苏荣欣^1,2,3, 杨仁俊^1,2, 齐崴^1,2,3, 何志敏^1,2
AuthorsHTML:苏荣欣^1,2,3, 杨仁俊^1,2, 齐崴^1,2,3, 何志敏^1,2
AuthorsListE:Su Rongxin^1,2,3, Yang Renjun^1,2, Qi Wei^1,2,3, He Zhimin^1,2
AuthorsHTMLE:Su Rongxin^1,2,3, Yang Renjun^1,2, Qi Wei^1,2,3, He Zhimin^1,2
Unit:1. 天津大学化工学院，天津 300072；2. 化学工程联合国家重点实验室(天津大学)，天津 300072；3. 天津化学化工协同创新中心，天津 300072
Unit_EngLish:1．School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2．State Key Laboratory of Chemical Engineering(Tianjin University), Tianjin 300072, China
3．Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300072, China
Abstract_Chinese:通过溶剂热法和改进的St?ber水解法制备了Fe₃O₄@SiO₂磁性纳米粒子, 并用透射电镜对纳米粒子进行了表征．采用聚多巴胺辅助法将β-葡萄糖苷酶固定在磁性纳米粒子上．具体考察了加酶量、多巴胺质量浓度和固定化时间等因素对酶活和酶固定率的影响规律, 获得了β-葡萄糖苷酶固定化的最适条件．结果表明, 当加酶量为50 U/g、多巴胺质量浓度为1.6 mg/mL、固定化为24 h时, 所制备的固定化β-葡萄糖苷酶活力为25.01 U/g, 明显优于传统戊二醛交联固定的β-葡萄糖苷酶活力．
Abstract_English:The Fe₃O₄@SiO₂ nanoparticles (NPs) were prepared through solvothermal and modified St?ber methods，and the morphology of the nanoparticles was characterized by TEM. β-glucosidase was immobilized on magnetic nanoparticles with the assistance of polydopamine. In this work，the effects of enzyme loadings，the mass concentration of dopamine and the immobilization time on enzyme activity and immobilization yield were systematically investigated. The results show that the immobilized β-glucosidase enzyme activity could reach 25.01 U/g when enzyme loading was 50 U/g，the mass concentration of dopamine was 1.6 mg/mL and the immobilization time was 24 h. It is superior to the enzyme activity of β-glucosidase immobilized by traditional glutaraldehyde crosslinking.
Keyword_Chinese:β-葡萄糖苷酶; 固定化; 磁性纳米粒子; 多巴胺
Keywords_English:β-glucosidase; immobilization; magnetic nanoparticle; dopamine

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