Jianyu Zhang (张建宇)——Associate Professor
Nationality:


Phone:


Email:
Jianyu.Zhang@tju.edu.cn

Office:
417-1, BLDG 24

School:
School of Pharmaceutical Science and Technology

ResearcherID:
I-3678-2016

Group weblink








Education Experience
2003-2008 Ph. D. Organic Chemistry Nankai Univ
1999-2003 Bachelor of Science Chemistry Nankai Univ

Professional Experience
2009-2014 Postdoc University of California, Berkeley
2014-2016 Asst Project Scientist University of California, Berkeley
2016- Associate Prof Tianjin Univ

Research Area
Our group research interest lies in investigating basic mechanism of bio-active molecular (including nitric oxide, hydride and methyl et al.) transfer reaction in enzyme or in solution, with a goal of 1) design of more efficient chemical catalysis as well as the designed enzyme; 2) rational design of corresponding inhibitor/drug basing on the mechanism exploration. This inspiring research area requires the combination the application of physical organic chemistry, biochemistry, chemical biology and molecular biology.
The most recently work refers to the understanding the role of compaction in methyl transfer reactions with the target of finding how the molecular motion in enzymes would affect the catalytic ability in the methyl transfer reaction. This study about the methyl transfer system had/will extended from catechol-O-methyltransferase (COMT) to glycine-N-methyltransferase (GNMT), Nicotinamide N-Methyltransferase (NNMT) and DNA\RNA demethylation with various experimental approaches such kinetic isotope effect, binding isotope effect, time-resolved spectrometers, hydrogen deuterium exchange with mass spectrometry (HDX-MS) as well as computational simulation.



Honors and Awards

Patents


Highlighted Publications
Jianyu Zhang and Judith P Klinman* “Convergent Mechanistic Features between the Structurally Diverse N- and O Methyltransferases: Glycine N Methyltransferase and Catechol O Methyltransferase” Journal of the American Chemical Society 2016, 138, 9158?9165.
Jianyu Zhang, Heather J. Kulik, Todd J. Martinez and Judith P Klinman* “Mediation of Donor-Acceptor Distance in An Enzymatic Methyl Transfer Reaction” Proceedings of the National Academy of Sciences of United States of America 2015, 112 (26), 7954-7959.
Jianyu Zhang and Judith P Klinman* “Enzymatic Methyl Transfer: Role of an Active Site Residue in Generating Active Site Compression that Correlates with Catalytic Efficiency” Journal of the American Chemical Society 2011, 133, 17134-17137.
Xiaoqing Zhu,* Jianyu Zhang, and Jinpei Cheng* “Mechanism and Driving Force of NO Transfer from S-Nitrosothiol to Cobalt(II) Porphyrin: a Detailed Thermodynamic and Kinetic Study”, Inorganic Chemistry 2007, 46, 592-600.
Xiaoqing Zhu,* Jianyu Zhang, and Jinpei Cheng* “Negative Kinetic Temperature Effect on the Hydride Transfer from NADH Analogue BNAH to the Radical Cation of N-Benzylphenothiazine in Acetonitrile” Journal of Organic Chemistry 2006, 71, 7007-7015.
Xiaoqing Zhu,* Jianyu Zhang, Lianrui Mei, and Jinpei Cheng* “Mechanism of NO Transfer from NO-Donors (SNAP and G-MNBS) to Ferrous Tetraphenylporphyrin in CH3OH” Organic Letters 2006, 8, 3065-3067.

ResearcherID Publications
Year Title Author(s) Source Volume
2018 Activity-Related Microsecond Dynamics Revealed by Temperature-Jump Forster Resonance Energy Transfer Measurements on Thermophilic Alcohol Dehydrogenase Vaughn, Morgan B.; Zhang, Jianyu; Spiro, Thomas G.; et al. Journal of the American Chemical Society 140
2016 CH···O Interactions Are Not the Cause of Trends in Reactivity and Secondary Kinetic Isotope Effects for Enzymatic SN2 Methyl Transfer Reactions Zhang, Jianyu; Klinman, Judith P. Biorxiv Preprint
2016 Convergent Mechanistic Features between the Structurally Diverse N- and O-Methyltransferases: Glycine N-Methyltransferase and Catechol O-Methyltransferase Zhang, J. Y.; Klinman, J. P. Journal of the American Chemical Society 138
2016 How Large Should the QM Region Be in QM/MM Calculations? The Case of Catechol O-Methyltransferase Kulik, Heather J.; Zhang, Jianyu; Klinman, Judith P.; et al. Journal of Physical Chemistry B 120
2015 High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-L-methionine Zhang, Jianyu; Klinman, Judith P. Analytical Biochemistry 476
2015 How large should the QM region be in QM/MM calculations? The case of catechol O-methyltransferase Kulik, Heather J; Zhang, Jianyu; Klinman, Judith P; et al. arXiv preprint
2015 Mediation of donor-acceptor distance in an enzymatic methyl transfer reaction Zhang, Jianyu; Kulik, Heather J.; Martinez, Todd J.; et al. Proceedings of the National Academy of Sciences of the United States of America 112
2011 Enzymatic Methyl Transfer: Role of an Active Site Residue in Generating Active Site Compaction That Correlates with Catalytic Efficiency Zhang, Jianyu; Klinman, Judith P. Journal of the American Chemical Society 133
2007 Mechanism and driving force of NO transfer from S-nitrosothiol to cobalt(II) porphyrin: A detailed thermodynamic and kinetic study Zhu, Xiao-Qing; Zhang, Jian-Yu; Cheng, Jin-Pei Inorganic Chemistry 46
2006 A facile experimental method to determine the hydride affinity of polarized olefins in acetonitrile Zhu, XQ; Zhang, M; Liu, QY; et al. Angewandte Chemie-International Edition 45