摘要/Abstract

摘要： 目的 ·探讨利拉鲁肽对高糖诱导人脐静脉内皮细胞（ human umbilical vein endothelial cells，HUVECs）miR-146b-3p及白细胞介素 -6（interleukin-6，IL-6）、肿瘤坏死因子 α（tumor necrosis factor-α，TNF-α）、环氧化酶 -2(cyclooxygenase-2，COX-2)表达的影响。方法 ·高糖组和对照组 HUVECs分别以高浓度葡萄糖（ 25 mmol/L）及正常浓度葡萄糖（ 7 mmol/L）处理 24 h，再以利拉鲁肽分别干预上述 2组细胞。定量聚合酶链反应（ quantitative polymerase chain reaction，qPCR）检测各组 miR-146b-3p、IL-6、TNF-α、COX-2的表达变化。抑制 miR-146b-3p后观察炎症因子及 COX-2的表达变化。结果 ·在高糖诱导的 HUVECs中，miR-146b-3p的表达下降， IL-6、TNF-α及 COX-2的表达均升高。抑制 miR-146b-3p表达会显著升高高糖诱导的 HUVECs中 IL-6、TNF-α及 COX-2的表达。利拉鲁肽可显著升高高糖诱导的 HUVECs中 miR-146b-3p的表达，降低 IL-6、TNF-α及 COX-2的表达。结论 ·在高糖环境下，利拉鲁肽可能通过升高 miR-146b-3p的表达而降低 IL-6、TNF-α及 COX-2的表达，从而改善炎症和血管内皮功能。
关键词: 利拉鲁肽, miR-146b-3p, 炎症因子, 环氧化酶 -2, 糖尿病
Abstract:
Objective · To investigate the effects of liraglutide on glucose induced of miRNA-146b-3p, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2) in human umbilical vein endothelial cells (HUVECs). Methods · HUVECs were grown in the medium with glucose of high concentration (25 mmol/L) or normal concentration (7 mmol/L) for 24 h, then stimulated with liraglutide. Quantitative polymerase chain reaction (qPCR) was performed to detect the of miR-146b-3p, IL-6, TNF-α and COX-2. The of IL-6, TNF-α and COX-2 were detected after HUVECs were transfected with anti-miR-146b-3p. Results · The of miR-146b-3p was decreased in high glucose induced cells, while the of IL-6, TNF-α and COX-2 were increased. Silenced of miR-146b-3p increased the of IL-6, TNF-α and COX-2. Liraglutide increased the of miR-146b-3p and decreased the of IL-6, TNF-α and COX-2 in high glucose induced cells. Conclusion · Liraglutide may relieve inflammation and improve vascular endothelial function in hyperglycemia condition through regulating miR146b-3p to decrease of IL-6, TNF-α and COX-2.
Key words: liraglutide, miR-146b-3p, inflammatory factor, cyclooxygenase-2 (COX-2), diabetes


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