孙晶,
欧阳少虎,
胡献刚,
周启星^,
南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津市城市生态环境修复与污染防治重点实验室, 天津 300071

作者简介: 孙晶(1990-),女,博士研究生,研究方向为生态毒理学,E-mail:sunjing90s@yeah.net.

通讯作者: 周启星,zhouqx@nankai.edu.cn

基金项目: 国家自然科学基金-山东联合基金（U1906222）；高等学校学科创新引智计划项目（T2017002）；国家自然科学基金面上项目（21677080）


中图分类号: X171.5

Effects of Three Carbonaceous Nanomaterials on the Developmental Toxicity, Oxidative Stress, and Metabolic Profile in Zebrafish

Sun Jing,
Ouyang Shaohu,
Hu Xiangang,
Zhou Qixing^,
Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China

Corresponding author: Zhou Qixing,zhouqx@nankai.edu.cn

CLC number: X171.5

-->

摘要
HTML全文
图(0)表(0)
参考文献(29)
相关文章
施引文献
资源附件(0)
访问统计

摘要:碳纳米材料（carbonaceous nanomaterials，CNMs）是人工纳米材料的重要组成部分，在各领域应用广泛。以斑马鱼为模式动物，比较了氧化石墨烯（graphene oxide，GO）、碳纳米管（carbon tube，CNT）和氧化石墨烯量子点（graphene oxide quantum dot，GOQD）3种典型CNMs对斑马鱼幼鱼的生长发育毒性，并探究了低浓度长时间暴露下3种CNMs对斑马鱼成鱼亚急性毒性效应及分子机制。结果表明，0.01～10 mg·L^-1的3种CNMs对斑马鱼胚胎发育无显著影响，但会诱导产生活性氧簇（ROS）和线粒体膜损伤，其毒性排序依次是GOQD > CNT > GO；环境相关浓度（0.01 mg·L^-1）下斑马鱼成鱼在3种CNMs中亚急性暴露21 d后，会引起斑马鱼腮和肾脏细胞衰老，同时抑制斑马鱼总超氧化物歧化酶（T-SOD）活性；代谢组学分析表明，3种CNMs对斑马鱼代谢组影响的顺序为GOQD > CNT > GO，T-SOD活性与代谢组学关联分析表明，脂肪酸和脯氨酸的变化是引起斑马鱼T-SOD活性变化的分子机理之一。该结果为评价3种典型CNMs对生态系统和人体健康的潜在影响提供了理论依据。
关键词: 氧化石墨烯/
碳纳米管/
量子点/
斑马鱼/
纳米毒性/
活性氧/
代谢组学

Abstract:As an important part of artificial nanomaterials, carbonaceous nanomaterials (CNMs) are widely applied in a plenty of areas such as energy, manufacturing and pharmaceutical industries. In the present study, the developmental toxicity, induced by three typical CNMs including graphene oxide (GO), carbon nanotube (CNT) and graphene oxide quantum dot (GOQD) was investigated in the typical model animal, zebrafish larva. The induced sub-acute toxicity at the low concentration of GO, CNT and GOQD was investigated in adult zebrafish, either. Moreover, the molecular mechanisms at the level of metabolomics were also explored. The results showed that there was a significant increase in reactive oxygen species (ROS), and mitochondrial membrane damage was caused by GO, CNT and GOQD in zebrafish larva. However, there was no significant developmental toxicity on zebrafish larva. The toxicity order in terms of the ROS increase and mitochondrial membrane damage was GOQD > CNT > GO. The chronic exposure at the typical environment-associated concentration (0.01 mg·L^-1) of CNMs can induce gill and kidney cell senescence of adult zebrafish. Meanwhile, it can also inhibit total superoxide dismutase (T-SOD) activity in adult zebrafish in the subacute toxicity test (21 d) at the concentration of 0.01 mg·L^-1. The metabolomics research revealed that the toxicity order at the environment-associated concentration acting on adult zebrafish was GOQD > CNT > GO; and it showed that fatty acids and proline turbulence may be responsible for one of the molecular mechanisms of T-SOD inhibition in adult zebrafish. This work can supply rationale to evaluate the potential risk of ecosystems and human health induced by the three typical CNMs.
Key words:graphene oxide/
carbon nanotube/
graphene oxide quantum dot/
zebrafish/
nanotoxicology/
reactive oxygen species/
metabolomics.

Lee J, Mahendra S, Alvarez P J J. Nanomaterials in the construction industry:A review of their applications and environmental health and safety considerations[J]. ACS Nano, 2010, 4(7):3580-3590

Lightcap I V, Kosel T H, Kamat P V. Anchoring semiconductor and metal nanoparticles on a two-dimensional catalyst mat. Storing and shuttling electrons with reduced graphene oxide[J]. Nano Letters, 2010, 10(2):577-583

Yang K J, Chen B L, Zhu X Y, et al. Aggregation, adsorption, and morphological transformation of graphene oxide in aqueous solutions containing different metal cations[J]. Environmental Science & Technology, 2016, 50(20):11066-11075

Zhu J X, Zhu T, Zhou X Z, et al. Facile synthesis of metal oxide/reduced graphene oxide hybrids with high lithium storage capacity and stable cyclability[J]. Nanoscale, 2011, 3(3):1084-1089

Choi W, Lahiri I, Seelaboyina R, et al. Synthesis of graphene and its applications:A review[J]. Critical Reviews in Solid State and Materials Sciences, 2010, 35(1):52-71

Lee Y A, Durandin A, Dedon P C, et al. Oxidation of guanine in G, GG, and GGG sequence contexts by aromatic pyrenyl radical cations and carbonate radical anions:Relationship between kinetics and distribution of alkali-labile lesions[J]. Journal of Physical Chemistry B, 2008, 112(6):1834-1844

Goodwin D G, Adeleye A S, Sung L, et al. Detection and quantification of graphene-family nanomaterials in the environment[J]. Environmental Science & Technology, 2018, 52(8):4491-4513

Jia P P, Sun T, Junaid M, et al. Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo[J]. Environmental Pollution, 2019, 247:595-606

Souza J P, Baretta J F, Santos F, et al. Toxicological effects of graphene oxide on adult zebrafish (Danio rerio)[J]. Aquatic Toxicology, 2017, 186:11-18

Hu X, Ouyang S H, Mu L, et al. Effects of graphene oxide and oxidized carbon nanotubes on the cellular division, microstructure, uptake, oxidative stress, and metabolic profiles[J]. Environmental Science & Technology, 2015, 49(18):10825-10833

Akhavan O, Ghaderi E. Toxicity of graphene and graphene oxide nanowalls against bacteria[J]. ACS Nano, 2010, 4(10):5731-5736

Ouyang S H, Li K W, Zhou Q X, et al. Widely distributed nanocolloids in water regulate the fate and risk of graphene oxide[J]. Water Research, 2019, 165:114987

Sun J, Zhou Q X, Hu X G. Integrating multi-omics and regular analyses identifies the molecular responses of zebrafish brains to graphene oxide:Perspectives in environmental criteria[J]. Ecotoxicology and Environmental Safety, 2019, 180:269-279

Garcia G R, Noyes P D, Tanguay R L. Advancements in zebrafish applications for 21st century toxicology[J]. Pharmacology & Therapeutics, 2016, 161:11-21

Song Y Y, Li R J, Zhang Y H, et al. Mass spectrometry-based metabolomics reveals the mechanism of ambient fine particulate matter and its components on energy metabolic reprogramming in BEAS-2B cells[J]. Science of the Total Environment, 2019, 651:3139-3150

Xu Y Y, Wang W J, Zhou J, et al. Metabolomics analysis of a mouse model for chronic exposure to ambient PM2.5[J]. Environmental Pollution, 2019, 247:953-963

Zhang X L, Zhou Q X, Zou W, et al. Molecular mechanisms of developmental toxicity induced by graphene oxide at predicted environmental concentrations[J]. Environmental Science & Technology, 2017, 51(14):7861-7871

Wang C, Yang X, Zheng Q, et al. Halobenzoquinone-induced developmental toxicity, oxidative stress, and apoptosis in zebrafish embryos[J]. Environmental Science & Technology, 2018, 52(18):10590-10598

Chen Y M, Hu X G, Sun J, et al. Specific nanotoxicity of graphene oxide during zebrafish embryogenesis[J]. Nanotoxicology, 2016, 10(1):42-52

Huang Z Y, Xu B, Huang X M, et al. Metabolomics reveals the role of acetyl-l-carnitine metabolism in gamma-Fe2O3 NP-induced embryonic development toxicity via mitochondria damage[J]. Nanotoxicology, 2019, 13(2):204-220

Zhao X S, Wang S T, Wu Y, et al. Acute ZnO nanoparticles exposure induces developmental toxicity, oxidative stress and DNA damage in embryo-larval zebrafish[J]. Aquatic Toxicology, 2013, 136:49-59

Mu L, Gao Y, Hu X G. Characterization of biological secretions binding to graphene oxide in water and the specific toxicological mechanisms[J]. Environmental Science & Technology, 2016, 50(16):8530-8537

Nouara A, Wu Q L, Li Y X, et al. Carboxylic acid functionalization prevents the translocation of multi-walled carbon nanotubes at predicted environmentally relevant concentrations into targeted organs of nematode Caenorhabditis elegans[J]. Nanoscale, 2013, 5(13):6088-6096

Chowdhury I, Duch M C, Mansukhani N D, et al. Colloidal properties and stability of graphene oxide nanomaterials in the aquatic environment[J]. Environmental Science & Technology, 2013, 47(12):6288-6296

Geng Y Q, Guan J T, Xu X H, et al. Senescence-associated beta-galactosidase activity expression in aging hippocampal neurons[J]. Biochemical and Biophysical Research Communications, 2010, 396(4):866-869

Geiger B, Nguyen H M, Wenig S, et al. From by-product to valuable components:Efficient enzymatic conversion of lactose in whey using beta-galactosidase from Streptococcus thermophilus[J]. Biochemical Engineering Journal, 2016, 116:45-53

Wang J, Li Y J, Lu L, et al. Polystyrene microplastics cause tissue damages, sex-specific reproductive disruption and transgenerational effects in marine medaka (Oryzias melastigma)[J]. Environmental Pollution, 2019, 254:10

He B, Ebarasi L, Hultenby K, et al. Podocin-green fluorescence protein allows visualization and functional analysis of podocytes[J]. Journal of the American Society of Nephrology, 2011, 22(6):1019-1023

Kim S, Ryu D Y. Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues[J]. Journal of Applied Toxicology, 2013, 33(2):78-89