摘要/Abstract
摘要： 目的 ·探究短期奥氮平给药后所引起的 SD大鼠体内代谢产物或代谢通路的变化。方法 ·采用 5周龄雌性 SD大鼠 20只，随机分为 2组，分别为空白对照组和奥氮平组。给药期间，监测 2组大鼠的体质量和摄食量。给药结束后，采集肝组织，并运用液相色谱 -质谱联用技术对肝组织中的代谢产物进行检测，后续转换原始数据，导入 MetaboAnalysis平台进行分析。结果 ·短期奥氮平给药会引起体质量、摄食量的显著增加以及糖耐量受损。代谢组学结果提示奥氮平组大鼠肝组织中初级胆汁酸和 9-氧代十八碳二烯酸（9-oxo-octadecadienoic acid, 9-OxoODE）水平显著高于空白对照组。结论 ·短期奥氮平给药会导致 SD大鼠体内初级胆汁酸生物合成过程的显著增强。
关键词: 奥氮平, 代谢组学, 肝组织, 液相色谱 -质谱联用
Abstract:
Objective · To observe the changes in metabolites and metabolic pathways in SD rats after olanzapine treatment. Methods · Twenty female SD rats were randomly assigned to two groups, control group and olanzapine treatment group, respectively. During the treatment period, body weight and food intake of two groups were monitored. At the end of treatment, the liver tissues were collected and LC/MS technique was applied to detect metabolites in liver tissue. Subsequently, the raw data were converted for further analysis on the MetaboAnalysis platform. Results · Short-term olanzapine administration resulted in significant increases in body mass, food intake, and impaired glucose tolerance. Metabonomics results indicated rats olanzapine treatment group had a significantly higher level of primary bile acid and 9-OxoODE (9-oxo-octadecadienoic acid) in liver tissue than those control group. Conclusion · Primary bile acid biosynthesis process is significantly enhanced after short-time olanzapine treatment.
Key words: olanzapine, metabonomics, liver tissue, liquid chromatography-mass spectrometry
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