摘要/Abstract

摘要： 目的 ·比较问号钩端螺旋体 56606v株和 56606a株对小鼠巨噬细胞的感染情况，探究致病性钩端螺旋体的致病机制。方法 ·用问号钩端螺旋体强毒株 56606v株和减毒株 56606a株分别感染 C57BL/6小鼠腹腔巨噬细胞后，对样本进行免疫荧光染色，观察巨噬细胞对钩端螺旋体的吞噬及清除能力，并通过 realtime-PCR检测感染后巨噬细胞的细胞因子的表达。结果 ·免疫荧光染色和 realtime-PCR结果均发现在感染 72 h后，56606v株仍能在巨噬细胞中存活，而 56606a株不能在巨噬细胞中存活；巨噬细胞对 56606v株的吞噬率高于对 56606a株的吞噬率，而感染 56606v株后细胞因子的表达低于感染 56606a株后的水平。结论 ·问号钩端螺旋体强毒株 56606v株能在巨噬细胞中存活，并引起较低的炎症反应，可借此逃避免疫细胞的杀伤从而引发疾病；而减毒株 56606a株被巨噬细胞吞噬后则被清除，且引发较强的炎症反应，易被机体免疫细胞清除，从而不引起疾病。
关键词: 钩端螺旋体, 巨噬细胞, 吞噬, 炎症反应, 免疫逃逸
Abstract:
Objective · To compare the response and consequence of momacrophages infectedLeptospira interrogans 56606v and 56606a, and explore the mechanisms of pathogenic Leptospira to cadisease. Methods · Peritoneal macrophages of C57BL/6 mice were infectedpathogenic Leptospira 56606v and non-pathogenic Leptospira 56606a respectively. Immunofluorescence staining was performed to observe phagocytosis and clearance of Leptospira after infection, and realtime-PCR was used to determine cytokine production of macrophages. Results · After 72-hour infection, strain 56606v exhibited a lower phagocytic rate but survived after incubation with peritoneal macrophages compared with strain 56606a, which showed a higher phagocytic rate but was cleared at that time point. Additionally, cytokine production of macrophages incubated with 56606v was lower than that with 56606a. Conclusion · Leptospira interrogans strain 56606v can survive in macrophages, which may contribute to evasion phagocytic clearance and lead to disease, while strain 56606a can be cleared, which implicates a lower pathogenicity.
Key words: Leptospira interrogans, macrophage, phagocytosis, inflammatory response, immune surveillance


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