杨健 教授
硕士招生类型：学术型
硕士招生专业：基础医学
博士招生类型：学术型
博士招生专业：基础医学
所在院系：医学院
联系方式：Jy279@tongji.edu.cn
课题组链接：
研究方向
主要任职
科研业绩

干细胞生物学、体细胞重编程、心脏发育与分化
Stem cell biology, somatic cell reprogramming, heart development and differentiation
同济大学医学院教授，复旦大学博士，英国爱丁堡大学，剑桥大学和威康桑格研究所博士后和专职研究员，中国生物医学工程学会会员 Stem Cell Reports审稿专家；
Professor of Tongji University, School of Medicine ；
Awarded PhD. by Fudan University；
Postdoctoral Research Associate at Edinburgh University, Cambridge University and Staff Scientist at Wellcome Trust Sanger Institute；
Member of Chinese Society of Biomedical Engineering；
Peer Reviewer of Stem Cell Reports
1.从小鼠8细胞期胚胎和单个卵裂球分离，建株一种新型的胚胎干细胞，扩增潜能多能干细胞(Expanded potential stem cell, EPSC)。这些干细胞具有向胚胎和胚外组织分化的潜能，为研究小鼠早期胚胎发育提供了新的细胞来源和工具。
2.首先建立了体外将na?ve 胚胎干细胞（embryonic stem cells, ESCs）转化为Primed 上胚层干细胞（epiblast stemcells, EpiSCs）的培养系统，随后，阐明了Jak/STAT3和维甲酸信号通路在EpiSC和体细胞重编程中的重要作用和机制。
3. 运用CRISPRa对小鼠全基因组进行筛选能重编程EpiSC的因子，发现了一些新的因子，并证实它们具有重编程功能。重点研究了Sall1, Nanog这两个基因，发现两者在EpiSC和成纤维细胞重编程中有协同作用，进一步发现并证实了它们下游各自的靶基因。
1. We established a new type of embryonic stem cell-Expanded potential stem cell (EPSCs) from mouse 8C embryo and single blastomere. The EPSCs can contribute to both embryonic and extraembryonic tissues, which provide a new tool and cell resource for the study of mouse early embryo development.
2. We first developed an in vitro culture system to convert na?ve embryonic stem cells (ESCs) into primed epiblast stem cells (EpiSCs), then clarified roles of Jak/STAT3 and Retinoic Acid signaling pathways in reprogramming primed EpiSCs and somatic cells into na?ve iPSCs and the related molecular mechanism.
3. By mouse whole genome CRISPRa screen, we identified and validated some new reprogramming factors. Particularly, we discovered that SALL1 and NANOG not only facilitate EpiSCs and fibroblasts reprogramming, but also have synergistic effects. Furthermore, we revealed the downstream targets of SALL1 and NANOG in reprogramming.
目前承担的项目：
1.国家自然科学基金基础科学中心：重大心脏病的起源和干预，骨干；
2.国家自然科学基金面上项目：Sall1调控多能干细胞na?ve和primed状态转换的机制研究，负责人；
3.国家自然科学基金重点项目：蛋白激酶介导的心肌细胞增殖在心肌损伤修复中的作用及机制研究，骨干；
4.中国医学科学院心律的起源与调控创新单元，骨干
发表的代表性文章：
1.Nicholas Bredenkamp, Jian Yang, James Clarke, Giuliano Giuseppe Stirparo, Ferdinand von Meyenn, Sabine Dietmann, Duncan Baker, Rosalind Drummond, Yongming Ren, Chuman Wu, Maria Rostovskaya, Sarah Eminli-Meissner, Austin Smith, and Ge Guo. Wnt inhibition facilitates RNA-mediated reprogramming of human somatic cells to na?ve pluripotency. Co-first author, Stem Cell Reports, 2019, 13(6): 1083-1098；
2.Jian Yang, Sandeep S. Rajan, Mathias J Friedrich, Guocheng Lan, Xiangang Zou, Hannes Ponsting, Dimitrios A Garyfallos, Pentao Liu, Allan Bradley, and Emmanouil Metzakopian. Genome-scale CRISPRa screen identifies novel factors for cellular reprogramming. Co-first author, Stem Cell Reports, 2019, 12(4):754-771；
3.Jian Yang, David Ryan, Guocheng Lan, Xiangang Zou and Pentao Liu*. Establishment in vitro of Expanded Potential Stem Cells from mouse preimplantation embryos and embryonic stem cells. Co-corresponding author, Nature Protocols, 2019, 14, 350-378；
4.Jian Yang, David J. Ryan, Wei Wang, Jason Cheuk-Ho Tsang, Guocheng Lan, Hideki Masaki, Xuefei Gao, Liliana Antunes, Yong Yu, Zhexin Zhu, Juexuan Wang, Aleksandra A. Kolodziejczyk, Lia S. Campos, Cui Wang, Fengtang Yang, Zhen Zhong, Beiyuan Fu, Melanie Eckersley-Maslin, Michael Woods, Yosuke Tanaka, Xi Chen, Adam C. Wilkinson, James Bussell, Jacqui White, Ramiro Ramirez-Solis, Wolf Reik, Berthold G?ttgens, Sarah A. Teichmann, Patrick P. L. Tam, Hiromitsu Nakauchi, Xiangang Zou, Liming Lu and Pentao Liu. Establishment of murine expanded potential stem cells. Co-first author, Nature, 2017, 550, 393-397；
5.Jian Yang, Wei Wang, Jolene Ooi, Lia Campos, Liming Lu and Pentao Liu. Signaling through retinoic acid receptors is required for reprogramming of both MEFs and EpiSCs to iPSCs. Stem Cells, 2015, 33(5): 1390-1404；
6.Jian Yang, Anouk L. van Oosten, Thorold W. Theunissen, Ge Guo, Jose C.R. Silva and Austin Smith Stat3 Activation Is Limiting for Reprogramming to Ground State Pluripotency. Cell Stem Cell, 2010, 7(3): 319-328；
7.Jindun Zhang, Xuefei Gao, Jian Yang, Xiaoying Fan, Wei Wang, Yanfeng Liang, Lihong Fan, Hongmei Han, Xiaorong Xu, Fuchou Tang, Siqin Bao, Pentao Liu and Xihe Li. Xist intron 1 repression by TALE designer transcriptional factor improves somatic cell reprogramming in mice. Stem Cells, 2019, 37(5): 599-608；
8.Xuefei Gao, Jian Yang, Jason Tsang, Jolene Ooi, Donghai Wu and Pentao Liu. Reprogramming to Pluripotency Using Designer TALE Transcription Factors Targeting Enhancers. Stem Cell Reports, 2013, 1(2): 183-197；
9.Wei Wang, Jian Yang, Hui Liu, Dong Lu, Xiongfeng Chen , Zenon Zenonos, Lia S. Campos, Roland Rad, Ge Guo, Shujun Zhang, Allan Bradley, and Pentao Liu. Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog .Proc Natl Acad Sci U.S.A, 2011, 108(45): 18283-18288;
10.Ge Guo, Jian Yang, Jennifer Nichols, John Simon Hall, Isobel Eyres, William Mansfield and Austin Smith. Klf4 reverts developmentally programmed restriction of ground state pluripotency. Development, 2009, 136(7): 1063-1069;
11.Mia Buehr, Stephen Meek, Kate Blair, Jian Yang, Janice Ure, Jose Silva, Renee McLay, John Hall, Qi-Long Ying and Austin Smith. Capture of Authentic Embryonic Stem Cells from Rat Blastocysts. Cell, 2008, 135(7): 1287-1298.