导师姓名： 亓磊 专业代码： 100100
性别： 男 专业名称： 基础医学
培养单位： 附属仁济医院 三级学科/
专业领域：
导师类型： 博士生导师 专业学位导师：
联系方式： leiqi01@gmail.com
邮编： 邮箱地址： leiqi01@gmail.com

研究方向 (点击浏览详细信息)
合成生物学基因（组）编辑

社会任职
美国冷泉港实验室培训课程授课教授
国际合成生物学年会（SEED）董事
加州生物杠杆（Biofulcrum）项目董事会成员
美国科学院：美日科学学术（JAFOS）研讨会组织者
美国科学院：美德工程学术（GAFOE）前沿研讨会组织者
美国蛋白质学会（Protein Society）成员
美国微生物学会（ASM）荣誉会员
美国生物工程学会（BMES）成员
国际干细胞研究学会（ISSCR）会员

科研项目 项目编号项目名称课题来源起止年月批准经费承担职责
** 大规模CRISPR/Cas9基因组编辑研究干细胞多能分化性 国家自然科学基金面上项目 2018-01~2021-12 60万元 课题负责人

学术论文 作者论文标题期刊名出版年卷期页码
Takei Y, Shah S, Harvey S, Qi LS, Cai L Multiplexed dynamic imaging of genomic loci in single cells by combined CRISPR imaging and DNA sequential FISH Biophys J 2017 112, 1773–1776
Du D, Roguez A, Gordon DE, Chen M, Chen SH, Shales M, Shen JP, Ideker T, Mali P, Qi LS* & Krogan NJ* （通讯作者） Genetic interaction mapping in mammalian cells using CRISPR interference Nat Methods 2017 14(6):577-580
Shen JP, Zhao D, Sasik R, Luebeck K, Birmingham A, Bojorquez-Gomez A, Licon K, Klepper K, Pekin D, Beckett AN, Sanchez KS, Thomas A, Kuo CC, Du D, Roguez A, Lewis NE, Chang AN, Kreisberg JF, Krogan NJ, Qi LS, Ideker T, Mali P Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions Nat Methods 2017 14(6):573-576
Wang H, La Russa M, Qi LS （通讯作者） CRISPR-Cas9 in Genome Editing and Beyond Annu. Rev. Biochem 2016 85:22.1–22.38
Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS （通讯作者） Complex Transcriptional Modulation with Orthogonal and Inducible dCas9 Regulators Nat. Method 2016 13:1043-1049
Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Chan AH, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs Cell Stem Cell 2016 18: 541–543
Wang F, Qi LS （通讯作者） Applications of CRISPR Genome Engineering in Cell Biology Trends Cell Biol 2016 26:875-888
Dominguze AA, Lim WA, Qi LS （通讯作者） Beyond Editing: Repurposing CRISPR-Cas9 for Precision Genome Regulation and Interrogation Nat. Rev. Mol. Cell. Biol 2016 17, 5–15
Xiong X, Chen M, Lim WA, Zhao D, Qi LS （通讯作者） CRISPR/Cas9 for Human Genome Engineering and Disease Research Annu. Rev. Genomics Hum. Genet 2016 17:131-54
Peters JM, Colavin A, Shi H, Czarny ML, Larson MH, Wong S, Hawkins JS, Lu CHS, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS* （通讯作者）, Huang KC*, Gross CA* A Comprehensive, CRISPR-Based Approach to Functional Analysis of Essential Genes in Bacteria Cell 2016 165: 1493-1506
Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song JS, Ramalho-Santos M YAP Induces Human Na?ve Pluripotency Cell Reports 2016 14: 2301–2312
La Russa MF and Qi LS The New State of the Art: Cas9 for Gene Activation and Repression Mol. Cell Biol 2015 35(22): 3800-3809
Zalatan JG, Lee ME, Gilbert LA, Almeida R, Tsai JC, Whitehead EH, Weissman JS, Dueber JE, Qi LS* （通讯作者）, Lim WA* Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds Cell 2015 160(1-2):339-350
Sokolik C, Liu Y, Bauer D, McPherson J, Broeker M, Heimberg G, Qi LS, Sivak DA, Thomson M Transcription Factor Competition Allows Embryonic Stem Cells to Distinguish Authentic Signals from Noise Cell Systems 2015 1(2): 117-129
Peters JM, Silvis MR, Zhao D, Hawkins JS, Gross CA*, Qi LS* （通讯作者） Bacterial CRISPR: Accomplishments and Prospects Curr. Opin. Microbiol 2015 27: 121-126
Yu C, Liu Y, Ma T, Xu S, Liu K, Zhang Y, La Russa M, Xie M, Ding S*, Qi LS* （通讯作者） Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells Cell Stem Cell 2015 16(2):142-147
Liu H, Wei Z, Dominguez A, Li Y, Wang X, Qi LS （通讯作者） CRISPR-ERA: A Comprehensive Designer Tool for CRISPR-Mediated Gene Editing, Repression and Activation Bioinformatics 2015 31(22):3676-8
Hawkins JS, Wong S, Peters JM, Almeida R, Qi LS （通讯作者） Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi) Methods Mol. Biol 2015 1311: 349-362
Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation Cell 2014 159(3):647-661
Qi LS* （通讯作者）, Arkin AP* A Versatile Platform for Microbial Engineering using Synthetic Noncoding RNAs Nat. Rev. Microbiol 2014 12(5):341-354
Ji W, Lee D, Wong E, Dadlani P, Dinh D, Huang V, Kearns K, Teng S, Chen S, Haliburton J, Heimberg G, Heineike B, Ramasubramanian A, Stevens T, Helmke KJ, Zepeda V, Qi LS, Lim WA Specific Gene Repression by CRISPRi System Transferred through Bacterial Conjugation ACS Synth Biol 2014 3(12):929-31
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD A Versatile Protein Tagging System for Signal Amplification in Single Molecule Imaging and Gene Regulation Cell 2014 159(3):635-646
Qi LS* （通讯作者）, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA Repurposing CRISPR as An RNA-guided Platform for Sequence-Specific Control of Gene Expression Cell 2013 152(5):1173-1183
Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes Cell 2013 154(2):442-451
Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS* （通讯作者）, Huang B* Dynamic Imaging of Genomic Loci in Living Human Cells by An Optimized CRISPR/Cas System Cell 2013 155(7):1479-1491
Larson MH, Gilbert LA, Wang X, Lim WA, Weissman JS, Qi LS （通讯作者） CRISPR Interference (CRISPRi) for Sequence-Specific Control of Gene Expression Nat. Protoc 2013 8(11): 2108-2196
Mutalik VK*, Qi LS* （共同第一作者）, Guimaraes JC, Lucks JB, Arkin AP Rationally Designed Families of Orthogonal RNA Regulators of Translation Nat. Chem. Biol 2012 8(5):447-454
Qi LS, Haurwitz RE, Shao WJ, Doudna JA, Arkin AP RNA Processing Enables Predictable Programming of Gene Expression Nat. Biotechnol 2012 30(10):1002-1006
Liu CC*, Qi LS* （共同第一作者）, Lucks JB, Segall-Shapiro TH, Wang D, Mutalik VM, Arkin AP An Adaptor from Translational to Transcriptional Control Enables Predictable Assembly of Complex Regulation Nat. Methods 2012 9(11):1088-1094
Qi LS, Lucks JB, Liu CC, Mutalik VK, Arkin AP Engineering Naturally Occurring Trans-Acting Non-Coding RNAs to Sense Molecular Signals Nucleic Acids Res 2012 40(12):5775-5786
Lucks JB*, Qi LS* （共同第一作者）, Mutalik VK, Wang D, Arkin AP Versatile RNA-Sensing Transcriptional Regulators for Engineering Genetic Networks Proc. Natl Acad. Sci. USA 2011 108(21):8617-8822
Liu CC, Qi LS, Yanofsky C, Arkin AP Regulation of Transcription by Unnatural Amino Acids Nat. Biotechnol 2011 29(2):164-168
Lucks JB, Qi LS, Whitaker WR, Arkin AP Toward Scalable Parts Families for Predictable Design of Biological Circuits Curr. Opin. Microbiol 2008 11(6):567-573