关键词:水稻; 抗性; 褐飞虱; 数量性状基因座; 遗传图谱 Analysis of QTLs for Brown Planthopper Resistance in Indica Rice WD15515 DENG Zhao**, SHI Shao-Jie**, WANG Hui-Ying, SHANG-GUAN Xin-Xin, LIU Bing-Fang, JING Sheng-Li, DU Bo, CHEN Rong-Zhi, ZHU Li-Li, HE Guang-Cun* State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China Fund:This study was supported by the National Natural Science Foundation of China (31230060) and the National Basic Key Research Program (973 Program) (2013CBA01403) AbstractBrown planthopper (BPH) is one of the most destructive insect pests of rice. WD15515 is an indica germplasm highly resistant to BPH. An F2 population was developed from the cross between 9311 and WD15515. One hundred F2 plants were genotyped by using SSR markers and evaluated for BPH-resistance by measuring the honeydew weight secreted by BPH (HW), the body weight increment (WB) and the body weight increment ratio (WRB). A total of four QTLs for BPH resistance were identified. The qBph2-1, based on HW (honeydew weight), was detected between RM71 and RM6911 on chromosome 2, with LOD score of 3.68 and explaining the 11.08% of phenotypic variation. The qBph2-2, based on both WB and WRB, was mapped between RM6911 and RM521 on chromosome 2, with LOD score of 3.31 and 4.05 and explaining 7.81% and 9.38% of the phenotypic variation, respectively. The qBph4, based on HW, WB and WRB, was detected between RM16996 and RM17075 on chromosome 4, with LOD score of 11.11, 13.81, and 15.41 and explaining 44.38%, 45.24%, and 52.40% of the phenotypic variation, respectively. The qBph9, based on HW, WB, and WRB, was detected on chromosome 9 between RM219 and RM6444, with LOD score of 2.59, 4.04, and 3.63. This locus explained 10.91%, 12.39%, and 10.01% of the phenotypic variation in this population, respectively. qBph4is a major gene for BPH-resistance. This result provides the new resources for BPH-resistance breeding.
图3 9311/WD15515 F2群体的RM16996基因型和抗虫性表型频率分布图Fig. 3 Frequency distributions of BPH-resistance phenotypes and genotypes in F2population derived from the cross of 9311/WD15515
表3 Table 3 表3(Table 3)
表3 水稻9311/WD15515 F2群体中抗褐飞虱QTL检测结果 Table 3 Brown planthopper resistance QTLs detected in 9311/WD15515 F2 population
QTL
性状 Trait
染色体 Chromosome
区间 Interval
LOD值 LOD score
贡献率 PVE (%)
加性效应 ADD
显性效应 DOM
Left marker
Right marker
qBph2-1
HW
2
RM71
RM6911
3.68
11.08
-0.85
-1.12
qBph2-2
WB
2
RM6911
RM521
3.31
7.81
-0.25
-0.12
WRB
4.05
9.38
-0.13
-0.05
qBph4
HW
4
RM16996
RM17075
11.11
44.38
-2.43
-1.45
WB
13.81
45.24
-0.72
0.01
WRB
15.41
52.40
-0.33
-0.18
qBph9
HW
9
RM219
RM6444
2.59
10.91
-0.55
1.54
WB
4.04
12.39
-0.05
0.50
WRB
3.63
10.01
-0.03
0.20
HW: honeydew weight; WB: weight of BPH; WRB: weight ratio of BPH. HW: 蜜露分泌量; WB: 虫体增重; WRB: 虫体增重比。
表3 水稻9311/WD15515 F2群体中抗褐飞虱QTL检测结果 Table 3 Brown planthopper resistance QTLs detected in 9311/WD15515 F2 population
图5 WD15515/9311//9311 BC1F1群体中抗褐飞虱表型和RM16996基因型频率分布图Fig. 5 Frequency distributions of BPH-resistance phenotypes and genotypes of RM16996 in the BC1F1 population derived from the cross of WD15515/9311//9311
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