王海斌^1,3,
叶江华^2,3,
孔祥海¹,
丁力¹,
邱丰艳¹,
贾小丽^2,3,,
1. 龙岩学院 生命科学学院, 龙岩 364012;
2. 武夷学院 生态与资源工程学院, 武夷山 354300;
3. 福建农林大学 福建省农业生态过程与安全监控重点实验室, 福州 350002

作者简介: 王海斌(1983-),男,汉族,博士,讲师,研究方向为植物生理与分子生态学,E-mail:w13599084845@sina.com.

通讯作者: 贾小丽,jcjyx@126.com

基金项目: 南平市科技计划项目(N2013X01-6)；福建省农业生态过程与安全监控重点实验室(福建农林大学)开放基金资助；武夷学院青年专项(XQ201015)；龙岩学院“人才引进项目”(LB2015001)


中图分类号: X171.5

Comparative Study on Biological Responses and Cu Speciation in Tissues of Two Different Tea Trees after Cu Exposure

Wang Haibin^1,3,
Ye Jianghua^2,3,
Kong Xianghai¹,
Ding Li¹,
Qiu Fengyan¹,
Jia Xiaoli^2,3,,
1. College of Life Sciences, Longyan University, Longyan 364012, China;
2. College of Ecology and Resources Engineering, Wuyi University, Wuyishan 354300, China;
3. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou 35002, China

Corresponding author: Jia Xiaoli,jcjyx@126.com

CLC number: X171.5

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摘要:为了分析不同茶树对Cu胁迫的生理响应及其组织中不同化学形态Cu的累积特性,本研究采用水培法,探讨铁观音、肉桂2种茶树在不同浓度Cu胁迫下,茶树根系活力、叶绿素含量、茶树组织Cu含量及不同化学形态Cu含量的变化,以期为重金属对茶树毒害机理和茶树对重金属的自我防御研究提供理论依据。结果表明,随着Cu胁迫浓度的增加,茶树根系活力及叶片叶绿素含量呈现下降趋势,而与对照相比,铁观音下降的幅度远高于肉桂。不同茶树品种在相同浓度Cu胁迫下,其根、茎、叶组织的Cu含量差异不显著,而不同组织中则表现为根>叶>茎。不同化学形态Cu含量分析结果表明,随着Cu胁迫浓度的升高,2种茶树根部可交换态Cu、碳酸盐结合态Cu、有机结合态Cu含量百分比呈现下降趋势,而铁锰氧化态Cu和残留态Cu呈现上升趋势;茎部可交换态Cu、有机结合态Cu含量百分比呈现下降趋势,碳酸盐结合态Cu、铁锰氧化态Cu和残留态Cu呈现上升趋势;叶部可交换态Cu、碳酸盐结合态Cu、有机结合态Cu含量、铁锰氧化态Cu含量的百分比均呈现上升趋势,而残留态Cu呈现下降趋势。进一步分析发现,Cu胁迫下,铁观音茶树根、叶部主要以采取提高有机结合态Cu的形式来降低Cu离子毒害,而肉桂则以提高铁锰氧化态Cu形式降低Cu离子毒害,叶部则以提高铁锰氧化态Cu和残留态Cu的形式降低Cu离子毒害。可见,不同的茶树在Cu胁迫下所表现解毒模式存在着一定的差异。
关键词: 铜胁迫/
茶树/
生理特性/
化学形态

Abstract:In order to analyze physiological responses of different tea trees to Cu stress and accumulation characteristics of Cu with different chemical forms in different tissues of tea tree, this paper adopted water culture method, and investigated root activity, chlorophyll content, Cu content in different tissues and Cu content with different chemical forms of Tieguanyin and Rougui tea tree under Cu stress with different concentrations. It is hoping to provide theoretical foundation for studies on the mechanism of heavy metal toxicity in tea tree and self-defense of tea tree to heavy metal. Results showed that root activity and chlorophyll content of tea tree descreased with the increase of Cu concentration, but decrease of Tieguanyin was larger than Rougui as compared with the control. At the same concentration of Cu stress, Cu contents in tea tree root, shoot and leaf had no significant difference among different treatments, and followed the order as root> leaf> shoot. The content percentages of exchangeable Cu, carbonate-bound Cu, organic-bound Cu from two tea tree roots had downward trends, while Cu with Fe-Mn oxidation state and residual Cu had upward trends as stress concentration increased. Content percentages of exchangeable Cu, organic-bound Cu in the shoot decreased, and carbonate-bound Cu, Cu with Fe-Mn oxidation state, and residual Cu increased. Content percentages of exchangeable Cu, carbonate-bound Cu, organic-bound Cu and Cu with Fe-Mn oxidation state in the leaf appeared to increase, while residual Cu declined. Further analysis indicated that organic-bound Cu was mainly used to reduce Cu²⁺ toxicity under Cu stress in roots and leaves of Tieguanyin. In contrast, Rougui roots reduced Cu²⁺ toxicity by increasing Cu with Fe-Mn oxidation state and its leaves reduced Cu²⁺ toxicity by increasing Cu with Fe-Mn oxidation state and residual Cu. Thus it is indicated that detoxification patterns of different tea tree varieties are different under Cu stress.
Key words:Cu stress/
tea tree/
physiological characteristics/
chemical forms.