下调miR-27a表达对三阴乳腺癌细胞增殖、迁移和侵袭的影响

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摘要/Abstract

摘要： 目的·探讨下调miR-27a表达对三阴乳腺癌(triple-negative breast cancer，TNBC)细胞系MDA-MB-453增殖、迁移和侵袭的影响。方法·采用实时荧光定量PCR方法检测68例TNBC患者癌组织及癌旁组织标本的miR-27a表达，分析miR-27a表达与患者临床特征的关系；并检测TNBC细胞系MDA-MB-453及正常乳腺上皮细胞MCF-10A中miR-27a表达的差异。在MDA-MB-453细胞中转染miR-27a抑制物（miR-27a inhibitor），采用CCK-8实验、Transwell小室法检测miR-27a inhibitor转染组（miR-27a inhibitor组）与miR-27a阴性对照片段转染组（NC组）TNBC细胞增殖、迁移和侵袭能力的差异。蛋白质印迹法检测转染miR-27a抑制物对MDA-MB-453细胞泛素连接酶FBW7（F-box and WD repeat domain-containing 7）及Egl 9同源物2（Egl nine homolog 2，EGLN2）蛋白表达的影响。结果·TNBC组织中miR-27a表达明显高于癌旁组织，差异有统计学意义（PPPPPP结论·miR-27a在TNBC组织及细胞株中表达升高；下调miR-27a表达可以抑制TNBC细胞MDA-MB-453的增殖、迁移和侵袭能力，这可能与其下调后FBW7蛋白表达增加、EGLN2蛋白表达减少有关。
关键词: miR-27a, 三阴乳腺癌, FBW7, Egl 9同源物2, 增殖, 迁移, 侵袭
Abstract:
Objective · To investigate the effect of miR-27a down-regulation on proliferation, migration and invasion in triple-negative breast cancer (TNBC) cell line MDA-MB-453. Methods · Sixty-eight pairs of breast cancer tissues and para-carcinoma tissues the TNBC cancer patients were harvested for the study. The differential of miR-27a in the collected tissues, MDA-MB-453 cell line and normal breast epithetial cell line MCF-10A were detectedquantitative real-time PCR (qPCR). The relationship between miR-27a and the clinical characteristics of TNBC patients was analyzed. miR-27a inhibitor was transfected into MDA-MB-453 cell line. CCK-8 assay and Transwell chamber test were used to detect the difference in proliferation, migration and invasion of MDA-MB-453 cells between miR-27a inhibitor group and negative control group (NC group). The levels of F-box and WD repeat domain-containing 7 (FBW7) and Egl nine homolog 2 (EGLN2) protein were examinedWestern blotting. Results · The of miR-27a in TNBC tissues was significantly higher than that in para-carcinoma tissues (PPPPPPPConclusion · The of miR-27a in TNBC tissues and TNBC cell line increases. Down-regulation of miR-27a can inhibit the proliferation, migration and invasion of MDA-MB-453 cells, which may be through increasing FBW7 and decreasing EGLN2 .
Key words: miR-27a, triple-negative breast cancer (TNBC), F-box and WD repeat domain-containing 7 (FBW7), Egl nine homolog 2 (EGLN2), proliferation, migration, invasion

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