- 黄昆仑导师简介 -
基本信息
目前指导研究生
在籍
- 博士研究生6人 (其中 2012级:1 2013级:2 2014级:2 2015级:1) 硕士研究生7人 (其中 2013级:1 2014级:4 2015级:2)
不在籍
- 博士研究生9人 (其中 2008级:2 2009级:2 2010级:2 2011级:2 2012级:1) 硕士研究生33人 (其中 2005级:4 2006级:3 2007级:4 2008级:4 2009级:4 2010级:6 2011级:3 2012级:3 2013级:2)
个人简历
联系方式
电话: **
传真: **
E-mail: hkl009@163.com
通信地址:中国农业大学东校区302信箱 邮编:100083
学习工作经历
1990—1997年,新疆农业科学院,从事优质高产水稻育种工作、农药残留研究;
1997—2000年,新疆农业大学,在读硕士,主要研究水果采后病理;
2000—2003年,中国农业大学,在读博士,主要研究转基因产品食用安全和转基因成分检测技术;
2003至今,中国农业大学,从事转基因产品食用安全评价与检测、食品安全风险评估技术与检测技术。此外先后出访美国、加拿大、日本、欧盟、泰国等国家和地区。先后指导培养硕士、博士研究生20余名。
现任职务(社会兼职等)
国家农业转基因生物安全委员会委员
全国农业转基因生物技术标准委员会委员
农业部农产品质量监督检验测试中心(北京)常务副主任
农业部农业转基因生物食用安全监督检验测试中心(北京)常务副主任。
国际食品法典委员会中国联络处农业组生物技术食品专家委员会委员。
奖励及其他
国家科技进步奖二等奖一项
教育部科技进步2等奖一项
北京市科技进步三等奖一项
中国农业大学首届“师恩杯”“我最喜欢的导师”
教育部成果鉴定6项
遴选学科专业
不公开
论文与专著
代表著作与论文
已发表论文200余篇,其中SCI收录100余篇,发表杂志涉及:《Journal of Agriculture and Food Chemistry》、《Journal of the Science of Food and Agriculture》、《Food control》、《Food Science》、《Plant Cell Repots》、《Biotechnol Lett》、《LWT-Food Science and Technology》、《European Food Research and Technology》、《Food Chemistry Toxicology》、《Carbohydrate Research》、《Chinese Journal of Agricultural biotechnology》等,申请专利26项,获得授权国家专利4项。主编和参编教材8部包括《转基因食品安全评价与检测技术》(主编)、《转基因食品社会文化伦理透视》(主编)、《国际转基因生物食用安全检测及其标准化》(主编)、《食品营养与安全》(主编)、《转基因食品》(主编)、《食品安全学》(参编)、《食品生物技术概论》(参编)等。
代表性SCI论文:
1. Safety assessment of genetically modified rice expressing human serum albumin from urine metabonomics and fecal bacterial profile. Food Chem. Toxicol. .2014, In press,X. Qi, S. Chen, Y. Sheng, M. Guo, Y. Liu, X. He, K. Huang, W. Xu
2. A 90-day subchronic feeding study of genetically modified rice expressing Cry1Ab protein in Sprague–Dawley rats,Transgenic Research , 2014, DOI 10.1007/s11248-014-9844-6. H. Song, X.He, S. Zou, T. Zhang, Y. Luo, K. Huang, Z. Zhu, W Xu
3. Toxicological evaluation of lactase derived from recombinant Pichia pastoris. Plos One. 2014 9(9): e106470.S. Zou, X. Hea, Y. Liu, D. Chen, Y. Luo, K. Huang, W. Zhang, W. Xu
4. A-T linker adapter polymerase chain reaction for determining flanking sequences by rescuing inverse PCR or thermal asymmetric interlaced PCR products. Analytical biochemistry. 2014,466C:24-26. Q. Trinh; P. Zhu; H. Shi; W. Xu; J. Hao; Y. Luo; K. Huang
5. Combination of metagenomics and culture based methods to study the interaction between ochratoxin A and gut microbiota. Toxicological Sciences. 2014, 141(1):314-323. M. Guo, K. Huang, S.Chen, X. Qi, X. He, W.H. Cheng, Y. Luo, K. Xia, W. Xu
6. Subchronic toxicity study in vivo and allergenicity study in vitro for genetically modified rice that expresses pharmaceutical protein (human serum albumin). Food Chem. Toxicol. 2014,72:242-246 Y. Sheng, X. Qi, Y. Liu, M. Guo, S. Chen, X. He, K. Huang, W. Xu
7Ochratoxin A induced early hepatotoxicity: new mechanistic insights from microRNA, mRNA and proteomic profiling studies.Scientific reports. 2014,4: 5163. X.Z Qi, X. Yang, S.Y. Chen, X.Y. He, H. Dweep, M.Z. Guo, W.H. Cheng, W.T. Xu, Y.B. Luo, N. Gretz, Q. Dai, K.L. Huang
8Protective effect of N-acetylcysteine against DNA damage and S-phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK-293). Food Chem. Toxicol. 2014,70:40-47. Q. Yang, L. Shi, K.L. Huang, W.T. Xu*
9Development and Application of a Quantitative Loop mediated Isothermal Amplification Method for Detecting Genetically Modified Maize MON863. J. Sci. Food Agric. 2014,DOI: 10.1002/jsfa.6707. S.C. Huang, Y.C Xu, X.H. Yan, Y. Shang, P.Y. Zhu , W.Y. Tian , W.T. Xu.
10Central role of Nix in the autophagic response to orchratoxin AFood Chem. Toxicol. 2014,69:202-209.X.L. Shen, B.Y. Zhang, R. Liang, W.H. Cheng, W.T. Xu, Y.B. Luo, C.H. Zhao, K.L. Huang.
11Analysis of Individual and Combined Effects of Ochratoxin A and Zearalenone on HepG2 and KK-1 Cells with Mathematical Models. Toxins. 2014,6(4):1177-92. Y.Z. Li, B.Y. Zhang, X.Y. He, W.H. Cheng, W.T. Xu, Y.B. Luo, R. Liang , H.S. Luo, K.L. Huang.
12 Protective role of the mitochondrial Lon protease 1 in ochratoxin A-induced cytotoxicity in HEK293 cells. Journal of Proteomics. 2014,101:154-68.B.Y. Zhang, X.L. Shen, R. Liang, Y.Z. Li, K.L. Huang, C.H. Zhao, Y.B. Luo, W.T. Xu.
13Restriction enzyme cutting site distribution regularity for DNA looping technology.Gene. 2014,534(2):222-228.Y. Shang, N. Zhang, P.Y. Zhu, Y.B. Luo, K.L. Huang, W.Y. Tian, W.T. Xu.
14A peach (Prunuspersica)-speci?c gene, Lhcb2, used as an endogenous reference gene for qualitative and real-time quantitative PCR to detect fruit products. LWT-Food Science and Technology. 2014,55(1):218-223Y Shang, P Zhu, K Huang, W Liu, W Tian, Y Luo, W.T. Xu.
15Randomly broken fragment PCR with 5’ end-directed adaptor for genome walking. Scientific reports. 2013,3: 3465.W.T. Xu, Y. Shang, P.Y. Zhu, Z.F. Zhai, J.He, K.L. Huang, Y.L. Luo.
16An iTRAQ-based mitoproteomics approach for profiling the nephrotoxicity mechanisms of ochratoxin A in HEK 293 cells. Journal of Proteomics. 2013,78(14):398-415. X.L. Shen, Y. Zhang, W.T. Xu, R. Liang, J.J. Zheng, Y.B. Luo, Y. Wang, K.L. Huang
17Zinc protects HepG2 cells against the oxidative damage and DNA damage induced by ochratoxin A. Toxicol Appl Pharm. 2013,268(2):123-131. J Zheng, Y Zhang, W.T. Xu, YB Luo, J Hao, X Shen, X Yang, X Li, K Huang.
18Comparative proteomics and physiological characterization of Arabidopsis thaliana seedlings in responses to Ochratoxin A.Plant molecular biology.2013,82(4-5):321-337.Y Wang, J Hao, W Zhao, Z Yang, W Wu, Y Zhang, W.T. Xu*, YB Luo, K Huang.
19A 90-day feeding study of glyphosate-tolerant maize with the G2-aroA gene in Sprague–Dawley rats. Food Chem. Toxicol.2013,51: 280-287. Y Zhu, X He, Y, S Zou,X Zhou, KHuang, W Xu.
20Effects of genetically modified T2A-1 rice on the GI health of rats after 90-day supplement. Scientific reports. 2013,3:1962.Y Yuan, W Xu, X He, H Liu, S Cao, X Qi, K Huang, Y Luo.
21The transcriptional response of virulence genes in Listeria monocytogenes during inactivation by nisin. Food Control. 2013,31(2):519–524.H Shi,Q Trinh, W Xu, Y Luo, W Tian, K Huang.
22Development and optimization of an efficient method to detect the authenticity of edible oils. Food Control. 2013,31(1):71-79.J He, W Xu, Y Shang, P Zhu, X Mei, W Tian, K Huang.
23Single universal primer multiplex ligation-dependent probe amplification with sequencing gel electrophoresis analysis.Analytical Biochemistry. 2013,443(2):243-248.Y Shang, P Zhu, W Xu, T Guo, W Tian, Y Luo, K Huang.
24Restriction enzyme cutting site distribution regularity for DNA looping technology. Gene. 2013,534(2):222-8.Y Shang, N Zhang, P Zhu, Y Luo, K Huang, W Tian, W Xu
25Transcript and protein profiling analysis of OTA-induced cell death reveals the regulation of the toxicity response process in Arabidopsis thaliana. Journal of Experimental Botany. 2012,63(5):2171-2087. Y Wang, X Peng, W Xu, Y Luo, W Zhao, J Hao, Z Liang, Y Zhang, K Huang.
26 A novel Universal Primer-Multiplex-PCR method with sequencing gel electrophoresis analysis. PLOS ONE. 2012,7(1): e22900. W Xu, Z Zhai, K Huang, N Zhang, Y Yuan, Y Shang, Y Luo.
27A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast. PLOS ONE. 2012,7(3): e32943.S Cao, W Xu, N Zhang, Y Wang, Y Luo, X He, K Huang.
28Safety assessment of transgenic Bacillus thuringiensis rice T1c-19 in Sprague–Dawley rats from metabonomics and bacterial profile perspectives.IUBMB Life. 2012, 64(3):242-250. S Cao, X He, W Xu, Y Luo,Y Yuan, P Li, B Cao, H Shi, K Huang
29The effect of genetically modified Lactobacillus plantarum 590 on the gut health of Sprague–Dawley rats. IUBMB Life. 2012,64(7):617–627.H Liu, W Xu, Y Yuan, S Cao, X He, S Li, K Huang, Y Luo.
30 Loop-linker PCR: An advanced PCR technique for genome walking. IUBMB Life. 2012,64(10):841–845. Q Trinh, H Shi, W Xu, J Hao, Y Luo, K Huang.
31A universal primer multiplex PCR method for typing of toxinogenic Pseudomonas aeruginosa. Appl. Microbiol. Biot. 2012,95(6):1579-87. H Shi, Q Trinh, W Xu, B Zhai, Y Luo, K Huang.
32Subchronic feeding study of stacked trait genetically-modified soybean (3?5423×40-3-2) in Sprague-Dawley rats. Food Chem. Toxicol.2012,50(9): 3256-3263. X Qi, X He, Y Luo, S Li, S Zou, S Cao, M Tang, B Delaney, W Xu, K Huang.
33Safety assessment of dehydration-responsive element-binding (DREB) 4 protein expressed in E. coli. Food Chem. Toxicol. 2012,50(11): 4077-4084. B Cao, X He, Y Luo, L Ma, P Liu, S Cao, X Liu,S Zou, W Xu,K Huang
34A 90-day subchronic feeding study of genetically modified maize expressing Cry1Ac-M protein in Sprague–Dawley rats. Food Chem. Toxicol.2012,50(9): 3215–3221.P Liu, X He, D Chen, Y Luo, S Cao, H Song, T Liu, K Huang, W XU.
35An A-T Linker Adapter PCR Method for Chromosome Walking without Restriction-site Cloning Bias. Analytical Biochemistry. 2012,425: 62–67.Q Trinh, W Xu , H Shi, Y Luo, K Huang.
36Establishment of a viable cell detection system for microorganisms in wine based on ethidiummonoazide and quantitative PCR. Food Control. 2012, 27(1): 81-86. H Shi, W Xu, Q Trinh, Y Luo, Z Liang, Y Li, K Huang.
37Potential allergenicity research of Cry1C protein from genetically modified rice.Regul Toxicol Pharm. 2012,63: 181–187. S Cao; X He; W Xu, Y Luo; W Ran; L Liang; Y Dai; K Huang.
38Comparative analysis of the proteomic and nutritional composition of transgenic rice seeds with Cry1ab/ac genes and their non-transgenic counterparts. Journal of cereal science. 2012,55(2):236-233. Y Wang, W Xu, W Zhao, J Hao, Y Luo, X Tang, Y Zhang, K Huang.
39Establishment and optimization of a wheat germ cell-free protein synthesis system and its application in venom kallikrein. Protein Expres. Purif. 2012,84(2):173-80. Y Wang, W Xu, X Kou, Y Luo, Y Zhang, B Ma, M Wang, K Huang.
40 Metabonomics Study of Transgenic Bacillus Thuringiensis Rice (T2A-1) Meal in a 90-Day Dietary Toxicity Study in Rats. Molecular BioSystems. 2011,7:2304 -2310. S Cao, W Xu, Y Luo, X He, Y Yuan, W Ran, L Liang, K Huang.
41The effect of various environmental factors on the ethidium monazite and quantitative PCR method to detect viable bacteria. J Appl. Microbiol. 2011,111, 1194–1204. H Shi., W Xu, Y Luo., L Chen, Z Liang, X Zhou, K Huang.
42Safety assessment of Cry1C protein from genetically modified rice according to the national standards of PR China for a new food resource. Regul Toxicol. Pharm. 2010,58,474–481. S Cao, X He, W Xu, W Ran, L Liang, Y Luo, Y Yuan, N Zhang, X Zhou, K Huang.
43Event-specific Detection of Stacked Genetically Modified Maize Bt11×GA21 by UP-M-PCR and Real-time PCR. J. Agric. Food Chem. 2009,57: 395–402. W Xu, Y Yuan, L Luo, W Bai, C Zhang, K Huang.
44 Safety Assessment of Cry1Ab/Ac fusion protein. Food Chem. Toxicol. 2009,47:1459–1465. W Xu, S Cao, X He, Y Luo, X Guo, Y Yuan, K Huang.
近五年承担的主要项目
主持国家级和省部级项目20余项:
国家重大专项“转基因生物新品种培育”重大项目“转基因生物的食用和饲用安全评价技术---任务八 玉米、小麦等转基因生物的食用安全评价”、“转基因生物食用安全评价与检测中心能力建设”(子课题主持);
农业部财政项目“转基因生物成分监测与突发事件应急处理的研究”(2014)、“转基因生物应急检测与科普宣传平台建设”(2013)、“蔬菜质量安全例行监测及普查”(2013)、“开展芹菜、韭菜、普通白菜等蔬菜用药基本情况专项调查”(2014)、“北京、天津地区蔬菜水果贮藏保鲜环节质量安全风险评估”(2013)等;
国家科技支撑计划“食品质量与安全产业技术研发与服务平台建设-粮油食品中主要污染物高通量分析技术研究”(子课题主持);
北京市科技计划项目“食品质量与安全产业技术研发与服务平台建设-粮油食品中主要污染物高通量分析技术研究”(子课题主持)。
农业部标准制订项目9项。
承担的教学工作
主讲课程有本科生课程《转基因食品现状与展望》、《转基因食品安全评价与检测技术》;研究生课程《转基因食品安全评价》、《分子生物学专题》、《科研诚信与学术规范》。