摘要/Abstract

开发了两种水溶性的反应型氟硼二吡咯（BODIPY）荧光探针Ac-Phe-BODIPY和Ac-BODIPY，并将其用于活性氧物种（ROS）和活性氮物种（RNS）中过氧亚硝酸盐（ONOO^-）的检测，发现其具有灵敏度高、检测限低和对ONOO^-的选择性好等优点.Ac-Phe-BODIPY的水解产物通过柱层析法分离得到，并用¹H NMR和ESI-MS表征水解产物为BODIPY-1，证明了光致电子转移（PET）机理与密度泛函理论（DFT）的计算结果相符合.
关键词: 氟硼二吡咯(BODIPY)荧光探针, 过氧化亚硝酸盐, 水溶性, 荧光检测
Two reaction-based water-soluble boron-dipyrromethene (BODIPY) fluorescent probes, Ac-Phe-BODIPY and Ac-BODIPY, were developed for peroxynitrite (ONOO^-) measurement. High sensitivity, low detection limit and excellent selectivity toward ONOO^- were obtained for determining reactive oxygen species (ROS) and reactive nitrogen species (RNS). The reaction-based probe Ac-Phe-BODIPY was hydrolyzed into BODIPY-1 which was isolated by column chromatography and proved by ¹H NMR and ESI-MS. The characterization results revealed the photoinduced electron transfer (PET) mechanism which was simultaneously in agreement with density functional theory (DFT) calculation.
Key words: boron-dipyrromethene (BODIPY) fluorescent probe, peroxynitrite, water-soluble, fluorescent detection


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