摘要/Abstract

microRNA是一段长约为18~24个核苷酸的内源性非编码单链RNA. 最新研究发现: 许多疾病和肿瘤的发生与microRNA的表达水平息息相关, 且microRNA有望成为新型肿瘤标志物及癌症治疗的新目标. 因此, 发展高灵敏度、高特异性及简单快速的microRNA分析检测方法对于生物医学研究和癌症的早期诊断具有重要的意义. 表面增强拉曼光谱(SERS)技术由于具有灵敏度高、检测速度快、指纹识别、水干扰小等独特优势, 在癌症的早期诊断领域具有很大的应用价值. 作者综述了SERS技术在microRNA检测方面的最新研究进展, 分析了该技术在生物检测中亟待解决的关键问题和挑战, 并对其未来的发展前景进行了展望.
关键词: 表面增强拉曼光谱, microRNA, 肿瘤标志物
microRNA is an endogenous non-coding single-stranded RNA with a length of about 18~24 nucleotides. The latest research has found that the occurrence of many diseases and tumors is closely related to the level of microRNA expression, and microRNA is expected to become a new tumor marker and a new target for cancer treatment. Therefore, the development of high sensitivity, high specificity, and simple and rapid microRNA detection methods is greatly significant for biomedical research and early diagnosis of cancer. Surface-enhanced Raman spectroscopy (SERS) technique has great application value in the field of early cancer diagnosis due to its unique advantages such as high sensitivity, fast detection speed, fingerprint recognition, and low water interference. The latest research progress of SERS technique in microRNA detection is summarized in this review. Finally the main challenges of SERS technique in bioassay are discussed and the future development trend is proposed.
Key words: surface-enhanced Raman spectroscopy, microRNA, tumor marker


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