陈晔¹,
陈荣^1,,,
莫正平¹
厦门大学环境与生态学院, 近海海洋环境科学国家重点实验室, 厦门 361102

作者简介: 陈晔(1991-),女,硕士,研究方向为生态毒理学,E-mail:360321490@qq.com.

通讯作者: 陈荣,chenrong@xmu.edu.cn ;

基金项目: 海洋公益性项目"海洋污染生物效应快速监测与评价技术应用示范"子任务－海洋污染效应生物标志物技术集成与研发II (201005016)


中图分类号: X171.5

The Effects of Cd and Pb on the Levels of Hepatic HSPs mRNA in Sebastiscus marmoratus

Chen Ye¹,
Chen Rong^1,,,
Mo Zhengping¹
State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China

Corresponding author: Chen Rong,chenrong@xmu.edu.cn ;

CLC number: X171.5

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摘要:为了解重金属污染对海洋鱼类热休克蛋白(HSPs)基因表达的影响,将褐菖鲉(Sebastiscus marmoratus)分别暴露于1.6、8、40、200、500 μg·L^-1Cd、Pb溶液中,用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)定量检测褐菖鲉肝脏HSP60、HSP70、HSP90、HSC70 mRNA表达量。结果表明:Pb仅在40 μg·L^-1时显著抑制HSP60、HSP90、HSC70 mRNA表达量,8 μg·L^-1时即可显著抑制HSP70 mRNA表达量,并在40 μg·L^-1时达到最小值;Cd对HSP60、HSP90、HSC70的诱导不明显,但能显著诱导HSP70,并在500 μg·L^-1时达到最大值。相比之下,褐菖鲉肝HSP70基因对重金属Cd、Pb污染较为敏感,有潜力成为监测海洋重金属污染的预警分子。
关键词: Cd/
Pb/
褐菖鲉/
肝脏/
热休克蛋白/
HSPs/
环介导等温扩增

Abstract:In order to understand the effects of heavy metal pollution on the gene expression of heat shock proteins (HSPs) in marine fish, Sebastiscus marmoratus were exposed to different concentrations (i.e., 1.6, 8, 40, 200, 500 μg·L^-1) of Cd or Pb for 5 days. A quantitative loop-mediated isothermal amplification (LAMP) method was used to analyze the hepatic mRNA levels of HSP60, HSP70, HSP90, and HSC70. Under the Pb exposure, the mRNA levels of hepatic HSP60, HSP90, HSC70 were not significantly reduced except in the 40 μg·L^-1 treatment, whereas the mRNA levels of HSP70 were significantly decreased by 8 μg·L^-1 Pb, and reached the minimum when Pb was 40 μg·L^-1. Under the Cd exposure, the mRNA levels of hepatic HSP60, HSP90, HSC70 were not significantly affected;in contrast, mRNA levels of HSP70 were significantly increased and peaked at 500 μg·L^-1 Cd. In conclusion, the hepatic HSP70 was relatively more sensitive to heavy metal exposure than the other HSPs, and thus had the potential to be the early warning for heavy metal pollution in marine environment.
Key words:Cd/
Pb/
Sebastiscus marmoratus/
liver/
heat shock proteins/
HSPs/
loop-mediated isothermal amplification.