吴红松
菏泽学院 生命科学系, 菏泽 274015

作者简介: 吴红松(1977-),女,讲师,研究方向为动物生态毒理学,E-mail:hellowhs@163.com.

基金项目: 山东省自然科学基金项目(ZR2010CM046)
山东省十二五动物生理生化与应用重点实验室项目(201106)


中图分类号: X171.5

Effect of Diethyl Phthalate on the Biochemical Markers of Kidney and Brain in Cyprinus carpio Linnaeus

Wu Hongsong
Department of Life Science, Heze University, Heze 274015, China

CLC number: X171.5

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摘要:为探讨邻苯二甲酸二乙基己酯(DEHP)对鱼类肾、脑组织生物标志物的影响，将鲤鱼分别在浓度为5、20、80、160 mg·L^-1的DEHP水体，暴露20 d，以水和吐温-80为对照，测定肾脑组织中多种酶活性和丙二醛(MDA)含量的变化。结果表明：与水对照组相比，吐温-80组所测各项指标，差异均不显著。与水和吐温-80对照组相比，各染毒组，肾脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和抗羟自由基、抗超氧阴离子的活力均显著降低(P<0.05或P<0.01)。谷胱甘肽过氧化物酶(GSH-PX)酶活性呈现先升高后降低，在5、20、80 mg·L^-1浓度组显著升高(P<0.05)。丙二醛(MDA)含量在80、160 mg·L^-1浓度下，显著升高(P<0.05),但脑组织中变化不显著。脑中一氧化氮合酶(NOS)在各浓度组显著升高(P＜0.05或P＜0.01)；乙酰胆碱酯酶(AChE)在80、160 mg·L^-1时降低显著(P<0.05)；钙调神经磷酸酶(CaN)呈先升高后降低的趋势，在20 mg·L^-1下，升高显著(P<0.01)，在80、160 mg·L^-1浓度组，降低极显著(P<0.01)；抗羟自由基、抗超氧阴离子活性均显著下降(P＜0.05或P＜0.01)。可见，在实验浓度范围内，DEHP对鲤鱼具有一定免疫毒性和神经毒性，进一步的机理有待研究。
关键词: 邻苯二甲酸二乙基己酯/
鲤鱼/
肾/
脑/
生物标志物/
免疫毒性/
神经毒性

Abstract:In order to investigate the effect of DEHP (di-(2-ethylhexyl) phthalate) on the biochemical markers of kidney and brain in Cyprinus carpio (C. carpio), a 20-day period of experiment was conducted at four experimental concentrations of 5, 20, 80 and 160 mg·L^-1. H₂O and Tween-80 were used as the controls respectively. The results showed that, when compared with the H₂O control, all indexes of the Tween-80 control showed no significantly difference. While compared with the two controls, with the increasing of DEHP, the activities of the superoxide dismutase (SOD), catalase (CAT), resisting superoxide anion (·O^2-) and scavenging hydroxyl free radical (·OH^-) were all decreased significantly (P<0.05 or P<0.01) in the kidney. The activities of the glutathione peroxidase (GSH-Px) was increased first and then decreased, especially in the groups 5, 20, 80 mg·L^-1 (P<0.05). The malonaldehyde (MDA) increased remarkably in the groups of 80,160 mg·L^-1 in the kidney, but it showed no significantly differences in the brain. What is more, in the brain, the activity of nitric oxide synthase (NOS) was increased significantly in all the experimental groups (P<0.05 or P<0.01). On the contrary, the acetylcholinesterase (AChE) activity was significantly inhibited in the groups including 80, 160 mg·L^-1 DEHP (P<0.05). With the increasing of DEHP, the calcineurin (CaN) activity was increased first and then decreased. It increased significantly in 20 mg·L^-1 (P<0.01) and decreased remarkably when the dose were 80, 160 mg·L^-1 (P<0.01). Both resisting superoxide anion (·O^2-) and scavenging hydroxyl free radical (·OH^-) were decreased remarkably (P<0.05 or P<0.01). These results suggested that DEHP imposes immune toxicity and neurotoxicity to carp in the range of the experimental concentrations. The influence mechanism will need to be researched.
Key words:di-(2-ethylhexyl) phthalate/
Cyprinus carpio Linnaeus/
kidney/
brain/
biochemical marker/
immunotoxicity/
neurotoxicity.