摘要/Abstract

猪胃粘膜蛋白酶具有催化芳香胺和2-氨基苯甲醛环化缩合反应的能力，在甲醇水溶液中，以良好的收率（41%~95%）合成了一系列四氢喹唑啉类化合物.通过优化酶促反应的溶剂、温度、酶用量和底物比例来提高胃蛋白酶的催化活性.该方法可作为四氢喹唑啉衍生物化学合成的替代方法，也进一步拓展了胃蛋白酶非天然催化活性的应用范围.
关键词: 胃蛋白酶, 非天然催化活性, 四氢喹唑啉, 生物催化
Pepsin from porcine gastric mucosa has a non-natural ability to catalyze the cyclocondensation of aromatic amines and 2-aminobenzaldehyde. The corresponding tetrahydroquinazolines were obtained in good yields (41%~95%) in aqueous methanol solution. The catalytic activity of pepsin was evaluated by investigating solvent, temperature, enzyme loading, and substrate ratio in the enzyme-catalyzed reaction. Herein, a potential biocatalytic approach as an alternative to chemical synthesis for tetrahydroquinazoline derivatives was proposed.
Key words: pepsin, non-natural catalytic activity, tetrahydroquinazoline, biocatalysis


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