosphoreum and the growth of E. gracilis, while the pollution index of V. faba root tips was relatively high. The toxicity of water was correlated with the size of particles present in the water. Stronger inhibition on the luminescence intensity of P. phosphoreum and on the growth of E. gracilis accompanied with increased pollution index of the V. faba root tips was observed when particles in the inlet water were smaller. Our results provide a scientific basis for monitoring the toxicity of drinking water and improving drinking water treatment processes.
Key words:particles/
Euglena gracilis/
Vicia faba L./
Photobacterium phosphoreum/
drinking water.

朱齐齐,赵鹏,张宏伟,等.天然水体中颗粒物吸附抗生素特征分析[J].环境科学学报, 2014, 34(5):1150-1156Zhu Q Q, Zhao P, Zhang H W, et al. Adsorption of antibiotics on the surface of aquatic particles[J]. Acta Scientiae Circumstantiae, 2014, 34(5):1150-1156(in Chinese)

杨艳玲,李星,李圭白.水中颗粒物的检测及应用[M].北京:化学工业出版社, 2007:2-190

Xiao Y, Zhao P, Yang Y, et al. Ecotoxicity evaluation of natural suspended particles using the microalga, Euglena gracilis[J]. Chemosphere, 2018, 206:802-808

张哲.饮用水源中不同粒径颗粒物吸附抗生素特征研究[D].天津:天津大学, 2014:2-135 Zhang Z. Characteristics of natural waters with different diameter particles adsorption of antibiotics[D]. Tianjin:Tianjin University, 2014:2-135(in Chinese)

汤鸿霄.环境水质学的进展--颗粒物与表面络合(上)[J].环境科学进展, 1993, 1(1):25-41Tang H X. Advances in environmental aquatic quality science-Particulates and surface complexation, Ⅰ[J]. Progress in Environmental Science, 1993, 1(1):25-41(in Chinese)

王占生,刘文君.微污染水源饮用水处理[M].北京:中国建筑工业出版社, 1999:3-305

戴婕,伍海辉,窦茵,等.颗粒计数仪器在给水处理工艺中的应用探索[J].给水排水, 2007, 33(9):27-30Dai J, Wu H H, Dou Y, et al. On application of particle counter in drinking water purification process[J]. Water & Wastewater Engineering, 2007, 33(9):27-30(in Chinese)

林涛,王磊磊,陈卫,等.饮用水处理中颗粒物数量变化及粒径分布规律[J].河海大学学报:自然科学版, 2008, 36(3):326-329Lin T, Wang L L, Chen W, et al. Amount and size distribution of particles in drinking water treatment[J]. Journal of Hohai University:Natural Sciences, 2008, 36(3):326-329(in Chinese)

Bilotta G S, Brazier R E. Understanding the influence of suspended solids on water quality and aquatic biota[J]. Water Research, 2008, 42(12):2849-2861

Wilber D H, Clarke D G. Biological effects of suspended sediments:A review of suspended sediment impacts on fish and shellfish with relation to dredging activities in estuaries[J]. North American Journal of Fisheries Management, 2001, 21(4):855-875

Kjelland M E, Woodley C M, Swannack T M, et al. A review of the potential effects of suspended sediment on fishes:Potential dredging-related physiological, behavioral, and transgenerational implications[J]. Environment Systems and Decisions, 2015, 35(3):334-350

Zhang X, Guo P, Huang J, et al. Effects of suspended common-scale and nanoscale particles on the survival, growth and reproduction of Daphnia magna[J]. Chemosphere, 2013, 93(10):2644-2649

Tse C Y, Chan K M, Wong C K. DNA damage as a biomarker for assessing the effects of suspended solids on the orange-spotted grouper, Epinephelus coioides[J]. Fish Physiology & Biochemistry, 2010, 36(2):141-146

徐丽丽,张哲,赵鹏,等.四种典型抗生素在饮用水源中不同粒径颗粒上的竞争吸附研究[J].环境科学学报, 2015, 35(12):3855-3861Xu L L, Zhang Z, Zhao P, et al. Competitive adsorption of four typical antibiotics on the different diameter particles in the drinking water resource[J]. Acta Scientiae Circumstantiae, 2015, 35(12):3855-3861(in Chinese)

常叶倩,于文汐,俞爽,等.氯代阻燃剂得克隆对纤细裸藻的生态毒性效应[J].生态毒理学报, 2017, 12(3):366-372Chang Y Q, Yu W X, Yu S, et al. Ecological toxic effects of a chlorinated flame retardant-Dechlorane Plus on Euglena gracilis[J]. Asian Journal of Ecotoxicology, 2017, 12(3):366-372(in Chinese)

刘羽,罗瑭秋琦,张亮,等. 4种酚类物质对蚕豆根尖细胞的遗传毒性研究[J].环境科技, 2019, 32(1):7-11Liu Y, Luo T Q Q, Zhang L, et al. Study on the genetic toxicity of four phenolic substances to Vicia faba root tip cells[J]. Environmental Science and Technology, 2019, 32(1):7-11(in Chinese)

魏东斌,赵慧敏,杜宇国.二苯甲酮类紫外防晒剂发光菌急性毒性及QSAR研究[J].生态毒理学报, 2017, 12(3):234-242Wei D B, Zhao H M, Du Y G, et al. Acute toxicity and QSAR studies on benzophenone-type UV-filters to Photobacterium[J]. Asian Journal of Ecotoxicology, 2017, 12(3):234-242(in Chinese)

国家环境保护局. GB/T 15441-1995水质急性毒性的测定发光细菌法[S].北京:中国标准出版社, 1996

Checcucci A, Colombetti G, Ferrara R, et al. Action spectra for photoaccumulation of green and colorless Euglena:Evidence for identification of receptor pigments[J]. Photochemistry and Photobiology, 1976, 23(1):51-54

Cheng P, Lee J W, Sichani H T, et al. Toxic effects of individual and combined effects of BTEX on Euglena gracilis[J]. Journal of Hazardous Materials, 2015, 284:10-18

刘炎,石小荣,崔益斌,等.高浓度氨氮胁迫对纤细裸藻的毒性效应[J].环境科学, 2013, 34(11):4386-4391Liu Y, Shi X R, Cui Y B, et al. Toxic effects of high concentrations of ammonia on Euglena gracilis[J]. Environmental Science, 2013, 34(11):4386-4391(in Chinese)

孔志明.环境毒理学[M].南京:南京大学出版社, 2008:297-301

刘春静,王蕾,焦永芳.动态光散射激光粒度仪的特点及其应用[J].现代科学仪器, 2011(6):160-163 Liu C J, Wang L, Jiao Y F. The features of dynamic laser light scattering particle size analyzer and its application[J]. Modern Scientific Instruments, 2011(6):160-163(in Chinese)

张哲,朱齐齐,赵鹏,等.饮用水源中不同粒径颗粒物吸附抗生素特征研究[J].环境科学学报, 2015, 35(7):2090-2098Zhang Z, Zhu Q Q, Zhao P, et al. Characteristics of antibiotics adsorption on particles with different diameters in natural waters[J]. Acta Scientiae Circumstantiae, 2015, 35(7):2090-2098(in Chinese)

刘顺亮,陶峰,宋晓红,等.南方某铀矿山废水对生物的急性毒性研究[J].生态毒理学报, 2018, 13(4):203-208Liu S L, Tao F, Song X H, et al. Study on the biological toxicity assessment of one southern uranium mine[J]. Asian Journal of Ecotoxicology, 2018, 13(4):203-208(in Chinese)

王娜.基于发光细菌生物毒性检测的混合毒性评价及预测研究[D].西安:西安建筑科技大学, 2014:3-122 Wang N. Study on evaluation and prediction for the combined toxicity of mixtures using luminescent bacteria test[D]. Xi'an:Xi'an University of Architecture and Technology, 2014:3-122(in Chinese)

Ma X Y, Wang X C, Ngo H H, et al. Bioassay based luminescent bacteria:Interferences, improvements, and applications[J]. Science of the Total Environment, 2013, 468-469:1-11

嵇志远,孟炯,黄允河.采用快速毒性测定仪测定水源水和自来水的综合毒性[J].净水技术, 2018, 37(9):73-77Ji Z Y, Meng J, Huang Y H. Determination of comprehensive toxicity of source water and drinking water with rapid toxtracer[J]. Water Purification Technology, 2018, 37(9):73-77(in Chinese)

刘红玲,杨本晓,于红霞,等.苯酚及其氯代物对大型溞的毒性效应和微观机理探讨[J].环境污染与防治, 2007, 29(1):33-36Liu H L, Yang B X, Yu H X, et al. Investigation of toxicity mechanisms of phenol & chlorophenols to Daphnia magna[J]. Environmental Pollution & Control, 2007, 29(1):33-36(in Chinese)

侯秀富.水体颗粒物对淡水藻类生长的影响及其反馈作用[D].厦门:华侨大学, 2013:3-133 Hou X F. Effects of water suspended particulate matter on the growth of freshwater algae and its feedback effect[D]. Xiamen:Huaqiao University, 2013:3-133(in Chinese)

Monarca S, Zani C, Richardson S D, et al. A new approach to evaluating the toxicity and genotoxicity of disinfected drinking water[J]. Water Research, 2004, 38(17):3809-3819

Degrassi F, Rizzoni M. Micronucleus test in Vicia faba root tips to detect utagen damage in fresh-water pollution[J]. Mutation Research/Environmental Mutagenesis and Related Subjects, 1982, 97(1):19-33

王超,阮鸿洁,豆捷雄,等.采用蚕豆根尖微核试验检测自来水厂水体的遗传毒性[J].癌变·畸变·突变, 2016, 28(6):481-483,490 Wang C, Yuan H J, Dou J X, et al. Use of the Vicia faba root tip micronucleus test to monitor the genotoxicity of tap water[J]. Carcinogenesis, Teratogenesis and Mutagenesis, 2016, 28(6):481-483, 490(in Chinese)

郭长城,喻国华,王国祥.河流泥沙对污染河水中污染物的吸附特性研究[J].生态环境, 2006, 15(6):1151-1155Guo C C, Yu G H, Wang G X. Adsorption properties of sediment to pollutants of contaminated river water[J]. Ecology and Environment, 2006, 15(6):1151-1155(in Chinese)

高鹏,莫测辉,李彦文,等.高岭土对喹诺酮类抗生素吸附特性的初步研究[J].环境科学, 2011, 32(6):1740-1744Gao P, Mo C H, Li Y W, et al. Preliminary study on the adsorption of quinolones to kaolin[J]. Environmental Science, 2011, 32(6):1740-1744(in Chinese)

任艳婷.水环境中不同粒径高岭土吸附喹诺酮类抗生素的特征分析[D].天津:天津大学, 2015:3-63 Ren Y T. Analysis of characteristics on the adsorption relationship between different size kaolin and quinolones in water[D]. Tianjin:Tianjin University, 2015:3-63(in Chinese)