郑莉娇¹,
杜青平^1,,,
刘建新¹,
王柯英¹,
许燕滨¹,
熊开容¹,
石瑛²
1. 广东工业大学 环境科学与工程学院, 广州 510006;
2. 太原师范学院 生物系, 太原 030031

作者简介: 郑莉娇(1990-),女,硕士研究生,研究方向为环境毒理学,E-mail:m18819462798@163.com.

通讯作者: 杜青平,qpdu2008@126.com ;

基金项目: 国际科技合作子项目(2013DFG50150)；广东省科技计划项目(2015A3FC0023，2013B020600007)；广东省-教育部产学研结合重大项目(2013B090200016)；山西省高等学校教学改革项目(J2014092)


中图分类号: X171.5

Combined Toxic Effects of Nano Titanium Dioxide (Nano-TiO₂) and 1,2,4-Trichlorobenzene (1,2,4-TCB) on Gill Tissues and Chromosome of Zebrafish

Zheng Lijiao¹,
Du Qingping^1,,,
Liu Jianxin¹,
Wang Keying¹,
Xu Yanbin¹,
Xiong Kairong¹,
Shi Ying²
1. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China;
2. Department of Biology, Taiyuan Normal University, Taiyuan 030031, China

Corresponding author: Du Qingping,qpdu2008@126.com ;

CLC number: X171.5

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摘要:采用组织病理切片法、微核及彗星试验研究nano-TiO₂和1,2,4-TCB联合染毒对斑马鱼鳃组织结构、细胞微核率及DNA损伤的毒性效应。结果表明，单独nano-TiO₂暴露不会导致斑马鱼鳃组织结构损伤，也不会引起明显的微核及染色体损伤。1,2,4-TCB(≥10 mg·L^-1)会引起鳃组织和细胞染色体的损伤。nano-TiO₂与1,2,4-TCB联合作用会加重斑马鱼的鳃组织结构损伤，出现鳃小片排列严重紊乱，表皮细胞断失严重，鳃小片上皮细胞坏死脱落，鳃丝结构模糊等症状。两者联合作用会引起鳃细胞微核率增加和彗星细胞率的增加，呈现一定的剂量-效应关系，且两者间的联合作用具有协同作用。对彗星试验的尾部DNA百分比、尾长及尾距的分析发现，与对照组和单独作用相比，两者联合作用对彗星指标均有显著增强作用，这表明两者联合作用对染色体的损伤属于非特定片段损伤。
关键词: nano-TiO₂/
1,2,4-TCB/
斑马鱼/
联合毒性/
DNA损伤/
微核/
鱼鳃

Abstract:Combined toxicity of nano-TiO₂ and 1,2,4-trichlorobenzene (1,2,4-TCB) on zebrafish gills was studied by pathological observation test, micronucleus test and comet assay, using zebrafish and its gill tissues as experimental objects. The results showed that nano-TiO₂ did not cause significant structure damage in gill tissues, micronucleus and other chromosome damage. But gill tissues and chromosomes were damaged by 1,2,4-TCB (≥10 mg·L^-1). In the combined groups of 1,2,4-TCB and nano-TiO₂, the structure damages of gill tissues were aggravated. Serious disorder of the gill lamellae, broken epidermal cells, necrosis of gill epithelial cells and fuzzy structure of gill filaments were observed. The micronucleus percentages and the percentages of comet cells of gill cells were increased with increasing concentrations of nano-TiO₂ and 1,2,4-TCB in a dose-effect manner, and the damages caused by nano-TiO₂ and 1,2,4-TCB combined treatment showed synergetic effects. The comet indicators of tail DNA percentage, tail length, tail moment of gill cells were all increased significantly in groups treated by mixture of nano-TiO₂ and 1,2,4-TCB compared to the control groups, which suggested that the damages of chromosomes of gill cells were not particular piece damage of chromosomes.
Key words:nano-TiO₂/
1,2,4-TCB/
zebrafish/
combined toxicity/
DNA damage/
micronucleus/
gill cell.