黄荣1,
席贻龙1,2,
项贤领1,2,,
1. 安徽师范大学生态与环境学院, 芜湖 241000;
2. 皖江流域退化生态系统的恢复与重建省部共建协同创新中心, 芜湖 241000
作者简介: 李猛(1995-),男,硕士,研究方向为纳米材料的生态毒理效应,E-mail:1259305578@qq.com.
通讯作者: 项贤领,xiangxianling@163.com
基金项目: 国家自然科学基金资助项目（31872208）；生物环境与生态安全安徽省高校省级重点实验室专项中图分类号: X171.5
Effects of nano-TiO2 Exposure on Population Dynamics of Brachionus calyciflorus
Li Meng1,Huang Rong1,
Xi Yilong1,2,
Xiang Xianling1,2,,
1. School of Ecology and Environment, Anhui Normal University, Wuhu 241000, China;
2. Center of Cooperative Innovation for Recovery and Reconstruction of Degraded Ecosystem in Wanjiang City Belt, Wuhu 241000, China
Corresponding author: Xiang Xianling,xiangxianling@163.com
CLC number: X171.5
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摘要:因具有特殊的结构和性能，纳米材料的环境生物安全和潜在风险已引起人们的广泛关注，而由纳米TiO2所引起的轮虫种群动态变化尚鲜有报道。以萼花臂尾轮虫（Brachionus calyciflorus）为受试生物，通过急性和慢性毒性实验，探究纳米TiO2暴露对萼花臂尾轮虫种群动态的影响。结果表明，萼花臂尾轮虫48 h半致死浓度（48 h-LC50）值为30.20 mg·L-1；当纳米TiO2浓度≥1 mg·L-1时，萼花臂尾轮虫的最大种群密度、平均种群密度、平均种群增长率、平均非混交卵数量以及总雌体生产量受到显著抑制，且轮虫达到最大种群密度的时间更短，说明纳米TiO2的介入降低了环境容纳量，抑制了萼花臂尾轮虫种群繁殖力。此外，在2.0 mg·L-1和2.5 mg·L-1纳米TiO2处理组中休眠卵产量较对照组显著提高，当浓度≥1.5 mg·L-1时，种群平均混交率也显著提高，说明纳米TiO2暴露对萼花臂尾轮虫的有性生殖具有显著影响，这可归结于纳米TiO2显著提高了单位体积内产休眠卵的混交雌体生产量。
关键词: 纳米TiO2/
萼花臂尾轮虫/
急性毒性/
慢性毒性/
种群动态
Abstract:Increasing attention has been paid to the challenging issue of environmental biosafety and potential risks of nanomaterials due to its special structure and property. Few of these efforts, however, have focused on the effects of nano-TiO2 on the population dynamic of rotifers. In this study, Brachionus calyciflorus was taken as the subject to conduct the acute and chronic toxicity experiment so as to explore the population dynamic of B. calyciflorus influenced by nano-TiO2 exposure. The results showed that the 48 h half lethal concentration (48 h-LC50) value of nano-TiO2 was 30.20 mg·L-1. In the chronic assay, when the concentration of nano-TiO2 was greater than or equal to 1 mg·L-1, the maximum population density, mean population density, mean population growth rate, mean number of amictic eggs and the total female production of B. calyciflorus were inhibited significantly. And it took a shorter time to reach the maximum population density. It is indicated that the intervention of nano-TiO2 could reduce the environmental capacity and inhibit the fecundity of B. calyciflorus. In addition, the production of resting eggs in nano-TiO2 treatments with concentration of 2.0 mg·L-1 and 2.5 mg·L-1 were significantly higher than that in the control group, and as the concentration of nano-TiO2 was higher than 1.5 mg·L-1, the mean mictic rate has also increased significantly. These results highlighted that nano-TiO2 had an obvious impact on the sexual reproduction of B. calyciflorus, which could be attributed to the fact that nano-TiO2 increased the production of mictic female with resting eggs per unit volume significantly.
Key words:nano-TiO2/
Brachionus calyciflorus/
acute toxicity/
chronic toxicity/
population dynamics.
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