学 历
博士

职 称
教授

所属部门
微生物学系

招生专业
微生物学（博士、硕士），生物工程（专业硕士）

联系方式
电话: E-mail: zhyou21cn@sdau.edu.cn

个人简介

杨正友，男，博士，教授，博士生导师。1995年毕业于山东农业大学，获学士学位，并留校任教至今。2001年至2006年到韩国留学，在韩国国立庆尚大学研究生院应用生命科学部，先后获得硕士学位与博士学位，主要从事食品微生物的研究，包括食品卫生与安全、益生菌定向筛选与功能研究等。近年来先后主持承担了国家自然科学基金、国家转基因重大专项、教育部留学回国人员科研启动基金、山东省自然科学基金、国家863计划等多项课题。在《Journal of Agricultural and Food Chemistry》、《Scientific Reports》、《Food Control》、《Journal of Food Protection》、《Journal of Microbiology and Biotechnology》等刊物上发表论文30多篇。

教学工作

1.本科生《普通微生物学》，《食品微生物学》《食品与工业微生物技术》等
2.研究生《微生物学研究进展》、《生物安全与控制》、《食品与工业微生物技术》等

研究方向

1.食品卫生与安全：研究和建立快速免疫检测技术和分子生物学检测技术，实现食品微生物安全控制；研究真菌毒素的快速检测方法，建立快速真菌毒素的检测体系。
2.益生菌定向筛选与功能开发研究：研究和建立新型益生菌定向筛选模型，实现调血脂/血糖、降血压、抗氧化、抗过敏和免疫调节等生理功能益生菌的高效筛选；研究益生菌的吸附和定植技术、功能基因锚定技术、益生性状整合技术、高密度培养、制备和活性保护技术。
3.环境中（农田、污水）持久性有机污染物降解工程菌定向筛选与基因工程菌构建研究：以农田持久性有机污染物为对象定向筛选工程细菌，克隆降解关键酶基因，并利用同源重组、染色体无标记整合、启动子调控等方法，研究不含外源标记、生态安全的工程菌。

科研项目

1. 国家自然科学基金《基于纳米肽适配体的农药多残留高灵敏快速免疫分析方法的研究》，2018-2021，主持。
2.农业部《国家农产品质量安全风险评估项目》，2016-2018，主持。
3.国家自然科学基金《基于双功能抗体的农药多残留免疫检测技术的基础研究》，2013-2016，主持。
4.国家自然科学基金《噬菌体展示肽库筛选农药通用结构半抗原的肽模拟物及其在农药多残留免疫检测中的应用研究》，2010-2012， 主持。
5.教育部留学回国人员科研启动基金《有机磷农药多残留快速免疫检测技术的研究》，2010-2012，主持。
6.山东省自然科学基金《 利用噬菌体展示技术筛选和鉴定展青霉素的模拟抗原表位及其在展青霉素免疫分析中的应用研究 》，2010-2013，主持。
7.国家转基因重大专项《转基因生物快速、精确和高通量检测技术的系统研究与产品开发》，2009-2011，主持。
8.863计划项目《化学农药多残留免疫快速检测技术系统性研究与产品开发》，2006-2009，参与。

科研奖励


荣誉称号


研究专利

1.特异性检测转基因大豆DP-356043的标准质粒分子及其应用，专利号：ZL0.1。
2.用于转基因玉米Mon810检测的标准质粒分子及其构建方法，专利号：ZL0.7。

发表论文


Fengchun Zhao, Qiang Shen, Hunmin Wang, Xiao Han andZheng You Yang*. Development of a rapid magnetic bead-based immunoassay for sensitive detection of zearalenone. Food Control, 2017, 79: 227-233.
Yufang Liu, Zhao,Fengchun Zhao, JiyieLiu, Xiao Han, Yongxin Zhang,Zheng You Yang*.Selection of cholesterol-lowering lactic acid bacteria and its effects on rats fed with high-cholesterol diet.Current Microbiology2017, 74(5), 623-631.
Fengchun Zhao, Hunmin Wang, Xiao Han andZheng You Yang*.Development and comparative study of chemosynthesized antigen and mimotope-based immunoassays for class-specific analysis of O,O-dimethyl organophosphorus pesticides. Scientific Reports, 2016, 6: 37640.
Fengchun Zhao, Yuan Tian, Hunmin Wang, Jiye You, Xiao Han andZheng You Yang*. Development of a biotinylated broad-specificity single-chain variable fragment antibody and a sensitive immunoassay for detection of organophosphorus pesticides. Analytical and Bioanalytical Chemistry, 2016, 408: 6423-6430.
Huimin Wang, Fengchun Zhao, Xiao Han andZheng You Yang*.Production and characterization of a biotinylated single-chain variable fragment antibody for detection of parathion-methyl. Protein Expression and Purification, 2016, 126: 1-8.
Hui Qu , Longyu Zhao, Fengchun Zhao, Yufang Liu andZheng You Yang*. Biocontrol of gray mold decay in pear by bacillus amyloliquefaciens strain BA3 and its effect on postharvest quality parameters. Polish Journal of Microbiology, 2016, 1: 171-176.
Fengchun Zhao, Chunyang Hu, Hunmin Wang, Longru Zhao andZheng You Yang*. Development of a MAb-based immunoassay for the simultaneous determination of O,O-diethyl and O,O-dimethyl organophosphorus pesticides in vegetable and fruit samples pretreated with QuEChERS. Analytical and Bioanalytical Chemistry, 2015, 407: 8959-8970.
Yanling Li,Fengchun Zhao,Longyu Zhao,Zhengyou Yang*.Development of a Broad-Specificity Immunoassay for Determination of Organophosphorus Pesticides Using Dual-Generic Hapten Antigens.Food Analytical Methods.2015, 8: 420-427.
Zheng-You Yang*, Won-Bo Shim, Kyeong-Yeol Kim, and and Duck-Hwa Chung. Rapid Detection of Enterotoxigenic Clostridiumperfringens in Meat Samples Using Immunomagnetic Separation Polymerase Chain Reaction (IMS-PCR).Journal of Agricultural and Food Chemistry.2010, 58(12): 7135–7140.
10.Zheng-You Yang*, Won-Bo Shim, Min-Gon Kim, Kyu-Ho Lee, Keun-Sung Kim, Kwang-Yup Kim, Cheol-Ho Kim, Sang-Do Ha, and Duck-Hwa Chung. Production and Characterization of Monoclonal and Recombinant Antibodies against Antimicrobial Sulfamethazine.Journal of Microbiology and Biotechnology.2007,17(4):571-578.
11.Zheng-You Yang*, Anna Yu Kolosova, Won-Bo Shim and Duck-Hwa Chung. Development of Monoclonal Antibodies against Pirimiphos-methyl and Their Application to IC-ELISA.Journal of Agricultural and Food Chemistry.2006, 54(13): 4551-4556.
12.Zheng-You Yang*, Won-Bo Shim, Ji-Hun Kim, Seon-Ja Park, Sung-Jo Kang, Baik-Sang Nam, and Duck-Hwa Chung. Detection of Aflatoxin-Producing Molds in Korean Fermented Foods and Grains by Multiplex PCR.Journal of Food Protection.2004, 67(11): 2622-2626.
13.Won-Bo Shim,Zheng-You Yang, Ji-Young Kim, Jin-Gil Choi, Jung-Hyun Je, Sung- Jo Kang, Anna Yu Kolosova, Sergei A. Eremin, and Duck-Hwa Chung. Immunochromatography Using Colloidal Gold-antibody Probe for the Detection of Atrazine in Water Samples.Journal of Agricultural and Food Chemistry.2006, 54(26): 9728-9734.
14. Won-Bo Shim,Zheng-You Yang, Jung-Sook Kim, Ji-Young Kim, Jin-Gil Choi, Sung- Jo Kang, Gun-Jo Woo, Young-Chul Chung, Sergei A. Eremin, and Duck-Hwa Chung. Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples.Journal of Microbiology and Biotechnology.2007, 17(10): 1629-1637.
15. Anna Yu Kolosova, Won-Bo Shim,Zheng-You Yang, Sergei A. Eremin, Duck-Hwa Chung. Direct competitive ELISA based on a monoclonal antibody for detection of aflatoxin B₁. Stabilization of ELISA kit components and application to grain samples.Anal Bioanal Chem.2006, 384: 286-294.
16. Won-Bo Shim, Anna Yu. Kolosova, Yoon-Jung Kim,Zheng -You Yang, Seon-Ja Park, Sergei A. Eremin, In-Seon Lee, and Duck-Hwa Chung. Fluorescence polarization immnoassay based on a monoclonal antibody for detection of ochratoxin A.International Journal of Food Science and Technology.2004, 39: 829-837.
17. Won-Bo Shim, Jin-Gil Choi, Ji-Young Kim，Zheng-You Yang, Kyu-Ho Lee, Min-Gon Kim, Sang-Do Ha, Keun-Sung Kim, Kwang-Yup Kim, Cheol-Ho Kim, Kwang-Soo Ha, Sergei A. Eremin, and Duck-Hwa Chung. Production of Monoclonal Antibody Against Listeria monocytogenes and Its Application to Immunochromatography Strip Test.Journal of Microbiology and Biotechnology.2007, 17(7):1152-1161.
18. Won-Bo Shim, Jin-Gil Choi, Ji-Young Kim，Zheng-You Yang, Kyu-Ho Lee, Min-Gon Kim, Sang-Do Ha, Keun-Sung Kim, Kwang-Yup Kim, Cheol-Ho Kim, Kwang-Soo Ha, Sergei A. Eremin, and Duck-Hwa Chung. Enhanced Rapidity for Qualitative Detection ofListeria monocytogenesUsing an Enzyme-Linked Immunosorbent Assay and Immunochromatography Strip Test Combined with Immunomagnetic Bead Separation.Journal of Food Protection.2008, 71(2):781-789.