摘要/Abstract

从疣孢菌NS0172的发酵产物中分离得到2个新的邻二烷基取代芳香酸类化合物1和2.通过一维和二维NMR数据解析及高分辨质谱分析确定了化合物1和2的化学结构.利用噻唑蓝（MTT）比色法测定了化合物1和2的细胞毒活性，结果显示化合物1对人肝癌细胞SMMC-7721具有一定的细胞毒性（IC₅₀ 7.74 μmol·L^-1）.利用滤纸片法测定了化合物1和2的抗细菌和抗真菌活性，结果显示两者在40 μg/disc条件下均无抗菌活性.这是首次报道疣孢菌代谢产生邻二烷基取代芳香酸类化合物，为后续开展相关生物合成机制研究奠定了基础.
关键词: 疣孢菌, 放线菌, 天然产物, 邻二烷基取代芳香酸
Two new ortho-dialkyl-substituted aromatic acids 1 and 2 were isolated from Verrucosispora sp. NS0172. The chemical structures of 1 and 2 were determined by spectroscopic methods including 1D- and 2D-NMR and HR-ESIMS experiments. The cytotoxicity of compounds 1 and 2 was evaluated by methyl thiazolyl tetrazolium (MTT) assay, and compound 1 showed potent antiproliferative activity against human hepatocellular carcinoma SMMC-7721 (IC₅₀ 7.74 μmol·L^-1). Compounds 1 and 2 were tested for the antimicrobial and antifungal activities by filter paper disc diffusion assay, and both of them showed no evident activities at a dose of 40 μg/disc. This study is the first report of discovering of ortho-dialkyl-substi-tuted aromatic acids from Verrucosispora, which sets the foundation for future biosynthetic study of this class of natural products in Verrucosispora.
Key words: verrucosispora, actinomycetes, natural product, ortho-dialkyl-substituted aromatic acids


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